组织特异性启动子在作物基因工程中的研究进展

doi:10.11924/j.issn.1000-6850.2013-1989

摘要/Abstract

摘要： 作物基因工程中常使用组成型启动子驱动外源基因在转基因作物中高效表达，但组成型启动子不能从时间上和空间上调控外源基因的表达。可以采用组织特异性启动子，在特定器官或组织中表达外源基因，从而减轻组成型启动子对转基因作物的不良影响，使目标基因的表达产物在特定部位积累。本研究通过对组织特异性启动子的特征进行了简要阐述，重点归纳了组织特异性启动子的分类，分析了其在作物基因工程中的最新研究进展，并探讨了其在作物基因工程应用中存在的问题与前景。

关键词: 综合评价, 综合评价

Abstract: In the crop genetic engineering, constitute promoters have been used to make foreign gene express efficiently in transgenic crops, but constitute promoters are unable to regulate gene expression temporally and spatially. Tissue-specific promoters can be employed to alleviate the drawbacks of constituted promoters in transgenic crops. The target genes driven by tissue-specific promoters are expressed in special tissue or organ, and the target proteins are accumulated in special parts. This review was a brief introduction to tissue-specific promoter characters, mainly focusing on the category of tissue-specific promoters and the up-to-date research progress in crop genetic engineering. Furthermore, the limits and prospects of tissue-specific promoters in crop genetic engineering were discussed.

贺红霞陈亮林春晶柳青. 组织特异性启动子在作物基因工程中的研究进展[J]. 中国农学通报, 2014, 30(9): 225-231.

