欢迎访问《中国农学通报》,

中国农学通报 ›› 2011, Vol. 27 ›› Issue (26): 65-68.

所属专题: 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

肉种鸡免疫两不同ND-IB-IBD-Reo四联灭活疫苗后的抗体水平比较

徐守振 王 艳   

  • 收稿日期:2011-06-14 修回日期:2011-07-18 出版日期:2011-10-15 发布日期:2011-10-15
  • 基金资助:

    规模化鸡场主要流行疫病的快速分子检测技术集成研究与应用

Comparison of the Immune Antibody after Immunized with Different ND-IB-IBD- Reo Combined inactivated Vaccines in Breeder

  • Received:2011-06-14 Revised:2011-07-18 Online:2011-10-15 Published:2011-10-15

摘要:

为了检测肉种鸡开产前免疫两不同的鸡新城疫-传染性支气管炎-传染性法氏囊病-病毒性关节炎四联灭活疫苗(ND-IB-IBD-Reo四联苗)后的抗体水平差异,在鸡群20周龄分别用A、B疫苗免疫,免疫后28 d和56 d 用HI检测ND抗体水平和ELISA检测IB、IBD和Reo的抗体水平。结果显示,免疫后28 d和56 d,B疫苗的IBD和REO抗体效价效价显著高于A疫苗(P﹤0.05),ND和IB的抗体效价虽较A疫苗高但差异不显著(P﹥0.05)。结果表明,B疫苗的免疫抗体水平优于A疫苗,该研究为肉种鸡ND-IB-IBD-Reo四联灭活疫苗的筛选和合理免疫程序的制定提供依据。

关键词: 烤烟, 烤烟, 氮素, 酸性致香物质, 碱性致香物质

Abstract:

To detect the differences of antibody level in breeders immunized by two different ND-IB-IBD-Reo combined inactivated vaccines before laying, two chicken flocks were inoculated with A and B vaccine respectively at the age of 20 weeks. At 28 d and 56 d after immunization the HI antibody of ND was detected by HI test and antibodies of IB, IBD and Reo were detected by ELISA. The results showed that at 28 d and 56 d after immunization the antibody titers of IBD and Reo in group immunized with B vaccine were significantly higher than those of IBD and Reo in group immunized with A vaccine(P﹤0.05). The antibody titers of ND and IB in group B were higher compared with those of ND and IB in group A, but the difference were not significant(P﹥0.05).The research suggests that B vaccine is better than A vaccine based on antibody levels, and provides the foundation not only for the selection of ND-IB-IBD-Reo combined inactivated vaccine in breeder but also for the development of reasonable immunization programs.