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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (36): 170-176.doi: 10.11924/j.issn.1000-6850.casb16050169

所属专题： 园艺

• 植物保护 农药 • 上一篇    下一篇

小菜蛾高毒力Bt.DBW902粉剂开发研究

付海燕1,朱 勋2,刘春光1,侯跃莹1,杨 晶1,杨峰山1   

  1. (1黑龙江大学生命科学学院,微生物黑龙江省高校重点实验室,哈尔滨 150080；2中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京 100193）
  • 收稿日期:2016-05-30 修回日期:2016-07-19 接受日期:2016-07-25 出版日期:2016-12-26 发布日期:2016-12-26
  • 通讯作者: 杨峰山
  • 基金资助:
    哈尔滨市科技局科技创新人才专项资金优秀学科带头人项目“小菜蛾高毒力苏云金芽孢杆菌筛选鉴定与应用研究”(2014RFXXJ050)；“十二五”国家科技计划课题“果蔬重大病虫害防控技术研究与集成示范”(2012BAD19B06)。

Microbial Inoculum of Bt.DBW902 with High Toxic to Plutella xylostella

Fu Haiyan1, Zhu Xun2, Liu Chunguang1, Hou Yueying1, Yang Jing1, Yang Fengshan1   

  1. (1Key Laboratory of Microbiology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080; 2Department of Plant Protection, Institute of Vegetables and Flowers, Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables, Chinese Academy of Agricultural Sciences, Beijing 100193)
  • Received:2016-05-30 Revised:2016-07-19 Accepted:2016-07-25 Online:2016-12-26 Published:2016-12-26

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摘要： 研究旨在为微生物菌剂研发及生产提供参考。利用小菜蛾高毒力苏云金芽孢杆菌株Bt.DBW902，构建最佳发酵条件，探索Bt粉剂制备工艺。以活菌数、活芽孢数和晶体蛋白含量为指标，采用单因子试验方法，对菌株最佳发酵条件进行优化。通过添加各种不同浓度梯度矿物填料制备Bt粉剂初型，测定粉剂特性。研究结果表明：Bt.DBW902最佳发酵条件为：接种量2%，种子液菌龄10 h，摇床转速200 r/min，初始pH 7.0~7.5，温度30℃，发酵时间48 h。该条件下活菌数达到2.99×109 CFU/mL，活芽孢数达到8.88×108 CFU/mL，晶体蛋白含量可达到20.0 mg/mL。Bt粉剂初型中，人造沸石对Bt粉剂保护作用最佳，添加比例为1.0%~3.0%时，Bt.DBW902对小菜蛾毒杀活性最强。本研究优化了Bt.DBW902发酵条件并获得了高毒力Bt粉剂初型，为Bt粉剂研发及生产奠定了基础。

关键词: 水产品, 水产品, 农药, 残留, 限量

Abstract: To provide references for development and production of microbial inoculum, the Bt.DBW902 strain with high toxic to Plutella xylostella (L.) was used to establish the optimal fermentation condition and explore the preparation technics of Bt powder. Survival strains, viable spores and protein content were used as indexes, the fermentation condition was optimized by adopting a single-factor assay. The original types of Bt powder were prepared by adding different mineral with different concentrations, then the powder characteristics were detected. Results showed that the optimal fermentation condition was as follows: inoculum quantity 2%, seed age 10 h, agitation speed 200 r/min, initial pH 7.0-7.5, temperature 30℃ and culture time 48 h. Under this fermentation condition, the viable cells could reach 2.99×109 CFU/mL, viable spores could reach 8.88×108 CFU/mL and crystal proteins could reach 20.0 mg/mL. In the original types of Bt powder, the artificial zeolite could best protect the Bt powder, and the 1.0%-3.0% dose of artificial zeolite had the strongest effect on P.xylostella. This study optimized the fermentation condition of Bt.DBW902 and obtained the original types of Bt powder which laid the foundation for development and research of Bt powder.

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