参考文献

[1] Potenza C, Aleman L, Sengupta- Gopalan C. Targeting transgene expression in research, agricultural, and environmental applications: promoters used in plant transformation[J]. In Vitro Cell Dev Biol Plant,2004,40:1-22.
[2] 皮灿辉,易自力,王志成.提高转基因植物外源基因表达效率的途径[J].中国生物工程杂志,2003,23:1-4.
[3] 尹涛,张上隆,刘敬梅,等.果实特异性启动子研究现状及其应用[J].农业生物技术学报,2009,17:355-360.
[4] Edwards J W, Walker E L, Coruzzi G M. Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase[J]. Proceeding of the National Academy of Sciences,1990,87:3459-3463.
[5] 任茂智.棉花 nodulin-like和 arf1基因及其启动子的分离和功能分析[D].北京:中国农业科学院,2004.
[6] Fauteux F, Str?mvik M V. Seed storage protein gene promoters contain conserved DNA motifs in Brassicaceae, Fabaceae and Poaceae[J]. BMC Plant Biology,2009,9:126-136.
[7] Huda K M K, Banu M S A, Pathi K M, et al. Reproductive organ and vascular specific promoter of the rice plasma membrane Ca2+ ATPase mediates environmental stress responses in plants[J]. PLoS ONE,2013,8:E57803(1-15).
[8] 糜赛男,曹明富.植物组织特异性启动子的研究进展[J].生物学教学,2010,35:2-4.
[9] 李宁,樊守金,张增艳.植物抗病相关启动子及其研究进展[J].植物遗传资源学报,2007,8:234-239.
[10] 聂丽娜,夏兰琴,徐兆师,等.植物基因启动子的克隆及其功能研究进展[J].植物遗传资源学报,2008,9:385-391.
[11] 宋扬,周军会,张永强.植物组织特异性启动子研究[J].生物技术通报,2007,6:21-24.
[12] Shi Y, Wang M B, Powell K S, et al. Use of the rice sucrose synthase- 1 promoter to direct phloem- specific expression of β- glucuronidase and snowdrop lectin genes in transgenic tobacco plants[J]. Journal of Experimental Botany,1994,45:623-631.
[13] 刘昱辉,贾世荣.维管束特异表达启动子及其顺式调控元件[J].生物工程学报,2003,19:131-135.
[14] 胡桂兵,张上隆,徐昌杰等.笋瓜韧皮部特异性启动子的克隆分析及新型植物表达载体构建[J].江西农业大学学报,2005,27:4-8.
[15] Gurr S J, Rushton P J. Engineering plants with increased disease resistance: how are we going to express it? Trends in Biotechnology, 2005,23:283-290.
[16] Truernit E, Sauer N. The promoter of the Arabidopsis thaliana SUC2 sucrose- H + symporter gene directs expression of betaglucuronidase to the phloem: evidence for phloem loading and unloading by SUC2[J]. Planta,1995,196:564-570.
[17] 蒋浩,秦红敏,虞红梅,等.笋瓜韧皮部蛋白基因启动子的克隆及其功能初探[J].农业生物技术学报,1999,7:63-68.
[18] Guo H, Chen X, Zhang H, et al. Characterization and activity enhancement of the phloem- specific pumpkin PP2 gene promoter [J]. Transgenic Research, 2004,13:559-566.
[19] 郭洪年. NPP2基因启动子及抗虫转基因植物的研究[D].北京:中国科学院微生物研究所,2002.
[20] 于文静,王玉富,邱财生,等.韧皮部特异性启动子研究概况[J].中国麻业科学,2010,32:116-122.
[21] 张海利,吕淑霞,田颖川.韧皮部特异性启动子研究概述[J].中国生物工程杂志,2003,23(11):11-15..
[22] 夏江东,程在全,吴渝生,等.高等植物启动子功能和结构研究进展[J].云南农业大学学报,2006,21:7-14.
[23] Shirsat A H, Wilford N, Evans I M, et al. Expression of a Brassica napus extensin gene in the vascular system of transgenic tobacco and rape plants[J]. Plant Molecular Biology,1991,17:701-709.
[24] Elliott K A, Shirsat A H. Promoter region of the extA extensin gene from Brassica napus control activation in response to wounding and tensile stress[J]. Plant Molecular Biology,1998,37:675-687.
[25] Saha P, Chakraborti D, Sarkar A, et al. Characterization of vascularspecific RSs1 and rolC promoters for their utilization in engineering plants to develop resistance against hemipteran insect pests[J]. Planta,2007,226:429-442.
[26] 李钊,庄洪涛,杜丽璞,等.组织特异表达启动子 RSS1P在转 TiERF1基因小麦中的应用[J].作物学报,2011,37:1897-1903.
[27] Tiimonen H, H?ggman H, Tsai C J. The seasonal activity and the effect of mechanical bending and wounding on the PtCOMT promoter in Betula pendula Roth [J]. Plant Cell Reports,2007,26: 1205-1214.
[28] 张爽,武会,杜克久,等.几个杨树木质部特异启动子的分离与序列分析[J].蚕业科学,2009,35(2):219-222
[29] 张爽,武会,王志刚,等.杨树木质部特异启动子 MDCesAP 的改造及功能检测[J].蚕业科学,2010,36:304-307.
[30] 张爽,武会,杜克久.几个杨树木质部特异启动子片段表达载体的构建与功能分析[J].蚕业科学,2012,38:0199-0203.
[31] Trindade L M, Horvath B, Bachem C, et al Isolation and functional characterization of a stolon specific promoter from potato (Solanum tuberosum L.) [J]. Gene,2003,303:77-87.
[32] 宋东光,王光清,汪训明. Patatin启动子驱动的乙肝表面抗原基因在马铃薯中的表达[J].高科技通讯,2000,10:18-20.
[33] 陈国梁,陈宗礼,贺晓龙,等.马铃薯块茎组织特异性启动子的克隆及序列分析[J].生物技术通报,2011:69-72.
[34] 陈国梁,陈宗礼,齐向英,等.马铃薯块茎组织特异性启动子 GBSS的克隆及序列分析[J].长江蔬菜,2011:25-27.
[35] Koyama T, Ono T, Shimizu M, et al. Promoter of Arabidopsis thaliana phosphate transporter gene drives root-specific expresssion of transgene in rice[J]. Journal of Bioscience and Bioengineering, 2005,99:38-42.
[36] Gu R, Zhao L, Zhang Y, et al. Isolation of a maize beta-glucosidase gene promoter[J]. Plant Cell Reports,2006,25:1157-1165.
