‘航育1号’芦竹组培快繁体系的建立

doi:10.11924/j.issn.1000-6850.casb2024-0704

摘要/Abstract

摘要： 为建立芦竹优良品种‘航育1号’的高效组培快繁体系，以3年生植株茎段为外植体，系统探究取样时间及植物生长调节剂组合对再生体系的影响。结果表明，最佳采样时间为7—8月，此时田间植株处于快速生长期，外植体经75%酒精30 s+0.1% HgCl₂ 15~20 min消毒处理后成活率达90%；腋芽诱导最优培养基为MS+6-BA 4 mg/L+IBA 1 mg/L，诱导率92.66%；增殖培养基采用MS+6-BA 7 mg/L+IBA 1 mg/L，增殖系数达3.51；生根培养基为1/2MS+NAA 0.2 mg/L，生根率97.36%。移栽时选择生根7~10 d的瓶苗，成活率达92.22%，当年4月底定植后株高可达3~3.5 m，分蘖数30~50个。本研究通过优化外植体处理、激素配比及炼苗移栽等关键技术，建立了完整的‘航育1号’芦竹组培快繁技术体系，为其规模化生产提供了理论依据与技术支撑。

关键词: 芦竹, ‘航育1号’, 组织培养, 快繁体系, 茎段, 生长调节剂, 规模化生产

Abstract:

In order to accelerate propagation of elite variety of Arundo donax ‘Hangyu No.1’, and establish an efficient regeneration system, the stem segments of 3-year-old plants were used as explants to systematically explore the effects of sampling time and plant growth regulator combinations on the regeneration system. The results revealed that the best sampling time was in July and August, and the field plants were in a rapid growth period. The explant sterilization used 75% ethanol for 30 seconds and then 0.1% HgCl₂ for 15-20 min, resulting in a survival rate of 90% approximately. The medium for axillary bud induction was MS+ 6-BA 4 mg/L+ IBA 1 mg/L, 92.66% buds developed obviously. Bud proliferation medium was MS+ 6-BA 7 mg/L+IBA 1 mg/L, resulting in a proliferation coefficient of 3.51. The rooting medium was 1/2MS+ NAA 0.2 mg/L, and the rooting rate reached 97.36%. The best time for plant transfer to ex vitro conditions was 7-10 days after rooting. The survival rate was 92.22% in all the transferred plants. The plants were further moved to the field at the end of April and then under the standard field management. In November of the same year, the plant grew up to 3-3.5 m high with 30-50 tillers. This research provides solid technical support for large-scale production of ‘Hangyu No.1’.

Key words: Arundo donax, ‘Hangyu No.1’, tissue culture, efficient propagation system, stem, plant growth regulator, large-scale production

李珊珊, 刘宝平, 李亚平, 李晶炤, 晏武, 刘次次, 王艺. ‘航育1号’芦竹组培快繁体系的建立[J]. 中国农学通报, 2025, 41(16): 45-50.

LI Shanshan, LIU Baoping, LI Yaping, LI Jingzhao, YAN Wu, LIU Cici, WANG Yi. An Efficient Micro Propagation System for Arundo donax ‘Hangyu No.1’[J]. Chinese Agricultural Science Bulletin, 2025, 41(16): 45-50.

图/表 6

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取样时间	消毒时间/min	萌发率/%	污染率/%	死亡率/%
6月	5	56.92	40.87	2.21
	10	63.88	32.39	3.73
	15	48.52	25.77	25.71
	20	42.64	13.99	43.37
7月	5	63.19	35.68	1.13
	10	82.87	15.51	1.62
	15	90.25	7.13	2.62
	20	84.79	2.46	12.75
8月	10	71.55	28.22	0.23
	15	86.64	13.25	0.11
	20	90.53	6.24	3.23
	25	83.21	0.99	15.8
9月	10	63.96	34.71	1.33
	15	71.84	27.81	0.35
	20	79.34	19.26	1.4
	25	72.34	14.25	13.41

取样时间	消毒时间/min	萌发率/%	污染率/%	死亡率/%
6月	5	56.92	40.87	2.21
	10	63.88	32.39	3.73
	15	48.52	25.77	25.71
	20	42.64	13.99	43.37
7月	5	63.19	35.68	1.13
	10	82.87	15.51	1.62
	15	90.25	7.13	2.62
	20	84.79	2.46	12.75
8月	10	71.55	28.22	0.23
	15	86.64	13.25	0.11
	20	90.53	6.24	3.23
	25	83.21	0.99	15.8
9月	10	63.96	34.71	1.33
	15	71.84	27.81	0.35
	20	79.34	19.26	1.4
	25	72.34	14.25	13.41

试验序号	6-BA/(mg/L)	IBA/(mg/L)	腋芽诱导率/%
A1	0	0	62.90±0.59j
A2	2	0.5	83.19±0.47d
A3	2	1	79.68±0.55e
A4	2	1.5	70.04±0.52h
A5	4	0.5	86.21±0.57c
A6	4	1	92.66±0.89a
A7	4	1.5	89.00±0.52b
A8	6	0.5	85.99±0.64c
A9	6	1	75.84±0.7f
A10	6	1.5	72.89±0.56g
A11	8	0.5	65.96±0.55i
A12	8	1	76.00±0.55f
A13	8	1.5	85.96±0.59c
k₁	77.63	80.34
k₂	89.29	81.05
k₃	78.24	79.47
k₄	75.97
R	13.32	1.58
F	27.18^**	2.42

试验序号	6-BA/(mg/L)	IBA/(mg/L)	腋芽诱导率/%
A1	0	0	62.90±0.59j
A2	2	0.5	83.19±0.47d
A3	2	1	79.68±0.55e
A4	2	1.5	70.04±0.52h
A5	4	0.5	86.21±0.57c
A6	4	1	92.66±0.89a
A7	4	1.5	89.00±0.52b
A8	6	0.5	85.99±0.64c
A9	6	1	75.84±0.7f
A10	6	1.5	72.89±0.56g
A11	8	0.5	65.96±0.55i
A12	8	1	76.00±0.55f
A13	8	1.5	85.96±0.59c
k₁	77.63	80.34
k₂	89.29	81.05
k₃	78.24	79.47
k₄	75.97
R	13.32	1.58
F	27.18^**	2.42

试验序号	6-BA/(mg/L)	IBA/(mg/L)	增殖系数
B1	4	0.5	2.30±0.05h
B2	4	1	2.41±0.06g
B3	4	1.5	2.60±0.16f
B4	7	0.5	3.20±0.03b
B5	7	1	3.51±0.04a
B6	7	1.5	3.10±0.06c
B7	9	0.5	2.99±0.25d
B8	9	1	2.61±0.08f
B9	9	1.5	2.82±0.16e
k₁	2.43	2.83
k₂	3.27	2.84
k₃	2.81	2.84
R	0.84	0.01
F	125.06^**	13.90