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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (11): 39-49.doi: 10.11924/j.issn.1000-6850.casb2025-0684

• 生物科学 • 上一篇    下一篇

桔绿木霉HT-1分生孢子固体发酵优化及其对2种作物幼苗生长的影响

王姣姣1(), 李静1, 王红丽1,2, 赵婧1, 孙坤1, 陈大为1()   

  1. 1 西北师范大学生命科学学院， 兰州 730070
    2 甘肃省中医院， 兰州 730050
  • 收稿日期:2025-08-13 修回日期:2025-12-11 出版日期:2026-06-12 发布日期:2026-06-12
  • 通讯作者:
    陈大为，男，1993年出生，副教授，博士，研究方向：植物与微生物互作。通信地址：730070 甘肃省兰州市安宁区邱家湾27号 西北师范大学生命科学学院，Tel：0931-7971770，E-mail：。
  • 作者简介:

    王姣姣，女，2003年出生，甘肃陇南人，研究生在读，研究方向：微生物学。通信地址：730070 甘肃省兰州市安宁区邱家湾27号 西北师范大学生命科学学院，Tel：0931-7971770，E-mail：。

  • 基金资助:
    国家自然科学基金“掌叶大黄内生微生物组构建及其对有效成分合成的调控机制”(82360745); 甘肃省科技计划项目“农业废弃物资源化利用研发复合生物菌肥”(23CXGA0031); 西北师范大学青年教师科研能力提升计划“西藏沙棘对植物微生物组的性别特异性选择作用”(NWNU-LKQN2023-20)

Optimization of Solid-state Fermentation of Trichoderma citrinoviride HT-1 Conidia and Its Effect on Two Crop Seedlings Growth

WANG Jiaojiao1(), LI Jing1, WANG Hongli1,2, ZHAO Jing1, SUN Kun1, CHEN Dawei1()   

  1. 1 College of Life Sciences, Northwest Normal University, Lanzhou 730070
    2 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050
  • Received:2025-08-13 Revised:2025-12-11 Published:2026-06-12 Online:2026-06-12

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摘要：

针对当前木霉菌剂产业化中液体发酵成本高、孢子产量低、固体发酵工艺粗放等问题，为实现具有促生抑菌活性的桔绿木霉(Trichoderma citrinoviride)HT-1分生孢子的低成本高效生产。本研究以农业废弃物为固体发酵基质，采用单因素试验、正交优化与响应面法(RSM)，系统优化菌株HT-1的固体发酵基质配比与发酵条件，并通过盆栽试验验证其发酵产物对小麦、番茄幼苗的促生效果。结果表明：最优固体培养基质配比为玉米秸秆：麦麸：花生壳：棉籽壳=2:2:3:1；关键发酵参数为接种量14.22%，含水量48.89%，发酵时间13.27 d，此条件下菌株HT-1产孢量最高，达到了1.01×1011个/g；盆栽试验显示，发酵产物可显著提升小麦、番茄的株高、根长、鲜重与干重（P<0.05）。本研究建立了一套农业废弃物资源化、高产孢量、工艺稳定的桔绿木霉固体发酵技术，为桔绿木霉HT-1菌株的生物菌剂产业化开发与绿色农业应用提供了科学依据与技术支撑，后续可进一步开展制剂化、田间稳定性与促生机制研究。

关键词: 桔绿木霉, 固体发酵, 农业废弃物, 分生孢子, 响应面法, 促生作用

Abstract:

To address the challenges in the industrialization of Trichoderma-based biofungicides, including high liquid fermentation costs, low spore yields, and extensive solid-state fermentation processes, this study aimed to achieve cost-effective, high-efficiency conidia production of the plant growth-promoting and antagonistic strain Trichoderma citrinoviride HT-1. Using agricultural wastes as solid fermentation substrates, we systematically optimized the substrate composition and fermentation conditions through single-factor experiments, orthogonal array design, and response surface methodology. The growth-promoting effects of the fermented product were further evaluated on wheat and tomato seedlings in pot experiments. The optimal solid substrate formula was maize straw : wheat bran : peanut shell : cottonseed hull = 2 : 2 : 3 : 1. The key fermentation parameters were inoculum size (14.22%), moisture content (48.89%), and fermentation duration (13.27 days). Under these optimized conditions, strain HT-1 achieved a maximum spore yield of 1.01 × 1011 spores/g. Pot experiment results showed that the fermented product significantly increased plant height, root length, fresh weight, and dry weight of both wheat and tomato seedlings (P < 0.05). Collectively, this study establishes a stable, high-yield solid-state fermentation technology for T. citrinoviride that enables the valorization of agricultural wastes. These findings provide a scientific basis and technical support for the industrial development and green agricultural application of T. citrinoviride HT-1. Future research should focus on formulation development, field stability testing, and mechanistic studies of plant growth promotion.

Key words: Trichoderma citrinoviride, solid-state fermentation, agricultural waste, conidia, response surface methodology, growth-promoting effect

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