壳寡糖及微生物代谢产物在农业生产中的应用

doi:10.11924/j.issn.1000-6850.casb2024-0398

中国农学通报 ›› 2025, Vol. 41 ›› Issue (8): 83-89.doi: 10.11924/j.issn.1000-6850.casb2024-0398

• 资源·环境·生态·土壤·气象 • 上一篇下一篇

壳寡糖及微生物代谢产物在农业生产中的应用

杨莹¹(), 樊丽², 杨志超¹, 曹鑫博¹, 葛菁萍¹()

¹ 黑龙江大学生命科学学院，农业微生物技术教育部工程研究中心，黑龙江省寒区植物基因与生物发酵重点实验室，黑龙江省普通高校微生物重点实验室，哈尔滨 150080
² 齐齐哈尔市药品检验中心，黑龙江齐齐哈尔 161000

收稿日期:2024-06-21 修回日期:2024-09-15 出版日期:2025-03-14 发布日期:2025-03-14
通讯作者:
葛菁萍，女，1972年出生，黑龙江齐齐哈尔人，教授，博士，研究方向：微生物资源挖掘与利用。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学224信箱，Tel：0451-86609016，E-mail：gejingping@126.com。
作者简介:
杨莹，女，2001年出生，黑龙江伊春人，硕士研究生，研究方向：微生物资源挖掘与利用。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号，Tel：0451-86609016，E-mail：yangying010427@126.com。
基金资助:
黑龙江省高校基本业务经费“群体感应介导下重组根圈细菌菌群对土壤磷养分循环的调控机制”(2023-KYYWF-1448); 黑龙江省生态环境厅生态环境保护科研项目“人工重组细菌菌群对黑土磷障碍的解控机制”(HST20TR004); 黑龙江省自然科学基金联合培育项目“群体感应对寒地黑土遗留有机磷的矿化及迁移驱动机制研究”(PL2024D015)

Application of COS and Microbial Metabolites in Agricultural Production

YANG Ying¹(), FAN Li², YANG Zhichao¹, CAO Xinbo¹, GE Jingping¹()

¹ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education / Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region / Key Laboratory of Microbiology, College of Heilongjiang Province / School of Life Sciences, Heilongjiang University, Harbin 150080
² Qiqihar Institute for Drug Control, Qiqihar, Heilongjiang 161000

Received:2024-06-21 Revised:2024-09-15 Published:2025-03-14 Online:2025-03-14

摘要/Abstract

摘要：

本研究综合评估了壳寡糖(Chitosan oligosaccharide, COS)的制备技术，并深入分析了COS和微生物代谢产物对植物生长、作物品质提升及病虫害管理的影响，旨在增强农业生产效率和推动环境的可持续性。通过实验研究发现，COS和微生物代谢产物在提高农业产量和维持环境稳定方面具有重要作用。针对由自然和人为活动引起的气候和生态系统变化对粮食安全构成的挑战，我们提出未来开展关于COS等有机纳米颗粒以及微生物代谢产物活性的深入研究，并强调加强发展新兴肥料研究的必要性，以期为农业生产的可持续发展提供科学依据和技术支持。

关键词: 壳寡糖, 微生物代谢产物, 应用, 生物防治, 促进植物生长发育, 作物品质, 病虫害管理, 可持续发展

Abstract:

This study comprehensively evaluated the preparation techniques of chitosan oligosaccharide (COS) and conducted an in-depth analysis of the effects of COS and microbial metabolites on plant growth, crop quality enhancement, and pest management, aiming to enhance agricultural production efficiency and promote environmental sustainability. The results revealed the importance of COS and microbial metabolites in improving agricultural yield and maintaining environmental stability. In response to the challenges posed to food security by climate and ecosystem changes caused by natural and human activities, we propose that future research should focus on the activities of organic nanoparticles such as COS and microbial metabolites, and emphasize the necessity of advancing emerging fertilizer research to provide scientific basis and technical support for the sustainable development of agricultural production.

Key words: chitosan oligosaccharide (COS), microbial metabolites, application, biological control, promote plant growth and development, crop quality, pest management, sustainable development

杨莹, 樊丽, 杨志超, 曹鑫博, 葛菁萍. 壳寡糖及微生物代谢产物在农业生产中的应用[J]. 中国农学通报, 2025, 41(8): 83-89.

