现代生物技术在亚麻育种中的应用

doi:10.11924/j.issn.1000-6850.casb15040011

中国农学通报 ›› 2015, Vol. 31 ›› Issue (23): 58-63.doi: 10.11924/j.issn.1000-6850.casb15040011

所属专题：生物技术

现代生物技术在亚麻育种中的应用

冯学金,杨建春

山西省农业科学院高寒区作物研究所,山西省农业科学院高寒区作物研究所

收稿日期:2015-04-01 修回日期:2015-07-10 接受日期:2015-05-22 出版日期:2015-08-20 发布日期:2015-08-20
通讯作者: 冯学金
基金资助:
山西省回国留学人员科研资助项目“优质、高产、抗旱胡麻新品种选育”(2015-117)；农业部黄土高原作物基因资源与种质创制重点实验室项目。

Application of Modern Biotechnology in Flax Breeding

Received:2015-04-01 Revised:2015-07-10 Accepted:2015-05-22 Online:2015-08-20 Published:2015-08-20

摘要/Abstract

摘要： 为了给亚麻种质资源创新提供新的思路，推动亚麻育种研究的进一步发展，简要介绍了亚麻育种研究现状，总结了基因工程技术、植物组织培养技术和分子标记技术在亚麻育种中的应用。并提出加强远缘杂交育种研究、利用分子标记辅助选择提高杂交育种的效率和效果是亚麻育种研究的重点；建立和完善亚麻花药培养技术，是建立高效亚麻育种技术体系、培育突破性新品种的关键；建立高效的转基因育种技术体系是亚麻育种研究的重要方向。

关键词: 苯醚甲环唑, 苯醚甲环唑, 丙环唑, 乳油, 气相色谱（配ECD检测器）, 农药残留

Abstract: In order to provide new ideas for flax germplasm innovation and promote further development of flax breeding, current research status of flax breeding was described briefly, and the application of gene engineering, plant tissue culture and molecular marker in flax breeding was summarized in the paper. We proposed that the emphasis of flax breeding was to strengthen the study on distant hybridization and improve the efficiency and effect of hybridization breeding by using marker- assisted selection. Establishing and perfecting an effective technical system of anther culture were the keys to establish a practical breeding technical system and breed elite new cultivars. Furthermore, establishing an efficient genetic transformation breeding technical system was an important direction of flax breeding.

冯学金,杨建春. 现代生物技术在亚麻育种中的应用[J]. 中国农学通报, 2015, 31(23): 58-63.

