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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (18): 233-236.

所属专题： 生物技术； 水稻

• 植物保护 农药 • 上一篇    下一篇

抗稻瘟病细胞突变体筛选技术的研究进展

李楠 陈巧玲 李亚娟 郭海滨 罗玉容   

  • 收稿日期:2011-03-25 修回日期:2011-05-26 出版日期:2011-07-25 发布日期:2011-07-25

Research and Development of a Technical System for Screening Rice (Oryza sativa L. subsp. indica) Somatic Mutant Resistance to Blast

  • Received:2011-03-25 Revised:2011-05-26 Online:2011-07-25 Published:2011-07-25

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摘要：

稻瘟病是发生在世界各稻区最严重的真菌性病害，做好对该病的防治对水稻高产与稳产至关重要。随着生物技术的迅速发展，组织培养的离体筛选技术作为一种获得抗病突变体的高效手段而被广泛应用于水稻抗病育种研究。综述了抗稻瘟病细胞突变体筛选中愈伤组织的诱导、粗毒素的制备及毒素对材料抗性筛选等3个主要技术环节，并对抗病突变体在抗病育种中的应用提出问题及展望。

关键词: 残留, 残留

Abstract:

Blast is the most serious rice diseases in the world. The prevention and controlling of it is therefore significant to the agriculture production. With the rapid development of bio-technology, an in vitro screening technique system in tissue culture for rice (Oryza sativa L. subsp. indica) was widely applied as an effective method to the resistant breeding of rice. We review three main aspects of this technique: the callus induction, the preparation of crude toxin factor and the selection of toxin resistant materials. Furthermore, we introduce questions and prospects of the application of these mutants in rice resistant breeding.