YANG Ying, FAN Li, YANG Zhichao, CAO Xinbo, GE Jingping. Application of COS and Microbial Metabolites in Agricultural Production[J]. Chinese Agricultural Science Bulletin, 2025, 41(8): 83-89.

图/表 2

参考文献 66

[1]	ABBAS M, RIBEIRO P F, SANTOS J L. Farming system change under different climate scenarios and its impact on food security: an analytical framework to inform adaptation policy in developing countries[J]. Mitigation and adaptation strategies for global change, 2023, 28(8):43.
[2]	KASTNER T, CHAUDHARY A, GINGRICH S, et al. Global agricultural trade and land system sustainability: Implications for ecosystem carbon storage, biodiversity, and human nutrition[J]. One earth, 2021, 4(10):1425-1443.
[3]	KHAN S, HANJRA M A. Footprints of water and energy inputs in food production - Global perspectives[J]. Food policy, 2009, 34(2):130-140.
[4]	GAO X, LI C, ZHANG M, et al. Controlled release urea improved the nitrogen use efficiency, yield and quality of potato (Solanum tuberosum L.) on silt loamy soil[J]. Field crops research, 2015,181:60-68.
[5]	MOORE J A M, ABRAHAM P E, MICHENER JOSHUA K, et al. Ecosystem consequences of introducing plant growth promoting rhizobacteria to managed systems and potential legacy effects[J]. New phytologist, 2022, 234(6):1914-1918.
[6]	JIANG Y, YUE Y, WANG Z, et al. Plant biostimulant as an environmentally friendly alternative to modern agriculture[J]. Journal of agricultural and food chemistry, 2024, 72(10):5107-5121. doi: 10.1021/acs.jafc.3c09074 pmid: 38428019
[7]	MUANPRASAT C, CHATSUDTHIPONG V. Chitosan oligosaccharide: Biological activities and potential therapeutic applications[J]. Pharmacology & therapeutics, 2017,170:80-97.
[8]	MUKHTAR AHMED K B, KHAN M M A, SIDDIQUI H, et al. Chitosan and its oligosaccharides, a promising option for sustainable crop production- a review[J]. Carbohydrate polymers, 2020,1:227:115331.
[9]	LIAQAT F, ELTEM R. Chitooligosaccharides and their biological activities: A comprehensive review[J]. Carbohydrate polymers, 2018,184:243-259.
[10]	TROMBOTTO S, LADAVIERE C, DELOLME F, et al. Chemical preparation and structural characterization of a homogeneous series of chitin/chitosan oligomers[J]. Biomacromolecules, 2008, 9(7):1731-1738. doi: 10.1021/bm800157x pmid: 18547106
[11]	ZHISHEN J D S. Eeffect of reaction temperature and reaction time on the preparation of low-molecular-weight chitosan using phosphoric acid[J]. Carbohydrate polymers, 2002,49:393-396.
[12]	TISHCHENKO G, ŠIMŮNEK J, BRUS J, et al. Low-molecular-weight chitosans: Preparation and characterization[J]. Carbohydrate polymers, 2011, 86(2):1077-1081.
[13]	XIA Z, WU S, CHEN J. Preparation of water soluble chitosan by hydrolysis using hydrogen peroxide[J]. International journal of biological macromolecules, 2013,59:242-245.
[14]	MITTAL A, SINGH A, BUATONG J, et al. Chitooligosaccharide and its derivatives: potential candidates as food additives and bioactive components[J]. Foods, 2023, 12(20):3854-3882.
[15]	POPA-NITA S, LUCAS J-M, LADAVIERE C, et al. Mechanisms involved during the ultrasonically induced depolymerization of chitosan: Characterization and control[J]. Biomacromolecules, 2009, 10(5):1203-1211.
[16]	TERáN HILARES R, DOS SANTOS J G, SHIGUEMATSU N B, et al. Low-pressure homogenization of tomato juice using hydrodynamic cavitation technology: Effects on physical properties and stability of bioactive compounds[J]. Ultrasonics sonochemistry, 2019,54:192-197.