参考文献

[1] Basiran N, Armitage P, Scott R J, et al. Genetic transformation of flax(Linum usitatissimum) by agrobacterium tumefaciens: Regeneration of transformed shoots via a callus phase[J]. Plant Cell Reports, 1987, 6:396-399.
[2] Jordan M, McHughen A. Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer[J]. Plant Cell Reports, 1988,7(4) ：281-284.
[3] McHughen A. CDC Triffid transgenic flax[J].Canadian Journal of plant science,1997, 77(4):641-643.
[4] Yemets AI, Bayer OA, Radchuk VV, et al. Agrobacterium-Mediated transformation of flax with a mutant tubulin gene responsible for resistance to dinitroaniline herbicides[J]. Russian Journal Genetics, 2009, 45(10): 1215- 1222.
[5] 李闻娟,张建平,陈芳,等. Bar基因的亚麻花粉管通道法转化[J].中国农学通报,2013,29(12):96-100.
[6] 贾婉琪. 亚麻高效再生体系优化及抗除草剂bar基因的遗传转化研究[D].长沙:中国农业科学院,2011:30-39.
[7] Wróbel-Kwiatkowska M, Lorenc-Kukula K, Starzycki M, et al. Expression of β-1,3-glucanase in flax causes increased resistance to fungi[J]. Physiological and Molecular Plant Pathology, 2004, 65(5): 245-256.
[8] 苑志辉.兔防御素NP-Ⅰ基因在亚麻抗枯萎病方面的研究[D].北京:中国农业大学,2005:5-15.
[9] Tadeusz Czuj, Magdalena Zuk, Michal Starzycki, et al. Engineering increases in sulfur amino acid contents in flax by overexpressing the yeast Met25 gene[J].Plant Science, 2009, 177:584–592.
[10] 王毓美,徐云远,贾敬芬.亚麻遗传转化体系的建立及几丁质酶基因导入的研究[J].西北植物学报,2000,20(3):346-351.
[11] Lorenc-Kuku?a K,Wróbel-Kwiatkowska M,Starzycki M,et al. Engineering flax with increased flavonoid content and thus Fusarium resistance[J]. Physiological and Molecular Plant Pathology, 2007 , (70): 38–48.
[12] Mierziak J, Wojtasik W, Kostyn K, et al. Crossbreeding of transgenic flax plants overproducing flavonoids and glucosyltransferase results in progeny with improved antifungal and antioxidative properties[J]. Molecular Breeding, 2014, 34(4):1917–1932.
[13] 王秀珍. 农杆菌介导的月见草△6-脂肪酸脱氢酶基因转化亚麻的研究[D].呼和浩特:内蒙古大学,2010:45-49.
[14] Abbadi A, Domergue F, Bauer J, et al. Biosynthesis of very-long chain polyunsaturated fatty acids in transgenic oilseeds ：constraints on their accumulation[J]. Plant Cell, 2004, 16(10) ：2734-2748.
[15] 苑志辉,孙洪涛.亚麻体细胞无性系的建立及其植株再生[J].中国麻作,1997,(1):17一18.
[16] A. McHughen, M. Swartz. A Tissue-Culture Derived Salt-Tolerant Line of Flax (Linum usitatissimum)[J]. Journal of Plant Physiology, 1984, 117(2): 109–117.
[17] Mark C. Jordan,SAlan McHughen. Selection for Chlorsulfuron Resistance in Flax (Linum usitatissimum) Cell Cultures[J]. Journal of Plant Physiology, 1987,131(3–4): 333–338.
[18] Nichterlein K, Umbach H, Friedt W. Genotypic and exogenous factors affecting shoot regeneration from anther callus of linseed (Linum usitatissimum L.)[J]. Euphytica, 1991, 58(2): 157-164.
[19] I. Rutkowska-Krause,G. Mankowska,M. Lukaszewicz et al. Regeneration of flax (Linum usitatissimum L.) plants from anther culture and somatic tissue with increased resistance to Fusarium oxysporum[J]. Plant Cell Rep,2003, (22):110-116.
[20] W. Friedt,C. Bickert and H. Schaub. In vitro breeding of high-linolenic, doubled-haploid lines of linseed (Linum usitatissimum L.) via androgenesis[J]. Plant Breeding, 1995, 114(4):322- 326.
[21] 宋淑敏,田玉杰,姬妍茹,等.γ射线辐射亚麻花药的研究初报[J].中国麻业,2004,26(4)：162-163.
[22] 康庆华,许修宏,李柱钢,等. 亚麻单倍体抗逆基因的转化[J].中国麻业,2006,28(6)：291-296.
[23] T.Y. Bo, J.J.Ma, J.X. Chen, et al. Identification of specific molecular markers linked to the rust resistance gene M4 in flax[J]. Australasian Plant Pathology, 2008, 37: 417-420.
[24] 薄天岳,叶华智,李晓兵,等.亚麻抗枯萎病基因FuJ7(t)的分子标记[J].中国农业科学,2003,36(3):287-291.
[25] G. Hausner, K.Y. Rashid, E.O. Kenaschuk, et al. The identification of a cleaved amplified polymorphic sequence (CAPS) marker for the flax rust resistance gene M3 [J]. Canadian Journal of Plant Pathology, 1999, 21: 187-192 .
[26] G. Hausner, K.Y. Rashid, E.O. Kenaschuk, et al. The development of co-dominant PCR/RFLP based markers for the flax rust resistance alleles at the L locus [J]. Genome, 1999, 42: l-8.
[27] T.J.Oh, M.Gorman, C.A.Cullis. RFLP and RAPD mapping in flax (Linum usitatissimum) [J]. Theor Appl Genet, 2000,(101):590–593.
[28] W. Spielmeyer, A.G.Green, D. Bittisnich . Identification of quantitative trait loci contributing to Fusarium wilt resistance on an AFLP linkage map of flax (Linum usitatissimum) [J]. Theor Appl Genet (1998) 97: 633-641.
[29] Sylvie Cloutier,Raja Ragupathy,Zhixia Niu, et al. SSR-based linkage map of flax (Linum usitatissimum L.) and mapping of QTLs underlying fatty acid composition traits[J]. Mol Breeding, 2011, (28):437–451.

