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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (30): 49-54.

所属专题： 生物技术； 棉花

• 农学 农业基础科学 • 上一篇    下一篇

棉花品种基因纯度和特异性的SSR标记分析

刘国栋 钟文 曲辉英 王芙蓉 张军   

  • 收稿日期:2011-06-20 修回日期:2011-09-14 出版日期:2011-11-25 发布日期:2011-11-25

Analysis on Gene Purity and Specificity of Cotton (Gossypium hirsumtum L.) Varieties by SSR Marker

  • Received:2011-06-20 Revised:2011-09-14 Online:2011-11-25 Published:2011-11-25

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摘要：

为从分子水平上鉴定棉花品种的纯度和真实性，采用扩增带型清晰，多态性较高的SSR引物对51个常规棉品种进行了基因纯度鉴定、遗传聚类分析和品种特异性鉴定。基因纯度鉴定结果显示，69%的品种基因纯度较好，基因纯度在90%以上；12%的品种基因纯度较差，基因纯度在80%以下。聚类分析结果表明，所有品种分为2个大类和4个亚类，这反映出品种间的遗传基础较窄。品种特异性鉴定中，以遗传距离阈值T=0.01为划分标准，83%的品种具有特异性。这说明利用SSR标记可以有效鉴定棉花品种的基因纯度和特异性。

关键词: 叶片含水率, 叶片含水率, 光谱反射率, 光谱指数, 柑橘

Abstract:

In order to identify cotton variety and test seed purity at the molecular level, gene purity and variety specificity of 51 cotton (G.hirsumtum L.) varieties were characterized by SSR primer pairs with high polymorphisms and stable amplified bands. The results showed that: most (69%) of the tested varieties with gene purity greater than 90%, while 12% of them with gene purity less than 80%. Genetic cluster analysis based on the marker genotypes indicated that all of the tested varieties were divided into two groups and four sub-groups. And 83% of the tested varieties were distinct one another using a genetic distance threshold of 0.01. All the results suggested that the SSR markers were suitable for and effective on characterization of gene purity and variety specificity of cotton varieties.