接种内生菌对干旱胁迫下甘蔗的生理影响

doi:10.11924/j.issn.1000-6850.casb2021-0416

中国农学通报 ›› 2022, Vol. 38 ›› Issue (6): 42-47.doi: 10.11924/j.issn.1000-6850.casb2021-0416

所属专题：生物技术；植物保护；园艺；农业气象

接种内生菌对干旱胁迫下甘蔗的生理影响

谷书杰¹(), 钱禛锋¹, 娄永明¹^,², 沈庆庆¹, 普凤雅¹, 曾丹¹, 马豪¹, 何丽莲¹(), 李富生¹^,³()

¹云南农业大学农学与生物技术学院,昆明 650201
²耿马县地方产业发展服务中心,云南耿马 677500
³云南省作物生产与智慧农业重点实验室,昆明 650201

收稿日期:2021-04-19 修回日期:2021-08-03 出版日期:2022-02-25 发布日期:2022-03-16
通讯作者: 何丽莲,李富生
作者简介:谷书杰,男,1996年出生,河南信阳人,在读硕士研究生,研究方向:作物种质资源评价与利用。通信地址:650201 云南省昆明市盘龙区云南农业大学学生宿舍20栋,E-mail: 1316648272@qq.com。
基金资助:
云南省现代农业甘蔗产业技术体系建设专项(2020-2021)

Physiological Effects of Inoculated Endophytes on Sugarcane Under Drought Stress

GU Shujie¹(), QIAN Zhenfeng¹, LOU Yongming¹^,², SHEN Qingqing¹, PU Fengya¹, ZENG Dan¹, MA Hao¹, HE Lilian¹(), LI Fusheng¹^,³()

¹College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201
²Gengma County Local Industry Development Service Center, Gengma, Yunnan 677500
³The Key Laboratory for Crop Production and Smart Agriculture of Yunnan Province, Kunming 650201

Received:2021-04-19 Revised:2021-08-03 Online:2022-02-25 Published:2022-03-16
Contact: HE Lilian,LI Fusheng

摘要/Abstract

摘要：

探索内生菌对甘蔗抗旱能力的影响,以期为开发利用甘蔗内生菌抗旱性功能菌株提供理论依据和技术支撑。以课题组前期分离鉴定的6株甘蔗内生菌为供试菌株,甘蔗品种‘ROC22’幼苗接种7天后进行干旱胁迫,然后取完全展开的第一叶叶片测定相关生理指标。结果表明,E3、O9和YC89均有较高的ACC脱氨酶活性,不同甘蔗内生菌菌株对甘蔗抗旱性的影响有明显差异。E3、O9和YC89在提高甘蔗耐旱能力方面效果显著,主要表现在降低细胞膜损伤、增加渗透调节物质的含量、降低膜脂过氧化程度和增加SOD、POD活性等方面。试验发现,产ACC脱氨酶的甘蔗内生菌能更有效提高甘蔗抗旱能力。

关键词: 甘蔗, 甘蔗内生菌, 干旱胁迫, 生理指标, ACC脱氨酶

Abstract:

The purpose of this study is to explore the influence of sugarcane endophytes on its drought resistance, and to provide a theoretical basis and technical support for the development and utilization of sugarcane endophytes with drought-resistant functional strains. Six sugarcane endophytes isolated and identified by the research group were used as test strains. Drought stress was simulated for seedlings of sugarcane variety ‘ROC22’ 7 days after the inoculation, and then fully expanded first leaf was taken to determine the relevant physiological indicators. The results show that E3, O9 and YC89 all have high ACC deaminase activity, and the effects of different sugarcane endophytic strains on sugarcane drought resistance are significantly different. E3, O9 and YC89 have significant effects on improving the drought tolerance of sugarcane, mainly in terms of reducing cell membrane damage, increasing the content of osmotic adjustment substances, reducing the degree of membrane peroxidation, and increasing the activity of SOD and POD. This experiment found that the sugarcane endophytes producing ACC deaminase can effectively improve the drought resistance of sugarcane.

Key words: sugarcane, sugarcane endophytes, drought stress, physiological index, ACC deaminase

中图分类号:

S566.1

谷书杰, 钱禛锋, 娄永明, 沈庆庆, 普凤雅, 曾丹, 马豪, 何丽莲, 李富生. 接种内生菌对干旱胁迫下甘蔗的生理影响[J]. 中国农学通报, 2022, 38(6): 42-47.

