环境因子及内源物质对果树中花青素调控的研究进展

doi:10.11924/j.issn.1000-6850.casb2022-0540

中国农学通报 ›› 2023, Vol. 39 ›› Issue (19): 51-57.doi: 10.11924/j.issn.1000-6850.casb2022-0540

环境因子及内源物质对果树中花青素调控的研究进展

王硕¹(), 郑秀文¹, 刘冠¹(), 张萌萌²^,³()

¹ 黑龙江大学现代农业与生态环境学院，哈尔滨 150080
² 黑龙江大学/农业微生物技术教育部工程研究中心，哈尔滨 150500
³ 黑龙江大学生命科学学院/黑龙江省寒地生态修复与资源利用重点实验室，哈尔滨 150080

收稿日期:2022-07-06 修回日期:2022-12-27 出版日期:2023-07-05 发布日期:2023-07-03
通讯作者: 刘冠，女，1989年出生，黑龙江双鸭山人，讲师，博士，研究方向：植物分子育种。通信地址：150080 哈尔滨市南岗区学府路74号黑龙江大学现代农业与生态环境学院，E-mail：2019057@hlju.edu.cn。张萌萌，1989年出生，黑龙江哈尔滨人，副教授，博士，研究方向：植物生理及微生物生态。通信地址：150080 哈尔滨市南岗区学府路74号黑龙江大学生命科学学院，E-mail：2019047@hlju.edu.cn。
作者简介:
王硕，男，2001年出生，河南开封人，本科，研究方向：种子科学与工程。通信地址：150080 哈尔滨市南岗区学府路74号黑龙江大学现代农业与生态环境学院，E-mail：w20206856@163.com。
基金资助:
黑龙江省自然科学基金优秀青年项目“番茄抗叶霉病基因cf-10的精细定位与应答机制分析”(YQ2020C038); 国家自然科学基金青年科学基金项目“模拟氮沉降下阔叶红松林凋落物层氮素转化与相关功能微生物特性研究”(32001126); 黑龙江省省属高校基本科研业务费科研项目“WRKY转录因子家族在番茄与叶霉菌互作中的功能分析”(2020-KYYWF-1048); 黑龙江省大学生创新创业训练计划项目“超声波-微波辅助提取蓝靛果花青素工艺优化及微胶囊化研究”(2022069); 新农科背景下可持续发展理念融入创新创业教育的研究与实践(SJGY20210719); 黑龙江大学第三批课程思政建设项目植物生理学B。

Research Progress on Regulation of Anthocyanins in Fruit Trees by Environmental Factors and Endogenous Substances

WANG Shuo¹(), ZHENG Xiuwen¹, LIU Guan¹(), ZHANG Mengmeng²^,³()

¹ College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
² Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education/ Heilongjiang University,Harbin 150500
³ Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region/ School of Life Sciences, Heilongjiang University, Harbin 150080

Received:2022-07-06 Revised:2022-12-27 Online:2023-07-05 Published:2023-07-03

摘要/Abstract

摘要：

花青素的合成途径基本被确定，但是关于环境因子及内源物质对花青素积累的影响仍旧缺乏全面系统的阐述。本研究根据国内外的研究进展，总结光照、温度、矿质元素等环境因素以及内源激素等物质对果树中花青素积累的影响，发现较强光照、低温、部分矿质元素、水分胁迫、酸性环境、糖类以及乙烯等的内源激素对花青素的稳定性具有保护作用，并能促进花青素的合成。此类研究可为果树果实色泽、营养品质的进一步改良提供理论基础，为果树品种的选育与研发提供参考方向，同时也为果树中花青素的提取、加工、运输和贮藏过程提供理论指导，促进优良、天然的植物化学物质在果树中的充分积累及综合利用。而对于环境因子和内源激素在花青素调控网络中的具体作用机制，在未来仍需要借助分子生物学、表观遗传学等生物技术进行深入研究。

关键词: 花青素, 环境因子, 内源激素, 果树, 光照, 生长素

Abstract:

The synthetic pathway of anthocyanins has been roughly determined, but there is still a lack of comprehensive and systematic elaboration of the effects of environmental factors and endogenous substances on the accumulation of anthocyanins. According to the domestic and foreign research progress, by summarizing the effects of light, temperature, mineral elements and other environmental factors, as well as endogenous hormones and other substances on the accumulation of anthocyanins in fruit trees, it was found that strong light, low temperature, some mineral elements, moderate drought, acidic environment, sugars and ethylene had a protective effect on the stability of anthocyanins, and could promote the synthesis of anthocyanins. Such research can provide a theoretical basis for the further improvement of fruit color and nutritional quality of fruit trees, provide a reference direction for the breeding, research and development of fruit varieties, and also provide theoretical guidance for the extraction, processing, transportation and storage of anthocyanins in fruit trees, so as to promote the full accumulation and comprehensive utilization of fine and natural phytochemicals in fruit trees. However, the specific mechanisms of environmental factors and endogenous hormones in the anthocyanin regulatory network still need to be further studied with the help of molecular biology, epigenetics and other biotechnology in the future.

Key words: anthocyanin, environmental factors, endogenous hormones, fruit tree, illumination, auxin

王硕, 郑秀文, 刘冠, 张萌萌. 环境因子及内源物质对果树中花青素调控的研究进展[J]. 中国农学通报, 2023, 39(19): 51-57.

WANG Shuo, ZHENG Xiuwen, LIU Guan, ZHANG Mengmeng. Research Progress on Regulation of Anthocyanins in Fruit Trees by Environmental Factors and Endogenous Substances[J]. Chinese Agricultural Science Bulletin, 2023, 39(19): 51-57.

