欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2003, Vol. 19 ›› Issue (5): 43-43.

所属专题： 园艺

• 目次 • 上一篇    下一篇

核桃组培中抑制褐化现象初探

张卫芳 ,高疆生 , 欧勇慧 ,扬鹏卫   

  • 出版日期:2003-10-25 发布日期:2003-10-25

Primary Study on Browning Control in Tissue Culture of Pellicular Walnut

Zhang Weifang ; Gao Jiangsheng ; Ou Yonghui ; Yang Pengwei   

  • Online:2003-10-25 Published:2003-10-25

PDF (PC)

826

摘要： 以薄皮核桃茎尖为试材，对其分化生长过程中的褐化现象进行初步研究，经多次试验表明，在抑制褐化及保证茎尖正常分化生长时期，主要采取二步法：①接种于1/2MS+活性碳2g培养基中，置于5℃冰箱中黑暗培养7d后取出；②转接于DKW+6-BA1.0mg/L+IAA0.01mg/L+20%硫代硫酸钠5ml/L培养基中置于25℃，无光照环境下培养，每隔15-20d转换培养基，此方法抑制褐化效果为佳，可确保核桃茎尖正常分化生长的整个阶段

关键词: 金银花, 金银花, 戊唑醇, 苯醚甲环唑, 农药残留

Abstract: Experiment was aimed on studing the problem of browning control during the shoot tip culture of Pellicular Walnut . Results showed that the best way of preventive methods for the browning of explanted tissue is that the explants of Walnut was cultured in the dark on DKW medium added with 6-BA 1.0 mg?L-1 +IAA 0.01 mg?L-1 +20% Na2S2O3 5 ml?L-1 at 25 oC for 15~20d after dark culture on 1/2MS medium added with activated carbon 2g at 5 oC for 7d , and then , transplant the subculture plantlet on the same medium at intervals of 15~20d .