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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (12): 213-218.doi: 10.11924/j.issn.1000-6850.casb2025-0748

• 植物保护·农药 • 上一篇    

生姜储藏期黑霉病病原的分离鉴定与生物学特性研究

鲜赟曦(), 胡孜进, 黄小琴, 余垚颖, 邓越, 宋沛, 周西全, 刘勇, 张蕾, 张重梅, 杨潇湘()   

  1. 四川省农业科学院植物保护研究所/农业农村部西南作物有害生物综合治理重点实验室， 成都 610066
  • 收稿日期:2025-09-05 修回日期:2026-02-23 出版日期:2026-06-25 发布日期:2026-06-23
  • 通讯作者:
    杨潇湘，女，1987年出生，四川崇州人，副研究员，博士，主要从事植物病害生物防治研究。通信地址：610066 四川省成都市锦江区净居寺20号 四川省农业科学院植物保护研究所，Tel：028-84504089，E-mail：。
  • 作者简介:

    鲜赟曦，女，1995年出生，四川眉山人，助理研究员，主要从事植物病害绿色防控。通信地址：610066 四川省成都市锦江区净居寺20号 四川省农业科学院植物保护研究所，Tel：028-84504089，E-mail：。

  • 基金资助:
    四川省农业科学院科技成果中试熟化与示范转化工程项目“威远县连作重茬模式下生姜主要病害防治技术集成与示范”(2023ZSSFXD05-6)

Isolation and Identification of Pathogen Causing Black Mold Disease on Ginger During Storage and Study on Its Biological Characteristics

XIAN Yunxi(), HU Zijin, HUANG Xiaoqin, YU Yaoying, DENG Yue, SONG Pei, ZHOU Xiquan, LIU Yong, ZHANG Lei, ZHANG Zhongmei, YANG Xiaoxiang()   

  1. Institute of Plant Protection, Sichuan Academy of Agricultural Sciences / Key Laboratory of Integrated Pest Management in Southwest Agriculture Crops, Ministry of Agriculture and Rural Affairs, Chengdu 610066
  • Received:2025-09-05 Revised:2026-02-23 Published:2026-06-25 Online:2026-06-23

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摘要：

为明确四川地区生姜储藏期黑霉病的病原菌种类及其关键生物学特性，并为其绿色防控提供理论依据，本研究采用组织分离法获得了生姜黑霉病病原菌株CDSJ1。通过形态学观察、分子生物学鉴定（ITS序列分析）及柯赫氏法则验证，进行了病原鉴定；利用单因素实验，研究了温度、碳源、氮源及光照条件对菌株生长和产孢特性的影响。研究结果表明，引起生姜储藏期黑霉病的病原菌为黑曲霉(Aspergillus niger)，其最适生长温度为30℃，最适碳源为麦芽糖，最适氮源为胰蛋白胨，在全光照条件下产孢量最高。本研究系统揭示了四川生姜储藏期黑霉病的病原信息及其关键生物学特性，为制定生姜采后绿色防控技术提供了科学依据。

关键词: 生姜, 黑曲霉, 病原鉴定, 生物学特性, 储藏病害

Abstract:

This study aimed to identify the pathogen causing black mold on stored ginger in Sichuan and clarify its key biological characteristics, so as to provide a theoretical basis for green control strategies. The pathogenic strain CDSJ1 was obtained by tissue isolation, and identified based on morphological characteristics, molecular biology (ITS sequence analysis), and verification using Koch’s postulates. The effects of temperature, carbon source, nitrogen source, and light conditions on mycelial growth and sporulation were investigated through single-factor experiments. The pathogen was identified as Aspergillus niger. The optimal growth temperature was 30 ℃; the most suitable carbon and nitrogen sources were maltose and tryptone, respectively; and the highest sporulation level was observed under continuous light conditions. This study systematically identifies A. niger as the pathogen of black mold on stored ginger in Sichuan and elucidate its key biological characteristics, providing a scientific basis for the development of green postharvest disease management strategies.

Key words: ginger, Aspergillus niger, pathogen identification, biological characteristics, storage disease

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