[17]	WU Y, HUANG Y, ZHOU Y, et al. Degradation of chitosan by swirling cavitation[J]. Innovative food science & emerging technologies, 2014,23:188-193.
[18]	VISHU KUMAR A. Low molecular weight chitosans: preparation with the aid of papain and characterization[J]. Biochimica et biophysica acta (BBA) - general subjects, 2004, 1670(2):137-146.
[19]	CHENG C Y, CHANG C H, WU Y J, et al. Exploration of glycosyl hydrolase family 75, a chitosanase from Aspergillus fumigatus[J]. Journal of biological chemistry, 2006, 281(6):3137-3144.
[20]	POSHINA D N, RAIK S V, POSHIN A N, et al. Accessibility of chitin and chitosan in enzymatic hydrolysis: A review[J]. Polymer degradation and stability, 2018,156:269-278.
[21]	WU S. Preparation of chitooligosaccharides from Clanis bilineata larvae skin and their antibacterial activity[J]. International journal of biological macromolecules, 2012, 51(5):1147-1150.
[22]	HAMER S N, CORD-LANDWEHR S, BIARNéS X, et al. Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases[J]. Scientific reports, 2015,3:5:8716.
[23]	HEMBACH L, CORD-LANDWEHR S, MOERSCHBACHER B M. Enzymatic production of all fourteen partially acetylated chitosan tetramers using different chitin deacetylases acting in forward or reverse mode[J]. Scientific reports, 2017, 7(1):17692. doi: 10.1038/s41598-017-17950-6 pmid: 29255209
[24]	CAI Q, GU Z, CHEN Y, et al. Degradation of chitosan by an electrochemical process[J]. Carbohydrate polymers, 2010, 79(3):783-785.
[25]	GU Z, CAI Q, LIU Y, et al. Electrochemical degradation of chitosan using Ti/Sb-SnO₂ Electrode[J]. Journal of polymers and the environment, 2012, 21(2):479-486.
[26]	JIA X, MENG Q, ZENG H, et al. Chitosan oligosaccharide induces resistance to Tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signalling pathway[J]. Scientific reports, 2016,18:6:26144.
[27]	ZHANG H, ZHAO X, YANG J, et al. Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide[J]. Plant cell reports, 2011, 30(6):1153-1162. doi: 10.1007/s00299-011-1024-z pmid: 21336582
[28]	YANG A, YU L, CHEN Z, et al. Label-free quantitative proteomic analysis of chitosan oligosaccharide-treated rice infected with southern rice black-streaked dwarf virus[J]. Viruses, 2017, 9(5):115.
[29]	COBOS R, MATEOS R M, ÁLVAREZ-PéREZ J M, et al. Effectiveness of natural antifungal compounds in controlling infection by grapevine trunk disease pathogens through pruning wounds[J]. Applied and environmental microbiology, 2015, 81(18):6474-6483. doi: 10.1128/AEM.01818-15 pmid: 26162882
[30]	KIM S W, PARK J K, LEE C H, et al. Comparison of the antimicrobial properties of chitosan oligosaccharides (COS) and EDTA against Fusarium fujikuroi causing rice bakanae disease[J]. Current microbiology, 2016, 72(4):496-502.
[31]	SUN G, YANG Q, ZHANG A, et al. Synergistic effect of the combined bio-fungicides ε-poly- l -lysine and chitooligosaccharide in controlling grey mould ( Botrytis cinerea ) in tomatoes[J]. International journal of food microbiology, 2018,276:46-53.
[32]	YIN H, FRETTé X C, CHRISTENSEN L P, et al. Chitosan oligosaccharides promote the content of polyphenols in greek oregano (Origanum vulgare ssp. hirtum)[J]. Journal of agricultural and food chemistry, 2011, 60(1):136-143.
[33]	GUO W, YIN H, YE Z, et al. A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method[J]. Carbohydrate polymers, 2012, 90(1):491-495. doi: 10.1016/j.carbpol.2012.05.070 pmid: 24751069
[34]	GOUGH C, CULLIMORE J. Lipo-chitooligosaccharide signaling in edosymbiotic plant-microbe interactions[J]. Molecular plant-microbe interactions, 2011, 24(8):867-878.
