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中国农学通报 ›› 2011, Vol. 27 ›› Issue (19): 228-233.

所属专题: 资源与环境 园艺

• 林学 园艺 园林 • 上一篇    下一篇

薄皮甜瓜种质资源遗传多样性的RAPD分析

智海英 马海龙 岳青   

  • 收稿日期:2011-01-26 修回日期:2011-03-08 出版日期:2011-08-05 发布日期:2011-08-05

RAPD Analysis of Genetic Diversity on Germplasm Resources of Oriental Melon

  • Received:2011-01-26 Revised:2011-03-08 Online:2011-08-05 Published:2011-08-05

摘要:

RAPD分子标记对来源不同的41份薄皮甜瓜种质资源进行了遗传多样性分析。11个随机引物共扩增出78条带,平均每个引物扩增的多态性带数为5.3条,多态性带百分率为74.4%。利用NTSYS-pc软件计算种质间的遗传距离,用UPGMA法进行聚类分析,将供试的41份种质可分为3个类群8组,用EIGEN方法进行主坐标分析,将其分为4个类群12组。两种分类方法结果基本一致,后者能更为直观地反映群体之间的关系。

关键词: 大豆蚜, 大豆蚜, 生物防治, 综述

Abstract:

The genetic diversity of oriental melon resources was analyzed with RAPD. 11 RAPD random primers produced 78 polymorphic bands, averaged 5.3 bands each primer pair. The average percentage of polymorphic bands was 74.4%. Genetic distance was calculated by the NTSYS-pc software. A dendrogram was constructed by using UPGMA based on genetic distance matrix. 41 accessions were clustered into three groups and eight subgroups based on the RAPD data by the method of clustering analysis, and four groups and twelve subgroups by the method of principal coordinates analysis. The results from these two methods were similar, but the result from the principal coordinate analysis was more informative.

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