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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (24): 116-121.doi: 10.11924/j.issn.1000-6850.casb2023-0749

• 生物科学 • 上一篇    下一篇

菊芋HtMYB2基因VIGS体系构建与功能验证

王莹1,2(), 李娟1,2, 孙雪梅1,2()   

  1. 1 青海大学农林科学院，西宁 81000
    2 青藏高原种质资源研究与利用实验室，西宁 81000
  • 收稿日期:2023-10-04 修回日期:2024-01-15 出版日期:2024-08-20 发布日期:2024-08-20
  • 通讯作者:
    孙雪梅，女，1980年出生，青海西宁人，副研究员，博士，研究方向：园艺作物遗传育种研究。通信地址：81000 青海省西宁市城北区宁大路251号 青海大学农林科学院，Tel：13997091543，E-mail：。
  • 作者简介:

    王莹，女，1994年出生，辽宁朝阳人，博士研究生，研究方向：分子植物育种。通信地址：81000 青海省西宁市城北区宁大路251号 青海大学农林科学院，Tel：18597128601，E-mail：。

  • 基金资助:
    青海省科技计划项目“菊芋花青素代谢关键基因鉴定及其分子调控机制”(2021-ZJ-928)

HtMYB2 Gene of Helianthus tuberosus L.: VIGS System Construction and Functional Validation

WANG Ying1,2(), LI Juan1,2, SUN Xuemei1,2()   

  1. 1 Academy of Agriculture & Forestry, Qinghai University, Xining 81000
    2 Laboratory for Research and Utilization of Germplasm Resources in Qinghai-Tibet Plateau, Xining 81000
  • Received:2023-10-04 Revised:2024-01-15 Published:2024-08-20 Online:2024-08-20

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摘要：

颜色作为一种重要的农艺性状，影响作物的价值。紫皮菊芋与白皮菊芋相比，具有较好的外观颜色、营养价值和商品价值。本研究旨在探究菊芋块茎表皮颜色形成的潜在机制。通过前期转录组测序技术对紫皮和白皮菊芋块茎表皮分析，筛选得到与花青素合成代谢相关转录因子HtMYB2。本研究以紫皮菊芋品种为材料，利用VIGS技术将HtMYB2进行基因沉默，对其功能进行验证。结果表明侵染后的菊芋块茎表皮颜色变浅，花青素含量显著下降为15.34 mg/g；同时RT-PCR结果显示HtMYB2基因表达量为2.06，与对照组相比显著降低，沉默效率为73.96 %，推测HtMYB2基因的沉默使菊芋块茎中花青素基因的表达受阻，花青素合成功能减弱，导致花青素含量降低，表明HtMYB2基因在花青素合成途径中起正调控作用。本研究为菊芋块茎表皮花青素的生物合成机理提供理论依据，并进一步揭示了该基因在调控块茎颜色和花青素合成途径中的重要性。

关键词: 菊芋, HtMYB2, 花青素, 病毒介导的基因沉默(VIGS), 块茎表皮颜色, 逆转录聚合酶链反应, 基因表达, 生物合成途径

Abstract:

Color is an important agronomic trait that affects the value of crops. The purple-skinned Jerusalem artichoke has superior appearance, nutritional value, and commercial value compared to the white-skinned variety. To explore the mechanism of color formation in Jerusalem artichoke tubers, we utilized transcriptome sequencing technology to study both purple and white varieties. We identified the transcription factor HtMYB2, which is associated with anthocyanin synthesis and metabolism. In this study, the HtMYB2 gene in a purple Jerusalem artichoke variety was silenced using virus-induced gene silencing (VIGS) technology to verify its function. Results showed that the tuber epidermis color lightened after infection, anthocyanin content decreased to 15.34 mg/g, HtMYB2 gene expression was 2.06, and silencing efficiency reached 73.96% compared to the control group. It was speculated that silencing the HtMYB2 gene blocked the expression of anthocyanin-related genes in Jerusalem artichoke tubers, leading to reduced anthocyanin synthesis. The HtMYB2 gene positively regulated the anthocyanin synthesis pathway. This study provides a theoretical basis for understanding anthocyanin biosynthesis in the epidermis of Jerusalem artichoke tubers and highlights the importance of HtMYB2 in regulating tuber color and the anthocyanin biosynthesis pathway.

Key words: Helianthus tuberosus L., HtMYB2, anthocyanin, virus-induced gene silencing (VIGS), tuber epidermis color, reverse transcription polymerase chain reaction (rt-pcr), gene expression, biosynthetic pathway