现代生物技术在亚麻育种中的应用

Application of Modern Biotechnology in Flax Breeding

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	强生军, 刘玉荣, 李刚. 溶剂标、基质标对农药残留检测结果的影响及校正[J]. 中国农学通报, 2022, 38(4): 99-106.
[2]	卢珍萍, 田英. 中国蔬果中农药残留的现状及其去除方法[J]. 中国农学通报, 2022, 38(24): 131-137.
[3]	陈锋, 孟顺龙, 陈家长. 农药在沉积物-水-生物体的污染特征综述[J]. 中国农学通报, 2021, 37(7): 159-164.
[4]	王东鹏, 叶诚, 廖小丽. 微流控芯片在农产品安全检测中的应用[J]. 中国农学通报, 2021, 37(36): 148-154.
[5]	刘琳, 马腾飞, 贾孙悦, 周芹, 王皙玮. 改良QuEChERS方法结合气相色谱测定红甜菜中20种农药残留[J]. 中国农学通报, 2021, 37(35): 110-117.
[6]	田耿智. 基地蔬菜水果中农药残留暴露风险和预警风险评估[J]. 中国农学通报, 2021, 37(27): 112-116.
[7]	李琦, 郭文磊, 孟琦, 于金萍, 刘亦学, 张惟. 27%二甲戊灵·砜吡草唑乳油防治棉花田一年生杂草效果与安全性[J]. 中国农学通报, 2021, 37(25): 121-124.
[8]	郭思依, 孙明娜, 董旭, 褚玥, 童舟, 王梅, 高同春, 段劲生. 免疫分析技术在农药残留快速检测中的应用及研究进展[J]. 中国农学通报, 2021, 37(23): 106-112.
[9]	王岩, 彭强, 赵小明, 尹恒. 生物降解农药残留的研究进展[J]. 中国农学通报, 2021, 37(18): 117-124.
[10]	于婷婷, 邱鹏程, 陈强, 石富, 杨雅钧. 气相色谱法测定蔬菜和水果中有机磷及拟除虫菊酯类农药残留不确定度分析[J]. 中国农学通报, 2021, 37(17): 129-136.
[11]	纪力, 邵文奇, 陈富平, 董青君, 章安康. 连年规模稻鸭共养对稻田土壤性状、稻米产量及品质的影响[J]. 中国农学通报, 2021, 37(13): 1-7.
[12]	溥丽华, 郎小琴, 穆志国, 赵云, 吴常敏, 吴敏, 张建利. 贵阳城市周边蔬菜规模化种植农药残留风险分析——以贵阳市某区为例[J]. 中国农学通报, 2020, 36(9): 145-149.
[13]	丁怡, 李荣玉, 刘世江, 赵琪君. 高效液相色谱法测定茶叶中的5种农药残留[J]. 中国农学通报, 2020, 36(29): 132-138.
[14]	卜华虎,任志强,肖建红,王晓清,杨慧珍. 玉米抗虫研究进展[J]. 中国农学通报, 2019, 35(30): 145-149.
[15]	李嘉慧. 基于文献计量学的国内外茶叶农药残留研究综述[J]. 中国农学通报, 2019, 35(10): 148-157.