GU Shujie, QIAN Zhenfeng, LOU Yongming, SHEN Qingqing, PU Fengya, ZENG Dan, MA Hao, HE Lilian, LI Fusheng. Physiological Effects of Inoculated Endophytes on Sugarcane Under Drought Stress[J]. Chinese Agricultural Science Bulletin, 2022, 38(6): 42-47.

图/表 6

参考文献 46

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菌株	ACC脱氨酶活性/(U/mg)
E3	0.0490±0.0044cC
O9	0.0453±0.0025cBC
C12	0.0037±0.0006aA
C9	0.0017±0.0006aA
C6	0.0010±0aA
YC89	0.0390±0.007bB
F值	131.674^**
P值	<0.01

菌株	ACC脱氨酶活性/(U/mg)
E3	0.0490±0.0044cC
O9	0.0453±0.0025cBC
C12	0.0037±0.0006aA
C9	0.0017±0.0006aA
C6	0.0010±0aA
YC89	0.0390±0.007bB
F值	131.674^**
P值	<0.01

菌株	土壤含水量/%	菌株	土壤含水量/%
E3	20.15±0.09a	YC89	20.68±0.12a
O9	20.23±0.05a	CK	20.74±0.68a
C12	20.46±0.15a	F值	1.196
C9	20.51±0.47a	P值	>0.05
C6	20.57±0.32a

菌株	土壤含水量/%	菌株	土壤含水量/%
E3	20.15±0.09a	YC89	20.68±0.12a
O9	20.23±0.05a	CK	20.74±0.68a
C12	20.46±0.15a	F值	1.196
C9	20.51±0.47a	P值	>0.05
C6	20.57±0.32a

菌株	叶片含水量/%	相对电导率/%	叶绿素含量/(mg/g)
E3	70.45±0.49aA	4.14±0.09aA	7.05±0.01b
O9	70.05±0.84aA	4.26±0.03aA	7.02±0.05ab
C12	70.37±0.59aA	6.31±0.12cC	6.94±0.05a
C9	72.88±0.2bB	7.21±0.1dD	6.98±0.06ab
C6	72.28±1.02bB	6.26±0.04cC	6.97±0.11ab
YC89	77.04±0.4cC	4.69±0.07bB	6.93±0.03a
CK	70.16±0.22aA	7.25±0.03dD	6.92±0.03a
F值	51.958^**	963.319^**	2.271
P值	<0.01	<0.01	>0.05

接种内生菌对干旱胁迫下甘蔗的生理影响

Physiological Effects of Inoculated Endophytes on Sugarcane Under Drought Stress

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菌株	可溶性糖含量/(mg/g)	可溶性蛋白含量/(mg/g)	Pro含量/(µg/g)
E3	24.27±0.04eD	60.09±0.23dD	100.00±0.95dD
O9	22.26±0.08cC	55.65±0.62bcBC	97.64±0.72dD
C12	22.48±0.14dC	56.43±0.59cC	68.09±1.79aA
C9	21.79±0.14bB	54.86±0.08abAB	76.11±2.12cC
C6	22.49±0.05dC	55.02±0.28abAB	69.82±1.18abAB
YC89	27.43±0.16fE	56.30±0.30cC	110.21±0.72eE
CK	21.02±0.11aA	54.36±0.72aA	72.18±2.6bB
F值	1059.152^**	52.029^**	350.34^**
P值	<0.01	<0.01	<0.01

菌株	MDA含量/(nmol/g)	菌株	MDA含量/(nmol/g)
E3	14.38±0.22aA	YC89	14.73±0.71aAB
O9	13.57±0.27aA	CK	19.82±0.43dD
C12	18.00±0.4cCD	F值	27.852^**
C9	16.40±0.27bBC	P值	<0.01
C6	18.04±1.72cCD

菌株	POD活性/(U/g)	SOD活性/(U/g)
E3	3090.97±41.18cC	365.57±2.48c
O9	2686.76±41.18bB	350.58±16.16bc
C12	2758.09±41.18bB	334.58±9.64ab
C9	2472.77±41.18aA	345.01±9.3abc
C6	2710.54±71.33bB	347.60±4.03abc
YC89	3828.04±41.18dD	352.22±22.53bc
CK	2781.87±71.33bB	325.65±2.16a
F值	224.303^**	18.667^*
P值	<0.05	<0.05

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