[35]	TANAKA K, CHO S-H, LEE H, et al. Effect of lipo-chitooligosaccharide on early growth of C4 grass seedlings[J]. Journal of experimental botany, 2015, 66(19):5727-5738. doi: 10.1093/jxb/erv260 pmid: 26049159
[36]	HE Y, BOSE S, WANG W, et al. Pre-harvest treatment of chitosan oligosaccharides improved strawberry fruit quality[J]. International journal of molecular sciences, 2018, 19(8):2194.
[37]	GUO X, YU Z, ZHANG M, et al. Enhancing the production of phenolic compounds during barley germination by using chitooligosaccharides to improve the antioxidant capacity of malt[J]. Biotechnology letters, 2018, 40(9-10):1335-1341. doi: 10.1007/s10529-018-2582-8 pmid: 29876794
[38]	LAN W, WANG W, YU Z, et al. Enhanced germination of barley (Hordeum vulgare L.) using chitooligosaccharide as an elicitor in seed priming to improve malt quality[J]. Biotechnology letters, 2016, 38(11):1935-1940.
[39]	ABDEL LATEF A A H, ABU ALHMAD M F, KORDROSTAMI M, et al. Inoculation with Azospirillum lipoferum or Azotobacter chroococcum reinforces maize growth by improving physiological activities under saline conditions[J]. Journal of plant growth regulation, 2020, 39(3):1293-1306.
[40]	OZIMEK E, JAROSZUK-ŚCISEŁ J, BOHACZ J, et al. Synthesis of indoleacetic ccid, gibberellic acid and ACC-deaminase by Mortierella strains promote winter wheat seedlings growth under different conditions[J]. International journal of molecular sciences, 2018, 19(10):3218.
[41]	PARAĐIKOVIĆ N, TEKLIĆ T, ZELJKOVIĆ S, et al. Biostimulants research in some horticultural plant species—a review[J]. Food and energy security, 2018, 8(2):e00162.
[42]	KOUR D, RANA K L, YADAV A N, et al. Microbial biofertilizers: bioresources and eco-friendly technologies for agricultural and environmental sustainability[J]. Biocatalysis and agricultural biotechnology, 2020,23:101487.
[43]	RADHAKRISHNAN R, HASHEM A, ABD_ALLAH E F. Bacillus: a biological tool for crop improvement through bio-molecular changes in adverse environments[J]. Frontiers in physiology, 2017,8:667.
[44]	DÍAZ-RUEDA P, MORALES DE LOS RÍOS L, ROMERO L C, et al. Old poisons, new signaling molecules: the case of hydrogen cyanide[J]. Journal of experimental botany, 2023, 74(19):6040-6051.
[45]	LLORENTE B E, ALASIA M A, LARRABURU E E. Biofertilization with Azospirillum brasilense improves in vitro culture of Handroanthus ochraceus, a forestry, ornamental and medicinal plant[J]. New biotechnology, 2016, 33(1):32-40.
[46]	ORTIZ-CASTRO R, CAMPOS-GARCíA J, LóPEZ-BUCIO J. Pseudomonas putida and Pseudomonas fluorescens influence Arabidopsis root system architecture through an auxin response mediated by bioactive cyclodipeptides[J]. Journal of plant growth regulation, 2019, 39(1):254-265.
[47]	HELLEQUIN E, MONARD C, CHORIN M, et al. Responses of active soil microorganisms facing to a soil biostimulant input compared to plant legacy effects[J]. Scientific reports, 2020, 10(1):13727. doi: 10.1038/s41598-020-70695-7 pmid: 32792675
[48]	CHATTOPADHYAY A, PUROHIT J, TIWARI K K, et al. Targeting transcription factors for plant disease resistance: shifting paradigm[J]. Current science, 2019, 117(10):1598-1607.
[49]	TANG C, XU Q, ZHAO M, et al. Understanding the lifestyles and pathogenicity mechanisms of obligate biotrophic fungi in wheat: the emerging genomics era[J]. The crop journal, 2018, 6(1):60-67.
[50]	TELI B, PUROHIT J, RASHID M M, et al. Omics insight on Fusarium Head Blight of wheat for translational research perspective[J]. Current genomics, 2020, 21(6):411-428.