图/表 2

参考文献 69

[1]	SMERIGLIO A, BARRECA D, BELLOCCO E, et al. Chemistry, pharmacology and health benefits of anthocyanins[J]. Phytotherapy research, 2016, 30(8):1265-1286. doi: 10.1002/ptr.5642 pmid: 27221033
[2]	郝俊光, 梁振荣, 银书, 等. 高效液相色谱法测定罗城‘桂葡1号'毛葡萄酿酒过程中9种酚类物质的变化[J]. 食品工业科技, 2021, 42(21):8.
[3]	王欣, 张天柱. 园艺作物花青素合成调控研究进展[J]. 生物技术进展, 2022, 12(1):10-16. doi: 10.19586/j.2095-2341.2021.0125
[4]	DAVIES K M, ALBERT N W, SCHWINN K E. From landing lights to mimicry: the molecular regulation of flower colouration and mechanisms for pigmentation patterning[J]. Functional plant biology, 2012, 39(8):619-638. doi: 10.1071/FP12195 pmid: 32480814
[5]	FABIOLA, INIGUEZ-FRANCO, HERLINDA, et al. Antioxidant activity and diffusion of catechin and epicatechin from antioxidant active films made of poIy(L-lactic acid)[J]. Journal of agricultural and food chemistry, 2012, 60(26):6515-6523. doi: 10.1021/jf300668u URL
[6]	NIU S S, XU C J, ZHANG W S, et al. Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3-MYB transcription factor[J]. Planta, 2010, 231(4):887-899. doi: 10.1007/s00425-009-1095-z URL
[7]	ZHANG Y, XU S, CHENG Y, et al. Transcriptome profiling of anthocyanin-related genes reveals effects of light intensity on anthocyanin biosynthesis in red leaf lettuce[J]. Peerj, 2018, 6(4):e4607. doi: 10.7717/peerj.4607 URL
[8]	FENG F, LI M, MA F, et al. Phenylpropanoid metabolites and expression of key genes involved inanthocyanin biosynthesis in the shaded peel of apple fruit in response to sun exposure[J]. Plant physiology and biochemistry, 2013, 69:54-61. doi: 10.1016/j.plaphy.2013.04.020 URL
[9]	FENG S, WANG Y, YANG S, et al. Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10[J]. Planta, 2010, 232(1):245-255. doi: 10.1007/s00425-010-1170-5 URL
[10]	JU Z, YUAN Y, LIU C, et al. Dihydroflavonol reductase activity and anthocyanin accumulation in ‘Delicious', ‘Golden Delicious' and ‘Indo' apples[J]. Scientia horticulturae, 1997, 70(1):31-43. doi: 10.1016/S0304-4238(97)00040-X URL
[11]	柳蕴芬, 刘莉, 段艳欣, 等. 光对红肉桃果肉红色形成的影响[J]. 中国农学通报, 2010(13):4.
[12]	俞明亮. 苹果花青苷色素的形成[J]. 北方果树, 1992(4):3.
[13]	YAMAGISHI M. A novel R2R3-MYB transcription factor regulates light-mediated floral and vegetative anthocyanin pigmentation patterns in Lilium regale[J]. Molecular breeding, 2016, 36(1):3. doi: 10.1007/s11032-015-0426-y URL
[14]	MAIER A, HOECKER U. COP1/SPA ubiquitin ligase complexes repress anthocyanin accumulation under low light and high light conditions[J]. Plant signaling & behavior, 2015, 10(1).
[15]	ZHENG X T, CHEN Y L, ZHANG X H, et al. ANS-deficient Arabidopsis is sensitive to high light due to impaired anthocyanin photoprotection[J]. Functional plant biology, 2019, 46:756-765. doi: 10.1071/FP19042 URL
[16]	ÇIĞDEM U P, TOKLUCU A K. Effect of UV-C light on anthocyanin content and other quality parameters of pomegranate juice[J]. Journal of food composition & analysis, 2011, 24(6):790-795.
[17]	KIM M J, KIM P, CHEN Y, et al. Blue and UV-B light synergistically induce anthocyanin accumulation by co-activating nitrate reductase gene expression in anthocyanin fruit (Aft) tomato[J]. Plant biology, 2021, 23(S1):210-220. doi: 10.1111/plb.v23.s1 URL
[18]	WANG L, LUO Z, YANG M, et al. The action of RED light: Specific elevation of pelargonidin-based anthocyanin through ABA-related pathway in strawberry[J]. Postharvest biology and technology, 2022, 186:111835. doi: 10.1016/j.postharvbio.2022.111835 URL
[19]	解潇冬, 刘晓莹, 汪文杰, 等. 光质对红阳猕猴桃愈伤组织生长速度和花青素合成的影响[J]. 山西农业科学, 2021, 49(10):7.
