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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (5): 39-43.doi: 10.11924/j.issn.1000-6850.casb2024-0103

• 生物科学 • 上一篇    下一篇

蚕豆籽粒蛋白含量遗传分析

陈志凯1(), 侯万伟1,2,3(), 周仙莉1, 陶伟1, 郑栋1, 张金发1   

  1. 1 青海大学，西宁 810016
    2 青海省农林科学院，西宁 810016
    3 农业农村部作物基因资源与种质创制青海科学观测实验站/国家农作物种质资源复份库，西宁 810016
  • 收稿日期:2024-02-20 修回日期:2024-05-15 出版日期:2025-02-15 发布日期:2025-02-11
  • 通讯作者:
    侯万伟，男，1982年出生，青海湟源人，副研究员，博士，主要从事作物遗传育种方面的研究。通信地址：810016 青海省西宁市城北区宁大路253号，E-mail：。
  • 作者简介:

    陈志凯，男，1997年出生，山西大同人，硕士研究生，研究方向：作物遗传育种研究。通信地址：810016 青海省西宁市城北区青海大学农科院，E-mail：。

  • 基金资助:
    “现代农业产业技术体系国家食用豆产业技术体系”(CARS-08-G06); “青海省高端人才千人计划”(培养拔尖2022)

Genetic Analysis of Protein Content in Vicia faba L. Seeds

CHEN Zhikai1(), HOU Wanwei1,2,3(), ZHOU Xianli1, TAO Wei1, ZHENG Dong1, ZHANG Jinfa1   

  1. 1 Qinghai University, Xining 810016
    2 Qinghai Academy of Agriculture and Forestry Sciences, Xining 810016
    3 Qinghai Scientific Observation and Experimental Station of Crop Gene Resources and Germplasm Creation, Ministry of Agriculture and Rural Affairs/National Crop Germplasm Resources Duplicate Library, Xining 810016
  • Received:2024-02-20 Revised:2024-05-15 Published:2025-02-15 Online:2025-02-11

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摘要：

为了保障温带地区优质植物蛋白的供给，加快选育高蛋白的蚕豆品种，对蚕豆蛋白含量遗传规律进行分析。以高蛋白含量材料‘羊眼豆’(H0001860)为父本，低蛋白含量材料‘大粒豆’(H0004518)为母本构建F2群体，利用杜马斯燃烧定氮法和主基因+多基因混合模型对蚕豆蛋白含量进行遗传分析。蚕豆蛋白含量的最优遗传模型为2MG-EA（2对等加性主基因）模型，2对等加性主基因模型的加性效应值da(d)为1.7238，加性效应表现为正向效应。主基因的遗传方差为2.4817，主基因遗传率较高(62.0286%)。蚕豆蛋白含量的最优遗传模型为2对等加性主基因模型，蚕豆蛋白含量的遗传改良应该在早期分离世代进行。

关键词: 蚕豆, 蛋白质含量, 杜马斯燃烧定氮法, 遗传模型, 主基因+多基因模型

Abstract:

In order to guarantee the supply of high-quality plant protein in temperate regions and accelerate the selection and breeding of high-protein faba bean varieties, the genetic law of protein content of faba bean was analyzed. An F2 population was constructed with the high-protein content material 'Sheep's Eye Bean' (H0001860) as the father and the low-protein content material 'Big Grain Bean' (H0004518) as the mother, and the genetic analysis of faba bean protein content was carried out by using the Dumas combustion nitrogen determination method and the main gene+multigene hybrid model. The optimal genetic model for protein content of faba bean was 2MG-EA (2 pairs of equal additive principal genes) model, and the additive effect value da(d) of the 2 pairs of equal additive principal genes model was 1.7238, and the additive effect showed a positive effect. The genetic variance of the main gene was 2.4817, and the heritability of the main gene was high (62.0286%). The optimum genetic model for protein content of faba bean is 2 pairs of equal additive main gene model, and genetic improvement of protein content of faba bean should be carried out in early segregating generations.

Key words: Vicia faba L., protein content, dumas combustion nitrogen determination method, genetic model, main gene+multigene hybrid model