BBD-响应面法优化绿色木霉产胶霉菌素培养条件

doi:10.11924/j.issn.1000-6850.casb18090008

摘要/Abstract

摘要：

旨在优化绿色木霉产胶霉菌素培养条件。实验取绿色木霉产胶霉菌素培养条件中的5个重要影响因子,设计5因素3水平的响应面分析实验,数据用Design-Expert 8.0分析得出预测模型,验证模型的可靠性后,得出绿色木霉产胶霉菌素最佳的培养条件为:培养温度29.42℃、培养基初始pH 6.35、接种量5.44%、培养时间54.5 h、菌龄44.5 h,且在最佳培养条件下胶霉菌素效价较优化前提高了15.08%。

关键词: 绿色木霉, 胶霉菌素, 响应面分析, 优化, 培养条件

Abstract:

The aim is to optimize the cultivation conditions of producing gliotoxin by Trichoderma viride. 5 important factors in the gliotoxin cultivation from Trichoderma viride were chosen, and the response surface analysis experiment (five factors three levels) was designed. A predictive model was established through the analysis of the data by Design-Expert 8.0, and the reliability of the model was verified. The optimal condition of gliotoxin production was determined as follows: the cultivation temperature was 29.42℃, the initial pH value of the medium was 6.35, the inoculation volume was 5.4%, the cultivation was for 54.5 h, the bacterium age was 44.5 h. The titer of gliotoxin was increased by 15.08% compared with that before the optimization.

Key words: Trichoderma viride, gliotoxin, response surface analysis, optimization, cultivation condition

中图分类号:

Q815

潘冬梅, 杨传伦, 张心青, 倪建龙, 王秀芝, 王春. BBD-响应面法优化绿色木霉产胶霉菌素培养条件[J]. 中国农学通报, 2020, 36(3): 111-116.

Pan Dongmei, Yang Chuanlun, Zhang Xinqing, Ni Jianlong, Wang Xiuzhi, Wang Chun. BBD-response Surface Methodology Optimizes the Cultivation Conditions of Producing Gliotoxin by Trichoderma viride[J]. Chinese Agricultural Science Bulletin, 2020, 36(3): 111-116.

图/表 6

参考文献 21

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变异	平方和	自由度	均值	F值	P值	显著性
模型	12988.98	20	649.45	14.30	<0.0001	显著
A	639.96	1	639.96	14.09	0.0009	显著
B	1956.29	1	1956.29	43.06	<0.0001	显著
C	1925.24	1	1925.24	42.38	<0.0001	显著
D	1133.67	1	1133.67	24.96	<0.0001	显著
E	688.28	1	688.28	15.15	0. 0007	显著
AB	379.86	1	379.86	8.36	0.0078	显著
AC	33.96	1	33.93	0.75	0.3957	不显著
AD	39.06	1	39.06	0.86	0.3626	不显著
AE	115.78	1	115.78	2.55	0.1230	不显著
BC	195.86	1	195.86	4.31	0.0483	显著
BD	473.28	1	473.28	10.42	0.0035	显著
BE	56.40	1	56.40	1.24	0.2758	不显著
CD	0.16	1	0.16	3.522E-003	0.9531	不显著
CE	141.02	1	141.02	3.10	0.0903	不显著
DE	386.71	1	386.71	8.51	0.0074	显著
A²	1186.22	1	1186.22	26.11	<0.0001	显著
B²	506.66	1	506.66	11.15	0.0026	显著
C²	2620.61	1	2620.61	57.69	<0.0001	显著
D²	2900.69	1	2900.69	63.85	<0.0001	显著
E²	1646.65	1	1646.65	36.25	<0.0001	显著

变异	平方和	自由度	均值	F值	P值	显著性
模型	12988.98	20	649.45	14.30	<0.0001	显著
A	639.96	1	639.96	14.09	0.0009	显著
B	1956.29	1	1956.29	43.06	<0.0001	显著
C	1925.24	1	1925.24	42.38	<0.0001	显著
D	1133.67	1	1133.67	24.96	<0.0001	显著
E	688.28	1	688.28	15.15	0. 0007	显著
AB	379.86	1	379.86	8.36	0.0078	显著
AC	33.96	1	33.93	0.75	0.3957	不显著
AD	39.06	1	39.06	0.86	0.3626	不显著
AE	115.78	1	115.78	2.55	0.1230	不显著
BC	195.86	1	195.86	4.31	0.0483	显著
BD	473.28	1	473.28	10.42	0.0035	显著
BE	56.40	1	56.40	1.24	0.2758	不显著
CD	0.16	1	0.16	3.522E-003	0.9531	不显著
CE	141.02	1	141.02	3.10	0.0903	不显著
DE	386.71	1	386.71	8.51	0.0074	显著
A²	1186.22	1	1186.22	26.11	<0.0001	显著
B²	506.66	1	506.66	11.15	0.0026	显著
C²	2620.61	1	2620.61	57.69	<0.0001	显著
D²	2900.69	1	2900.69	63.85	<0.0001	显著
E²	1646.65	1	1646.65	36.25	<0.0001	显著