[20]	SHAKED-SACHRAY L, WEISS D, REUVENI M, et al. Increased anthocyanin accumulation in aster flowers at elevated temperatures due to magnesium treatment[J]. Physiologia plantarum, 2002, 114(4):559-565. doi: 10.1034/j.1399-3054.2002.1140408.x URL
[21]	金周雨, 孟展冰, 郭凌霄, 等. 蓝靛果花青素稳定性研究[J]. 北方农业学报, 2019, 47(6):5.
[22]	FANG Z Z, WANG L K, JIANG C C, et al. Postharvest temperature and light treatments induce anthocyanin accumulation in peel of 'Akihime' plum (Prunus salicina Lindl.) via transcription factor PsMYB10.1[J]. Postharvest biology and technology, 2021,179.
[23]	MORI K, GOTO-YAMAMOTO N, KITAYAMA M, et al. Loss of anthocyanins in red-wine grape under high temperature[J]. Journal of experimental botany, 2007, 58(8):1935. doi: 10.1093/jxb/erm055 pmid: 17452755
[24]	BU Y F, WANG S, LI C Z, et al. Transcriptome analysis of apples in high-temperature treatments reveals a role of MdLBD37 in the inhibition of anthocyanin accumulation[J]. International journal of molecular sciences, 2022, 23(7):3766. doi: 10.3390/ijms23073766 URL
[25]	YU L, SUN Y, ZHANG X, et al. ROS1 promotes low temperature-induced anthocyanin accumulation in apple by demethylating the promoter of anthocyanin-associated genes[J]. Horticulture research, 2022,9.
[26]	李炎林, 朱杰辉, 唐前瑞, 等. Al³⁺对红檵木叶色生理变化的影响[J]. 湖南林业科技, 2008, 35(2):8-11.
[27]	潘芝梅, 徐金刚, 卢刚, 等. 李果实发育期矿质元素和果皮花青素含量的变化规律及相关性分析[J]. 浙江林业科技, 2009, 29(4):21-24.
[28]	周开兵, 梁柱, 黄海林. 叶面喷施磷、钾、钙对三月红荔枝果实品质和着色的影响[J]. 亚热带植物科学, 2007, 36(1):27-30.
[29]	高宇, 刘怡菲, 苏宏伟, 等. 软枣猕猴桃花青素加工稳定性研究[J]. 辽宁林业科技, 2019(3):17-19.
[30]	孟庆昕, 高逸雯, 姚思敏蔷, 等. 金属离子对黑果枸杞中花青素颜色稳定性的影响[J]. 湖北农业科学, 2018, 57(22):113-117.
[31]	赵立仪, 李路宁, 申芮萌, 等. 酰基化蓝莓花青素的稳定性研究[J]. 食品工业科技, 2014, 35(22):299-304.
[32]	OLIVIER D, JULIE-ANNE F. The chemical reactivity of anthocyanins and its consequences in food science and nutrition[J]. Molecules, 2018, 23(8):1970. doi: 10.3390/molecules23081970 URL
[33]	王虹. 夏秋季节干旱胁迫对红叶桃光合特性及相关生理指标的影响[D]. 南京: 南京农业大学, 2008.
[34]	孙建霞, 张燕, 胡小松, 等. 花色苷的结构稳定性与降解机制研究进展[J]. 中国农业科学, 2009, 42(3):996-1008.
[35]	唐前瑞, 陈德富, 陈友云, 等. 红檵木叶色变化的生理生化研究[J]. 林业科学, 2006, 42(2):111-115.
[36]	刘恺媛, 王茂良, 辛海波, 等. 植物花青素合成与调控研究进展[J]. 中国农学通报, 2021, 37(14):41-51. doi: 10.11924/j.issn.1000-6850.casb2020-0390
[37]	王秋霜, 李思颖, 李家贤, 等. 红紫芽茶叶花青素提取分离及pH稳定性初探[J]. 中国农学通报, 2014, 30(9):291-296.
[38]	汤晶, 温宁红, 倪刚, 等. 探究黑枸杞中花青素稳定性的生活化实验设计[J]. 化学教育(中英文), 2018, 39(13):54-58.
[39]	李媛, 孙阳昭, 陈芳, 等. 红莓花青素稳定性和色差变化的研究[J]. 食品与发酵工业, 2008, 34(12):35.
[40]	庞学群, 张昭其, 段学武, 等. 氧化还原物质对荔枝果皮花色素苷稳定性的影响[J]. 华南农业大学学报, 2001, 22(2):15-17.
[41]	SAURE Max C. External control of anthocyanin formation in apple[J]. Scientia horticulturae, 1990, 42(3):181-218. doi: 10.1016/0304-4238(90)90082-P URL
[42]	包刘媛, 李香勇, 李洪, 等. 环境条件对花青素苷稳定性的影响[A].//国际茶花协会.大理国际茶花大会论文集[C]. 2016:346-355.
[43]	MEGANINGTYAS W, ALAUHDIN M. Extraction of anthocyanin from the dragon fruit (Hylocereus costaricensis) peel and its application as a natural indicator of acid-base titration[J]. Agritech, 2021, 41(3):278-284. doi: 10.22146/agritech.52197 URL
[44]	肖婧. 探究性实验设计:紫甘蓝花青素的提取及其酸碱指示剂应用[J]. 广东化工, 2019(6):104-105.
[45]	吴晓红, 马英, 康志宁, 等. 利用黑枸杞自制酸碱指示剂的趣味化实验设计[J]. 教育与装备研究, 2018, 34(6):82-83.
[46]	庄维兵, 刘天宇, 束晓春, 等. 植物体内花青素苷生物合成及呈色的分子调控机制[J]. 植物生理学报, 2018, 54(11):15.
[47]	隋秀奇, 姜志红. 制约苹果红色形成的要素[J]. 落叶果树, 1995, 3:36-36.
[48]	张学英, 张上隆, 骆军, 等. 果实花色素苷合成研究进展[J]. 果树学报, 2004(5):456-460.
[49]	HUANG Z, WANG Q, XIA L, et al. Preliminarily exploring of the association between sugars and anthocyanin accumulation in apricot fruit during ripening[J]. Scientia horticulturae, 2019, 248:112-117. doi: 10.1016/j.scienta.2019.01.012 URL
