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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (24): 21-27.doi: 10.11924/j.issn.1000-6850.casb2023-0344

• 农学·农业基础科学 • 上一篇    下一篇

CaCl2引发对菜豆幼苗生长、光合参数及生理生化的影响

沈祺(), 冯国军, 刘大军, 卫梦丹, 刘畅, 杨晓旭()   

  1. 黑龙江大学现代农业与生态环境学院，哈尔滨 150080
  • 收稿日期:2023-05-05 修回日期:2023-07-15 出版日期:2024-08-20 发布日期:2024-08-20
  • 通讯作者:
    杨晓旭，男，1988年出生，吉林松原人，副研究员，博士，研究方向；蔬菜遗传育种与生物技术。通信地址；150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学现代农业与生态环境学院，Tel：15546226629，E-mail：。
  • 作者简介:

    沈祺，男，1996年出生，江苏南通人，硕士在读，研究方向：蔬菜遗传育种与生物技术。通信地址：150080 黑龙江省哈尔滨市学府路74号 黑龙江大学，Tel：0451-86609482，E-mail：。

  • 基金资助:
    黑龙江省重点研发计划项目专项课题计划“主要蔬菜作物分子辅助育种决策与新品系培育研究”(SC2022ZX02C0202); 农业农村部政府购买合同“普通菜豆种质资源品质性状鉴定”(19240656); 2022年度省属高校基本科研业务费项目(2022-KYYWF-1071)

Effects on Growth, Photosynthetic Parameters, Physiology and Biochemistry of Bean Seedlings: CaCl2 Initiation

SHEN Qi(), FENG Guojun, LIU Dajun, WEI Mengdan, LIU Chang, YANG Xiaoxu()   

  1. College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
  • Received:2023-05-05 Revised:2023-07-15 Published:2024-08-20 Online:2024-08-20

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摘要：

本研究旨在探讨氯化钙（CaCl2）引发对菜豆幼苗生长的影响，并评估其在生理生化层面上的作用。实验以菜豆‘将军’为材料，采用40 mmol/L的CaCl2溶液对菜豆种子进行12 h引发处理。结果显示，相较于未引发处理的干种子对照组（CK1）和蒸馏水处理对照组（CK2），经CaCl2引发12 h后的菜豆，其幼苗干重分别显著增加了38.8%和19.2%。净光合速率较2对照组分别提高了25.8%和16.4%。淀粉酶(α+β)总活力亦显著增加，相比2对照组分别提高了79.9%和15.3%。抗氧化酶如超氧化物歧化酶（SOD）、过氧化物酶（POD）以及过氧化氢酶（CAT）活性均呈上升趋势，尤其是CAT活性的提升最为显著，与CK1和CK2相比分别增加了3.5%和13.5%。可溶性糖含量较CK2无显著差异，但较CK1相比则增加了106.6%。可溶性蛋白含量也有所提高，分别比CK1和CK2高出了16.4%和3.9%。丙二醛（MDA）含量及超氧阴离子（O2-）水平在引发后均有所下降，分别比CK1和CK2降低了39.0%和11.8%，以及15.5%和12.1%。综上所述，40 mmol/L CaCl2引发12 h处理能有效提升菜豆的光合效率，改善多项生理生化指标，从而促进菜豆生长。研究为CaCl2种子引发在促进菜豆幼苗生长和产生的生理生化效应上提供实际参考价值。

关键词: 菜豆, 种子, 引发, 光合参数, 生理生化指标, 氯化钙

Abstract:

The aim was to study the effects of CaCl2 initiation on bean seedlings, and to provide practical reference value for promoting the growth and the physiological and biochemical effects produced by CaCl2 seedlings initiation. The bean variety ‘Jiangjun’ was used as the experimental material, and 40 mmol/L CaCl2 was used to initiate the bean seeds for 12 h. The results showed that 12 h after CaCl2 induction, the dry weight of beans seedlings was increased by 38.8% and 19.2% compared with dry seed control (CK1) and distilled water control (CK2), respectively. The net photosynthetic rates were increased by 25.8% and 16.4%, respectively. The total activity of (α+β) amylase was increased by 79.9% and 15.3%, respectively. SOD, POD and CAT showed an increasing trend compared with the two controls, and CAT was the most significant, which was increased by 3.5% and 13.5% compared with CK1 and CK2, respectively. The soluble sugar content was not significantly different from CK2, but it was 106.6% higher than CK1. Soluble protein was increased by 16.4% and 3.9%, respectively. Compared with CK1 and CK2, MDA was decreased by 39.0% and 11.8%, and O2- was decreased by 15.5% and 12.1%, respectively. After initiation of 40 mmol/L CaCl2 for 12 h, the photosynthetic efficiency was effectively improved, and various physiological and biochemical indexes were improved, thus it promoted the growth of bean.

Key words: common bean, seed, initiator, photosynthetic parameters, physiological and biochemical index, CaCl2