[51]	SUSIČ N, JANEŽIČ S, RUPNIK M, et al. Whole genome sequencing and comparative genomics of two nematicidal Bacillus strains reveals a wide range of possible virulence factors[J]. G3 genes genomes genetics, 2020, 10(3):881-890.
[52]	MARCHE M G, CAMIOLO S, PORCEDDU A, et al. Survey of Brevibacillus laterosporus insecticidal protein genes and virulence factors[J]. Journal of invertebrate pathology, 2018,155:38-43.
[53]	BLAINSKI J M L, DA ROCHA NETO A C, SCHIMIDT E C, et al. Exopolysaccharides from Lactobacillus plantarum induce biochemical and physiological alterations in tomato plant against bacterial spot[J]. Applied microbiology and biotechnology, 2018, 102(11):4741-4753.
[54]	BENEDUZI A, AMBROSINI A, PASSAGLIA L M P. Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents[J]. Genetics and molecular biology, 2012, 35(4):1044-1051.
[55]	LU X, LIU S-F, YUE L, et al. Epsc involved in the encoding of exopolysaccharides produced by Bacillus amyloliquefaciens FZB42 act to boost the drought tolerance of Arabidopsis thaliana[J]. International journal of molecular sciences, 2018, 19(12):3795.
[56]	O'BRIEN S, HODGSON D J, BUCKLING A. Social evolution of toxic metal bioremediation in Pseudomonas aeruginosa[J]. Proceedings of the royal society b-biological sciences, 2014, 281(1787):20240858.
[57]	WANG Q, XIONG D, ZHAO P, et al. Effect of applying an arsenic-resistant and plant growth-promoting rhizobacterium to enhance soil arsenic phytoremediation by Populus deltoides LH05-17[J]. Journal of applied microbiology, 2011, 111(5):1065-1074.
[58]	SULTANA S, ALAM S, KARIM M M. Screening of siderophore-producing salt-tolerant rhizobacteria suitable for supporting plant growth in saline soils with iron limitation[J]. Journal of agriculture and food research, 2021,4:100150.
[59]	GAMALERO E, GLICK B R. Bacterial modulation of plant ethylene levels[J]. Plant physiology, 2015, 169(1):13-22. doi: 10.1104/pp.15.00284 pmid: 25897004
[60]	VISWANATHAN V K, RAJARAM MANOHARAN S R, SUBRAMANIAN S, et al. Nanotechnology in spine surgery: a current update and critical review of the literature[J]. World neurosurgery, 2019,123:142-155.
[61]	BUEHLER M J, YUNG Y C. Deformation and failure of protein materials in physiologically extreme conditions and disease[J]. Nature materials, 2009, 8(3):175-188. doi: 10.1038/nmat2387 pmid: 19229265
[62]	SABERI-RISE R, MORADI-POUR M. The effect of Bacillus subtilis Vru1 encapsulated in alginate- bentonite coating enriched with titanium nanoparticles against Rhizoctonia solani on bean[J]. International journal of biological macromolecules, 2020,152:1089-1097.
[63]	ZAHRA Z, HABIB Z, HYUN H, et al. Overview on recent developments in the design, application, and impacts of nanofertilizers in agriculture[J]. Sustainability, 2022, 14(15):9397.
[64]	AKHTAR N, ILYAS N. Role of nanosilicab to boost the activities of metabolites in Triticum aestivum facing drought stress[J]. Plant and soil, 2022, 477(1-2):99-115.
[65]	ELIASPOUR S, SEYED SHARIFI R, SHIRKHANI A, et al. Effects of biofertilizers and iron nano-oxide on maize yield and physiological properties under optimal irrigation and drought stress conditions[J]. Food science & nutrition, 2020, 8(11):5985-5998.
[66]	ZAIM N S H B H, TAN H L, RAHMAN S M A, et al. Recent advances in seed coating treatment using nanoparticles and nanofibers for enhanced seed germination and protection[J]. Journal of plant growth regulation, 2023, 42(12):7374-7402.

