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中国农学通报 ›› 2013, Vol. 29 ›› Issue (6): 199-202.doi: 10.11924/j.issn.1000-6850.2012-2860

• 植物保护 农药 • 上一篇    下一篇

青枯劳尔氏菌潜在新寄主鉴定与青枯病防治策略的思考

李鹏 吴毅歆 毛自朝 何月秋   

  • 收稿日期:2012-08-20 修回日期:2012-10-06 出版日期:2013-02-25 发布日期:2013-02-25
  • 基金资助:
    科技部国际科技合作项目(2009DFA32360)。

Identification of the Potential New Hosts of Ralstonia solanacearum and Thinking of Control Strategies for Bacterial Wilt

  • Received:2012-08-20 Revised:2012-10-06 Online:2013-02-25 Published:2013-02-25

摘要: 为了充分发挥农业措施管理,合理轮套作技术在防治农作物青枯病的作用,结合云南当地农业生态实际情况,采用分离自雪莲果青枯病植株上的青枯劳尔氏菌,在28℃温室条件下,遵循柯赫氏法则,接种、分离、再接种和再分离该病菌,完成了植物病害验证过程。结果表明:在接种后,杜氏鼠尾草、曼陀罗和鸭趾草等3种昆明地区田间常见杂草均出现青枯病典型症状,首先在接种部位出现黑色病斑,继而植株萎蔫,并有大量的乳白色菌脓溢出。结合在自然界下尚未发现这3种杂草感染青枯病和在根部有青枯劳尔氏菌富集的研究结果,这3种杂草被推断为青枯劳尔氏菌的潜在寄主。本研究结果对农作物青枯病防治策略有重要指导意义。

关键词: 降解, 降解

Abstract: In order to prevent crops bacterial wilt by making full use of agricultural management, reasonable rotation and interplanting technology, combined with the actual agricultural ecology of Yunnan Province, three common weeds in the field of Kunming, Salvia dugesii Fernald, Dature stramonium Datura L., and Commelina communis L. were inoculated with Ralstonia solanacearum in the greenhouse at 28℃, by following Koch's postulates: inoculation, isolation, re-inoculation and re-isolation to verify the bacterial pathogenicity. The results showed that after inoculation, the three weeds produced typical symptoms of bacterial wilt, i.e. black lesion appeared firstly at the inoculation site, and then whole plant wilt, and lots of bacterial ooze were observed. With the consideration of the fact that the three weeds were not infected by R. solanacearum in the nature and enrich the bacterium at their roots, it was inferred that they should be the bacterial potential hosts. These results could provide references to make management strategies for crops bacterial wilt.