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中国农学通报 ›› 2021, Vol. 37 ›› Issue (18): 65-74.doi: 10.11924/j.issn.1000-6850.casb2020-0502

所属专题: 生物技术 资源与环境

• 资源·环境·生态·土壤·气象 • 上一篇    下一篇

生物质炭配施有机肥对旱地红壤酶活性及其微生物群落组成的影响

沈芳芳1,2(), 张哲1, 袁颖红1(), 周际海3   

  1. 1南昌工程学院/江西省退化生态系统修复与流域生态水文重点实验室,南昌 330099
    2江西省水土保持科学研究院江西省土壤侵蚀与防治重点实验室,南昌 330029
    3安徽师范大学/重要生物资源保护与利用研究安徽省省级重点实验室,安徽芜湖 241000
  • 收稿日期:2020-09-24 出版日期:2021-06-25 发布日期:2021-07-13
  • 通讯作者: 袁颖红
  • 作者简介:沈芳芳,女,1986年出生,江西抚州人,讲师,博士,主要从事土壤生态方面研究。通信地址:330099 江西省南昌高新区天祥大道289号,E-mail: 275774853@qq.com
  • 基金资助:
    国家自然科学基金“生物炭有机肥堆肥对旱地红壤CH4和NO2通量的影响及微生物学机制”(31760167);“改良剂对旱地红壤碳汇的影响及其微生物学机制”(41461050);“秸秆及其生物质炭还田提升旱地红壤地力的差异与机理研究”(41661065);江西省教育厅科研技术研究项目“生物炭有机肥堆肥对旱地红壤CH4排放的影响及微生物学机制”(GJJ161100);江西省水土保持科学研究院江西省土壤侵蚀与防治重点实验室2020年度开放基金项目“生物质炭-有机肥堆肥对旱地红壤CH4和N2O通量的影响研究”;江西省2018年研究生创新专项资金项目“生物炭有机肥堆肥对旱地红壤氮库以及N2O排放影响”(YC2018-S420)

Effects of Biochar and Organic Manure Combined Application on Soil Enzyme Activities and Microbial Community Component in Upland Red Soil

Shen Fangfang1,2(), Zhang Zhe1, Yuan Yinghong1(), 3   

  1. 1Key Laboratory of Degraded Ecosystem Restoration and Watershed Ecological Hydrology, Nanchang Institute of Technology, Nanchang 330099
    2Jiangxi Provincial Key Laboratory of Soil Erosion and Prevention,Jiangxi Institute of Soil and Water Conservation, Nanchang 330029
    3Anhui Provincial Key Laboratory for the Conservation and Utilization of Important Biological Resources, Anhui Normal University, Wuhu Anhui 241000
  • Received:2020-09-24 Online:2021-06-25 Published:2021-07-13
  • Contact: Yuan Yinghong

摘要:

研究生物质炭配施有机肥对旱地的土壤养分的影响,可为旱地农作物的土壤改良提供理论依据。针对中亚热带第四纪红黏土发育的旱地红壤,本研究通过室内培养试验,向土壤中施用生物质炭配施有机肥,探索土壤微生物生物量碳和氮(MBC、MBN)、酶活性(脲酶、蔗糖酶、过氧化氢酶)和微生物群落组成的变化。试验共设置5种处理,分别为对照(CK,0 g/kg)、水稻生物质炭配施有机肥(RM,50 g/kg)、玉米生物质炭配施有机肥(CM,50 g/kg)、小麦生物质炭配施有机肥(WM,50 g/kg)和单施有机肥(M,40 g/kg)。为尽量消除误差,试验数据采用归一化处理,即实测值减CK值后除以各处理所添加的C、N量。结果表明:生物质炭配施有机肥(RM、CM、WM)处理均显著降低了旱地红壤MBC、MBN含量、脲酶活性和总PLFAs量,以WM处理的降幅最大,降幅分别是单施有机肥(M处理)的33.89%、69.03%、47.62%和23.30%;RM处理显著提高了蔗糖酶和过氧化氢酶活性,升幅分别是M处理的91.49%和28.94%。相比单施有机肥,生物质炭配施有机肥降低了土壤总PLFAs含量(平均为-16.89%)、真菌PLFA (-38.17%)、土壤真菌PLFA/细菌PLFA比值(F/B)(-40.63%)和土壤革兰氏阴性菌PLFA/革兰氏阳性菌PLFA比值(G-/G+)(-4.3%),而提高了土壤细菌PLFA (+5.18%)、Shannon-Wiener多样性指数(+0.38%)和土壤细菌压力指数(BSI,+11%)。主成分分析表明RM处理与其他处理之间差异较大。综之,不同物料生物质炭配施有机肥引起土壤微生物量、酶活性和微生物群落组成的变化差异较大,其中影响最大的是水稻生物质炭配施有机肥处理,可为温室气体减排提供参考。

关键词: 生物质炭配施有机肥, 旱地红壤, 微生物量, 土壤酶活性, 微生物群落组成

Abstract:

To investigate the effects of biochar and organic manure combined application on soil nutrients in dry land can provide a theoretical foundation for soil improvement. Taking the upland red soil derived from Quaternary red clay in the mid-subtropical zone as the material, we added different biochar-organic manure compost into upland red soil during indoor culture experiment to explore the variation of soil microbial biomass carbon and nitrogen (MBC, MBN), enzyme activities (urease, sucrase and catalase) and microbial community component. Five treatments were set up, which were control (CK, 0 g/kg), rice straw biochar-organic manure compost (RM, 50 g/kg), corn straw biochar-organic manure compost (CM, 50 g/kg), wheat straw biochar-manure compost (WM, 50 g/kg) and manure compost (M, 40 g/kg). To eliminate errors as much as possible, test data were normalized, that was the measured value minus CK value then was divided by the amount of C and N added in each treatment. The results showed that RM, CM and WM treatment significantly reduced soil MBC, MBN, urease activity and total PLFAs, and the most significance was observed in WM treatment with the decrease rates of 33.89%, 69.03%, 47.62% and 23.30% of those of M treatment. RM treatment significantly increased soil sucrase and catalase activities, with the increase rates of 91.49% and 28.94% of those of M treatment. Compared with manure compost alone, biochar-organic manure compost reduced the total soil PLFAs (-16.89%), fungal abundance (-38.17%), fungi to bacteria ratio (F/B, -40.63%), gram-negative to gram-positive bacteria ratio (G-/G+, -4.3%), while increased the relative abundance of soil bacteria (+5.18%), Shannon-Wiener index (+0.38%) of soil microorganisms and soil bacterial pressure index (BSI, +11%). Principal component analysis showed that biochar-organic manure compost had a certain effect on soil microbial community component with the most obvious effect in RM treatment. In summary, the application of different materials’ biochar-organic manure compost could cause great difference in soil microbial biomass, enzyme activities and microbial community component. Our results indicate that the effect of rice straw biochar-organic manure compost treatment is the most significant, which could provide certain references for reducing greenhouse gas emission.

Key words: biochar-organic manure combined application, upland red soil, soil microbial biomass, soil enzyme activities, microbial community component

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