COS制备方法	优点	缺点
化学方法	操作简单	产品分离纯化困难
物理方法	易纯化，污染小	产率低
酶解法	易获得，无需额外修饰	成本高且可用性低
电化学法	操作方便，无污染	电极寿命短，容易失效

COS制备方法	优点	缺点
化学方法	操作简单	产品分离纯化困难
物理方法	易纯化，污染小	产率低
酶解法	易获得，无需额外修饰	成本高且可用性低
电化学法	操作方便，无污染	电极寿命短，容易失效

壳寡糖及微生物代谢产物在农业生产中的应用

Application of COS and Microbial Metabolites in Agricultural Production

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 66

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	梁辉, 邓全, 刘国, 陈河竹, 马鹏, 李斌, 刘东阳, 余佳敏, 江连强, 蒲德强. 密度对十斑大瓢虫不同虫态生存的影响[J]. 中国农学通报, 2025, 41(8): 105-110.
[2]	吴陈阳, 袁野, 陈晓鹏, 林伟坤, 林喜强, 郑怀平, 马洪雨. 多营养层级综合养殖模式的应用现状及展望[J]. 中国农学通报, 2025, 41(7): 154-164.
[3]	刘威, 蔡卫佳, 王昊, 罗桂杰, 刘旭. 食叶草研究进展[J]. 中国农学通报, 2025, 41(3): 36-41.
[4]	刘燕, 韩伟. 不同药剂处理对马铃薯晚疫病的田间防治效果[J]. 中国农学通报, 2025, 41(2): 117-122.
[5]	董猛, 宋大鹏, 王鲲鹏, 丁仕波, 王莹莹, 李海鹏, 杨峰山, 付海燕. L-茶氨酸合成途径及应用展望[J]. 中国农学通报, 2025, 41(2): 140-148.
[6]	李勇, 赖旭辉, 李春梅, 姚琼, 刘凯. 匙羹藤的应用及研究进展[J]. 中国农学通报, 2025, 41(1): 89-95.
[7]	杨义, 赵守祺, 葛菁萍, 宋刚, 杜仁鹏. 微生物胞外多糖在环境中的应用[J]. 中国农学通报, 2024, 40(9): 66-74.
[8]	卫甜, 杨倩, 刘怀阿, 朱锦磊, 吕敏. 高地芽孢杆菌对稻瘟病的防治及促生作用[J]. 中国农学通报, 2024, 40(7): 123-128.
[9]	魏莲, 刘虹伶, 伍兴隆, 陈河竹, 彭应力, 肖科军, 蔡鹏, 房超, 李跃建, 蒲德强. 十斑大瓢虫对豆蚜的捕食特性研究[J]. 中国农学通报, 2024, 40(5): 105-109.
[10]	符百文, 许炼, 郑梅霞, 朱育菁. 苏云金芽胞杆菌Cry毒素的研究现状[J]. 中国农学通报, 2024, 40(5): 88-96.
[11]	林接英, 崔一平, 黄峰, 牟桂萍, 岳茂峰, 宋晓兵. 柑橘黄龙病防治技术最新研究进展[J]. 中国农学通报, 2024, 40(36): 126-131.
[12]	杨洋, 赵官涛, 王露, 王琼, 朱珍花, 张佩, 何玉娇, 赵长增. 昆虫对Bt作物的抗性机制以及治理策略的研究进展[J]. 中国农学通报, 2024, 40(33): 141-149.
[13]	刘虹伶, 何蓉, 余佳敏, 邓全, 刘东阳, 李思翰, 张培旭, 雍艳萍, 伍兴隆, 肖科军, 蒲德强. 氯化胆碱对七星瓢虫成虫生物学特性的影响[J]. 中国农学通报, 2024, 40(33): 150-156.
[14]	张红艳, 吴影, 古绍彬. 益生菌发酵中药在动物养殖上的应用研究[J]. 中国农学通报, 2024, 40(33): 157-164.
[15]	王丽娜, 王迪, 任翠梅, 顾鑫, 张宏宇, 李娜, 齐国超, 冯鹏. 土壤改良剂的应用现状[J]. 中国农学通报, 2024, 40(30): 84-88.