长枝木霉T6微波诱变菌株筛选及其耐盐性测定

doi:10.11924/j.issn.1000-6850.casb2020-0069

中国农学通报 ›› 2020, Vol. 36 ›› Issue (33): 66-70.doi: 10.11924/j.issn.1000-6850.casb2020-0069

• 资源·环境·生态·土壤·气象 • 上一篇下一篇

长枝木霉T6微波诱变菌株筛选及其耐盐性测定

程开勇¹^,²(), 徐秉良¹^,², 吴花玉¹^,², 陈京环¹^,², 张树武¹^,²()

¹甘肃农业大学植物保护学院,兰州 730070
²甘肃省农作物病虫害生物防治工程实验室,兰州 730070

收稿日期:2020-05-07 修回日期:2020-07-07 出版日期:2020-11-25 发布日期:2020-11-18
通讯作者: 张树武
作者简介:程开勇,男,1996年出生,陕西汉中人,硕士研究生,研究方向：微生物农药开发。通信地址：730070 甘肃兰州安宁区营门村1号甘肃农业大学植物保护学院,Tel：18793123002,E-mail： 550019184@qq.com。
基金资助:
陇原青年创新创业人才项目“木霉生物菌肥对蔬菜根结线虫的防治及其机制解析”(LYRC2019-35);甘肃省杰出青年基金“基于转录组学和CRISPR/Cas9系统介导的长枝木霉T6菌株杀线基因挖掘及其功能鉴定”(18JR3RA161)

Trichoderma longibrachiatum T6: Strains Screening with Microwave Mutagenesis and Salt-tolerance Identification

Cheng Kaiyong¹^,²(), Xu Bingliang¹^,², Wu Huayu¹^,², Chen Jinghuan¹^,², Zhang Shuwu¹^,²()

¹College of Plant Protection, Gansu Agricultural University, Lanzhou 730070
²Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070

Received:2020-05-07 Revised:2020-07-07 Online:2020-11-25 Published:2020-11-18
Contact: Zhang Shuwu

摘要/Abstract

摘要：

为了获得高效耐盐木霉菌株,本试验以长枝木霉T6菌株为出发菌株,采用微波诱变处理和NaCl溶液模拟盐胁迫条件,并结合菌丝生长速率,产孢量及菌丝干重等指标筛选最佳诱变耐盐菌株。结果表明,微波诱变对长枝木霉T6菌株生长具有明显影响,并随着微波诱变处理时间的不同其影响作用显著不同,尤其当诱变时间为60 s时,其诱变菌株的菌落直径、菌丝干重和产孢量显著高于原出发菌株。微波诱变时间为60 s条件下,获得的诱变菌株生长速率显著高于其他处理,较原出发菌株表现出较强的耐盐性,培养72 h较原出发菌株生长速率平均提高32.98%。该试验利用微波诱变处理选育和获得了高产和高效的耐盐性木霉菌株,为今后多功能木霉制剂的研发应用及推广奠定理论基础。

关键词: 长枝木霉T6菌株, 微波诱变, 筛选, 耐盐性, 生长速率

Abstract:

The aim is to obtain the high-efficiency salt tolerant Trichoderma strains. The strain of Trichoderma longibrachiatum T6 was used as the original strain. Microwave mutagenesis and NaCl solution were used to simulate the salt stress condition. The colony growth rates, sporulation and mycelium dry weight were considered as indexes to screen the optimal salt tolerant strains. The results showed that the microwave mutation had a significant effect on the growth of Trichoderma longibrachiatum T6, and the effect was different with the different time of microwave mutation. The colony diameter, mycelium dry weight and sporulation of the mutant strain were significantly higher than those of the original strain at 60 s after microwave mutation. In addition, the colony growth rate of the mutant strain was significantly faster than other treatments, and showed stronger salt tolerance than the original strain. The colony growth rate was increased by 32.98% after 72 h of culture compared with the original strain. The high-yield and high-efficiency Trichoderma strains with salt tolerance were selected and obtained in this experiment, which laid a theoretical foundation for the development and application of multifunctional Trichoderma preparation in the future.

Key words: Trichoderma longibrachiatum T6, microwave mutagenesis, screen, salt tolerance, growth rates

中图分类号:

程开勇, 徐秉良, 吴花玉, 陈京环, 张树武. 长枝木霉T6微波诱变菌株筛选及其耐盐性测定[J]. 中国农学通报, 2020, 36(33): 66-70.

Cheng Kaiyong, Xu Bingliang, Wu Huayu, Chen Jinghuan, Zhang Shuwu. Trichoderma longibrachiatum T6: Strains Screening with Microwave Mutagenesis and Salt-tolerance Identification[J]. Chinese Agricultural Science Bulletin, 2020, 36(33): 66-70.