[50]	孙倩怡, 任珅, 鲁宝君, 等. 蓝莓花青素的稳定性研究[J]. 营养学报, 2017, 39(4).
[51]	ZHANG Q, YANG J, WANG L, et al. Correlation between soluble sugar and anthocyanin and effect of exogenous sugar on coloring of ‘Hongtaiyang' pear[J]. Journal of fruit science, 2013, 30(2):248-253.
[52]	孙倩怡. 蓝莓中花青素的提取和稳定性的研究及其脂质体的制备[D]. 长春: 吉林农业大学, 2018.
[53]	XIONG Y. Relationship between sucrose metabolism and anthocyanin biosynthesis during ripening in Chinese bayberry fruit[J]. Journal of agricultural & food chemistry, 2014, 62:10522-10528.
[54]	赵杰堂. 激素调控植物花青素合成分子机制的研究进展[J]. 分子植物育种, 2016, 14(7).
[55]	MEYER H J, STADEN J. The in vitro production of an anthocyanin from callus cultures of Oxalis linearis[J]. Plant cell tissue & organ culture, 1995, 40(1):55-58.
[56]	ZHOU L L, ZENG H N, SHI M Z, et al. Development of tobacco callus cultures over expressing Arabidopsis PAP1/MYB75 transcription factor and characterization of anthocyanin biosynthesis[J]. Planta, 2008, 229(1):37-51. doi: 10.1007/s00425-008-0809-y URL
[57]	XIAO H, JI Y T, WANG R, et al. Effect of auxin, cytokinin and nitrogen on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f. niedzwetzkyana)[J]. Plant cell, tissue and organ culture, 2015, 120(1):325-337. doi: 10.1007/s11240-014-0609-y URL
[58]	MORO, LAÍS, HASSIMOTTO N M A, et al. Postharvest auxin and methyl jasmonate effect on anthocyanin biosynthesis in red raspberry (Rubus idaeus L.)[J]. Journal of plant growth regulation, 2017, 36(3):773-782. doi: 10.1007/s00344-017-9682-x URL
[59]	CHENG Y, WANG N, FENG H, et al. Auxin regulates anthocyanin biosynthesis through the Aux/IAA-ARF signaling pathway in apple[J]. Horticulture research, 2018,5.
[60]	QU S S, LI M M, WANG G, et al. Application of ABA and GA₃ alleviated browning of litchi (Litchi chinensis Sonn.) via different strategies[J]. Postharvest biology and technology, 2021, 181:111672. doi: 10.1016/j.postharvbio.2021.111672 URL
[61]	LI W F, MAO J, YANG S J, et al. Anthocyanin accumulation correlates with hormones in the fruit skin of ‘Red Delicious' and its four generation bud sport mutants[J]. BMC plant biology, 2018, 18(1).
[62]	OH H D, YU D J, SUN W C, et al. Abscisic acid stimulates anthocyanin accumulation in ‘Jersey' highbush blueberry fruits during ripening[J]. Food chemistry, 2018, 244:403-407. doi: 10.1016/j.foodchem.2017.10.051 URL
[63]	LI G, ZHAO J, QIN B, et al. ABA mediates development-dependent anthocyanin biosynthesis and fruit coloration in Lycium plants[J]. BMC plant biology, 2019, 19(1).
[64]	尹金华, 高飞飞, 胡桂兵, 等. ABA和乙烯对荔枝果实成熟和着色的调控[J]. 园艺学报, 2001, 28(1):65-67.
[65]	NI J B, ZHAO Y, TAO R Y, et al. Ethylene mediates the branching of the jasmonate-induced flavonoid biosynthesis pathway by suppressing anthocyanin biosynthesis in red Chinese pear fruits[J]. Plant biotechnology journal, 2020, 18(5):1223-1240. doi: 10.1111/pbi.13287 pmid: 31675761
[66]	李艳芳, 聂佩显, 张鹤华, 等. 蓝莓果实花青苷积累与内源激素含量动态变化[J]. 北京林业大学学报, 2017, 39(2):64-71.
[67]	YU J, QIU K, SUN W, et al. A long noncoding RNA functions in high-light-induced anthocyanin accumulation in apple by activating ethylene synthesis[J]. Plant physiology, 2022(1):1.
[68]	杨琳, 王宇, 杨剑飞, 等. 花青素积累相关负调控因子的研究进展[J]. 园艺学报, 2014(9):1873-1884.
[69]	DAS P K, SHIN D H, CHOI S B, et al. Cytokinins enhance sugar-induced anthocyanin biosynthesis in Arabidopsis[J]. Molecules and cells, 2012, 34(1):93-101. doi: 10.1007/s10059-012-0114-2 URL

环境因子及内源物质对果树中花青素调控的研究进展

Research Progress on Regulation of Anthocyanins in Fruit Trees by Environmental Factors and Endogenous Substances

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