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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (30): 32-34.

所属专题： 生物技术； 玉米

• 农学 农业基础科学 • 上一篇    下一篇

不同基因型玉米幼苗对低磷条件的响应

丁艳 韩卓 王泽港 葛才林 戎杰 王敏   

  • 收稿日期:2011-07-04 修回日期:2011-09-12 出版日期:2011-11-25 发布日期:2011-11-25
  • 基金资助:

    江苏省作物栽培生理重点实验室开放课题;蚌埠学院自然科学重点项目

Response of Low Phosphorus Condition on Different Genotypes of Maize

  • Received:2011-07-04 Revised:2011-09-12 Online:2011-11-25 Published:2011-11-25

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摘要：

为探明低磷胁迫对不同磷敏感型玉米的形态及部分理化指标的影响，以不同基因型玉米品种（耐磷型66和磷敏感型163）为材料，采用溶液培养法，对玉米幼苗进行不同处理[加磷(+P)、缺磷(-P)]，研究玉米叶绿素含量、磷酸酯酶含量以及根系的形态指标等的变化。结果表明，在低磷胁迫下(-P)，2个玉米品种的酸性磷酸酶和根系的形态指标均明显大于正常供磷水平下(+P)；叶绿素含量均呈现下降的趋势。另外，低磷条件下，磷敏感型品种163的主根长度远远大于耐磷型品种66的主根长度；而66的叶绿素含量和酸性磷酸酶含量均大于163。这些结果表明，不同基因型玉米品种在低磷条件下的适应性反应，为进一步研究磷饥饿响应基因在植物营养中的功能提供理论参考。

关键词: 马铃薯干腐病, 马铃薯干腐病, 镰刀菌, DNA提取方法, 改良SDS法, 2×, CTAB法

Abstract:

Based on different maize genotypes as material, using solution culture method, on maize seedling of different treatments [phosphor (+P), phosphorus (-P)], study the changes, such as maize chlorophyll content, acid phosphatase activity and root morphology index etc. The results showed that, in low phosphorus stress (-P), acid phosphatase and root morphological indicators of two maize varieties were significantly greater than normal P supply levels (+P), and chlorophyll content showed a downward trend. In addition, under the condition of low phosphorus, taproot length of phosphorus sensitive varieties 163 was much greater than that of the resistance phosphorus varieties 66, while the chlorophyll content and the activity of acid phosphatase of 66 were greater than that of 163. These results suggested that, adaptive responses in different maize genotypes under low phosphorus condition, and provide a theoretical reference for the further study of phosphorus starvation response gene in plant nutrition function.

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