图/表 4

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培养时间/h	诱变时间
培养时间/h	0 (CK)	15 s	30 s	45 s	60 s	75 s
48	5.35±0.03a	5.28±0.01a	5.20±0.02b	5.11±0.02c	5.03±0.02d	4.96±0.03d
72	7.83±0.02d	7.95±0.03c	8.03±0.02c	8.15±0.06b	8.27±0.03a	7.93±0.02c
96	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a

培养时间/h	诱变时间
培养时间/h	0 (CK)	15 s	30 s	45 s	60 s	75 s
48	5.35±0.03a	5.28±0.01a	5.20±0.02b	5.11±0.02c	5.03±0.02d	4.96±0.03d
72	7.83±0.02d	7.95±0.03c	8.03±0.02c	8.15±0.06b	8.27±0.03a	7.93±0.02c
96	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a

培养时间/h	NaCl浓度/(mg/mL)	诱变时间
培养时间/h	NaCl浓度/(mg/mL)	0 s (CK)	15 s	30 s	45 s	60 s	75 s
72	0	7.80±0.06b	7.79±0.02b	7.82±0.01b	8.13±0.01a	8.20±0.03a	8.16±0.01a
	20	6.05±0.01b	6.05±0.03b	6.00±0.12b	5.10±0.03c	6.60±0.03a	5.10±0.04c
	30	3.83±0.02d	3.87±0.03d	3.89±0.03d	5.10±0.06c	6.40±0.09a	5.85±0.03b
	40	3.13±0.02d	3.15±0.03d	3.25±0.05d	4.13±0.02c	5.40±0.09a	4.51±0.03b
	50	2.47±0.01cd	2.45±0.03cd	2.49±0.04c	2.92±0.01b	3.10±0.06a	2.36±0.03d
	60	2.32±0.03b	2.35±0.01b	2.32±0.02b	2.04±0.03d	2.75±0.03a	2.23±0.03c
96	0	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	20	9.00±0.00a	8.13±0.02c	9.00±0.00a	8.56±0.04b	9.00±0.00a	9.00±0.00a
	30	9.00±0.00a	6.75±0.02c	9.00±0.00a	7.88±0.04b	9.00±0.00a	9.00±0.00a
	40	4.65±0.02c	4.90±0.04b	4.13±0.03e	4.50±0.02d	9.00±0.00a	4.08±0.06e
	50	2.90±0.06d	3.80±0.07a	3.15±0.03c	3.47±0.03b	3.87±0.04a	2.82±0.01d
	60	2.62±0.04c	3.08±0.03a	2.70±0.06c	2.59±0.01c	3.15±0.03a	2.93±0.07b
120	0	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	20	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	30	9.00±0.00a	7.90±0.02b	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	40	6.25±0.02b	5.90±0.06c	5.50±0.06d	4.10±0.01f	9.00±0.00a	4.92±0.03e
	50	3.30±0.06d	3.75±0.03b	3.67±0.04bc	3.62±0.04bc	4.55±0.02a	3.60±0.06c
	60	3.48±0.04c	3.70±0.05b	3.29±0.03d	3.52±0.02c	3.85±0.02a	3.31±0.05d

培养时间/h	NaCl浓度/(mg/mL)	诱变时间
培养时间/h	NaCl浓度/(mg/mL)	0 s (CK)	15 s	30 s	45 s	60 s	75 s
72	0	7.80±0.06b	7.79±0.02b	7.82±0.01b	8.13±0.01a	8.20±0.03a	8.16±0.01a
	20	6.05±0.01b	6.05±0.03b	6.00±0.12b	5.10±0.03c	6.60±0.03a	5.10±0.04c
	30	3.83±0.02d	3.87±0.03d	3.89±0.03d	5.10±0.06c	6.40±0.09a	5.85±0.03b
	40	3.13±0.02d	3.15±0.03d	3.25±0.05d	4.13±0.02c	5.40±0.09a	4.51±0.03b
	50	2.47±0.01cd	2.45±0.03cd	2.49±0.04c	2.92±0.01b	3.10±0.06a	2.36±0.03d
	60	2.32±0.03b	2.35±0.01b	2.32±0.02b	2.04±0.03d	2.75±0.03a	2.23±0.03c
96	0	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	20	9.00±0.00a	8.13±0.02c	9.00±0.00a	8.56±0.04b	9.00±0.00a	9.00±0.00a
	30	9.00±0.00a	6.75±0.02c	9.00±0.00a	7.88±0.04b	9.00±0.00a	9.00±0.00a
	40	4.65±0.02c	4.90±0.04b	4.13±0.03e	4.50±0.02d	9.00±0.00a	4.08±0.06e
	50	2.90±0.06d	3.80±0.07a	3.15±0.03c	3.47±0.03b	3.87±0.04a	2.82±0.01d
	60	2.62±0.04c	3.08±0.03a	2.70±0.06c	2.59±0.01c	3.15±0.03a	2.93±0.07b
120	0	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	20	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	30	9.00±0.00a	7.90±0.02b	9.00±0.00a	9.00±0.00a	9.00±0.00a	9.00±0.00a
	40	6.25±0.02b	5.90±0.06c	5.50±0.06d	4.10±0.01f	9.00±0.00a	4.92±0.03e
	50	3.30±0.06d	3.75±0.03b	3.67±0.04bc	3.62±0.04bc	4.55±0.02a	3.60±0.06c
	60	3.48±0.04c	3.70±0.05b	3.29±0.03d	3.52±0.02c	3.85±0.02a	3.31±0.05d

长枝木霉T6微波诱变菌株筛选及其耐盐性测定

Trichoderma longibrachiatum T6: Strains Screening with Microwave Mutagenesis and Salt-tolerance Identification

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