[37] Vaughan S P, James D J, Lindsey K, et al. Characterization of FaRB7, a near root- specific gene from strawberry (Fragaria x ananassa Duch.) and promoter activity analysis in homologous and heterologous hosts[J]. Journal of Experimental Botany,2010,57: 3901-3910.
[38] 李志邈,杨悦俭,杨飞,等.番茄根特异表达基因 LeGRP2启动子的克隆及其在拟南芥的表达分析[J].中国农业科学,2010,43:1877- 1882.
[39] 王淼,王旭静,唐巧玲,等.高等植物绿色组织特异表达启动子的研究进展.中国农业科技导报,2012,12:33-37.
[40] 陈爱敏.水稻茎叶特异性启动子研究进展[J].现代农业科技,2012: 16-17,19.
[41] Cai M, Wei J, Li X, et al. A rice promoter containing both novel positive and negative cis- elements for regulation of green tissuespecific gene expression in transgenic plants[J]. Plant Biotechnol J., 2007,5:664-674.
[42] Thilmony R, Guttman M, Thomson J, et al. The LP2 leucine- rich repeat receptor kinase gene promoter directs organ- specific, lightresponsive expression in transgenic rice[J]. Plant Biotechnol J., 2009,7:867-882.
[43] Ye R, Zhou F, Lin Y. Two novel positive cis- regulatory elements involved in green tissue- specific promoter activity in rice(Oryza sativa L.ssp.) [J]. Plant cell Rep.,2012,231:1159-1172.
[44] Noh Y S, Amasino R M. Identification of a promoter region responsible for the senescence- specific expresssion of SAG12. Plant Molecular Biology[J],1999,41:181-194.
[45] Liu L, Zhou Y, Szczerba M W, et al. Identification and application of a rice senescence-associated promoter[J]. Plant Physiology,2010, 153:1239-1249.
[46] 瞿礼嘉,范战民.玉米萌发类似蛋白及其编码基因与应用[P],中国专利, 02158099.5,2004.07.07
[47] 瞿礼嘉,范战民.玉米萌发类似蛋白基因启动子及其应用[P].中国专利, 02158113.4,2004.07.07
[48] 范战民.玉米幼嫩心叶特异基因 Zmg1p1的克隆、表达分析及启动子初步研究[D].北京:北京大学, 2004.
[49] 徐志超,陆晓茜,杜娟,等. Leafy启动子控制下 5-烯醇式丙酮酸-莽草酸-3-磷酸合酶基因（CP4EPSPS）的表达增强芽对草甘膦的抗性[J].农业生物技术学报,2012,20:23-29.
[50] 姚嵘,马三梅.植物果实的特异型启动子[J].生命的化学,2006,26: 0336-0338.
[51] Park J I, Lee Y K, Chung W I, et al. Modification of sugar composition in strawberry fruit by antisense suppression of an ADPglucose pyrophosphorylase[J]. Molecular Breeding,2006,17:269- 279.
[52] Atkinson R G, Bolitho K M, Wright M A, et al. Apple ACC-oxidase and polygalacturonase: ripening- specific gene expression and promoter analysis in transgenic tomato[J]. Plant Molecular Biology, 1998,38:449-460.
[53] Schaart J G, Salentijn E M J, Krens F A. Tissue-specific expression of the β- glucuronidase reporter gene in transgenic strawberry (Fragaria × ananassa) plants[J]. Plant Cell Reports,2002,21:313-319.
[54] 郭淑华,杨宝军,杜永臣,等.番茄果实特异表达启动子 E8p1的克隆及表达[J].农业生物技术学报,2007,15:362-364.
[55] 尹涛,卢虹玉,张上隆,等.甜蛋白 Brazzein基因在番茄果实中的特异表达[J].遗传,2009,31:663-667.
[56] Kim M J, Kim H, Suh M C. Seed- specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5'- UTR intron [J]. Molecular Genetics and Genomics,2006,276:351-368.
[57] Kim M J, Kim J K, Suh M C. The SebHLH transcription factor mediates trans- activation of the SeFAD2 gene promoter through binding to E- and G- box elements [J]. Plant Molecular Biology, 2007,64:453-466.
[58] Rasmussen T B, Donaldson I A. Investigation of the endospermspecific sucrose synthase promoter from rice using transient expression of reporter genes in auar seed tissue[J]. Plant Cell Reports,2006,25:1035-1042.
[59] Li W, Bai L, Chai Z, et al. Evaluation of seed storage protein gene 3'- untranslated regions in enhancing gene expression in transgenic rice seed[J]. Transgenic Research,2012,21:545-553.
[60] 李吉涛,郭建春.种子特异性启动子的研究进展[J].安徽农业科学, 2008,36:1382-1385.
[61] 张梦晗,谢伟红,曾洪斌,等.种子特异启动子的克隆及植物表达载体构建[J].安徽农业科学,2010,38:63-67.
[62] Choi H W, Lemaux P G, Cho M J. Long-term stability of transgene expression driven by barley endosperm- specific hordein promoters in transgenic barley[J]. Plant Cell Reports,2003,21:1108-1120.
[63] 蒋淑丽,李涛.植物启动子及其相关功能研究概述[J].陕西农业科学,2012,58:94-97.
[64] 郑少缘,范燕萍.花器管特异启动子的研究进展[J].安徽农业科学, 2011,39:19067-19069.
[65] 路静,赵华燕,何奕昆,等.高等植物启动子及其应用研究进展[J].自然科学进展,2004,14:856-862.
[66] Roque E, Gomez M D, Ellul P, et al. The PsEND1 promoter: a novel tool to produce genetically engineered male-sterile plants by early anther ablation[J]. Plant Cell Reports,2007,26:313-325.
[67] Luo H, Lee J Y, Hu Q, et al. RTS, a rice anther- specific gene is required for male fertility and its promoter sequence directs tissuespecific gene expression in different plant species[J]. Plant Molecular Biology,2006,62:397-408.
[68] 汪迎春,孙勇如,张利明,等.植物花药花粉特异性基因的调控序列[J].生物工程进展,2000,20:52-54,13.