薄皮甜瓜果皮花斑性状的InDel-spot分子标记开发和应用

doi:10.11924/j.issn.1000-6850.casb20191200970

摘要/Abstract

摘要：

为了提高薄皮甜瓜的果皮花斑和非果皮花斑性状育种材料的选择效率,笔者采用田间提供的薄皮甜瓜的果皮花斑材料和非果皮花斑材料进行了研究,于2号染色体上控制甜瓜果皮花斑的基因附近重新开发连锁的InDel-spot分子标记,利用InDel-spot标记对20份薄皮甜瓜材料进行基因检测。结果表明,果皮花斑条带大小约211 bp,而果皮非花斑条带大小约219 bp,且符合率达到95%;进一步利用InDel-spot标记对2个F₂群体及5个F₃群体进行果皮花斑的鉴定,其中Qb978(F₂)、Qb931(F₃)和Qb932(F₃)的检测准确率分别96.6%、91.7%和83.3%。该InDel-spot分子标记在甜瓜果皮花斑的实际鉴定中具有重要的实践运用,可以针对性地提高育种材料选择的效率,缩短育种周期。

关键词: 甜瓜, 果皮花斑性状, 非果皮花斑性状, InDel-spot分子标记, 育种周期

Abstract:

The purpose is to improve the selecting efficiency of breeding materials with the spotted and the non-spotted trait in melons. The authors conducted the research with the spotted and non-spotted melons provided in field, and developed the linked InDel-spot molecular marker near the gene controlling the melon spotted trait in chromosome 2. Twenty melons were genotyped with the InDel-spot maker. The results showed that the size of spotted band was about 211 bp, while the non-spotted band was about 219 bp, with 95% of compliance rate. The InDel-spot marker was further used to identify the spotted and non-spotted trait of 2 (F₂) and 5 (F₃) populations. Among them, the detection accuracy of Qb978 (F₂), Qb931 (F₃) and Qb932 (F₃) was 96.6%, 91.7% and 83.3%, respectively. The results indicate that InDel-spot marker has a great application value in the identification of the spotted and non-spotted trait, which could raise the selecting efficiency of breeding materials and shorten the breeding cycle.

Key words: melon, spotted trait, non-spotted trait, InDel-spot molecular marker, breeding cycle

中图分类号:

S333.6

谢梅婷, 刘莉, 华德平. 薄皮甜瓜果皮花斑性状的InDel-spot分子标记开发和应用[J]. 中国农学通报, 2020, 36(35): 37-41.

Xie Meiting, Liu Li, Hua Deping. An InDel-spot Molecular Marker for the Spotted Trait in Melon: Development and Application[J]. Chinese Agricultural Science Bulletin, 2020, 36(35): 37-41.

图/表 7

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编号	田间编号	果皮类型	变种类型
1	Qa159	花皮	var.chinensis
2	Qa346	花皮	var.chinensis
3	Qa155	花皮	var.chinensis
4	Qa168	花皮	var.chinensis
5	Qa184	花皮	var.chinensis
6	Qa204	花皮	var.chinensis
7	Qa213	花皮	var.chinensis
8	Qa300	花皮	var.chinensis
9	Qa307	花皮	var.chinensis
10	Qa58	黄绿皮	var.chinensis
11	Qa347	黄绿皮	var.chinensis
12	Qa1	白皮	var.chinensis
13	Qa52	黄皮	var.chinensis
14	Qa86	深绿皮	var.chinensis
15	Qa96	绿皮	var.chinensis
16	Qa 420	灰绿皮	var.chinensis
17	Qa384	墨绿皮	var.chinensis
18	Qa392	黄绿皮	var.chinensis
19	Qa 332	灰黄绿皮	var.chinensis
20	Qa12	白皮	var.chinensis

编号	田间编号	果皮类型	变种类型
1	Qa159	花皮	var.chinensis
2	Qa346	花皮	var.chinensis
3	Qa155	花皮	var.chinensis
4	Qa168	花皮	var.chinensis
5	Qa184	花皮	var.chinensis
6	Qa204	花皮	var.chinensis
7	Qa213	花皮	var.chinensis
8	Qa300	花皮	var.chinensis
9	Qa307	花皮	var.chinensis
10	Qa58	黄绿皮	var.chinensis
11	Qa347	黄绿皮	var.chinensis
12	Qa1	白皮	var.chinensis
13	Qa52	黄皮	var.chinensis
14	Qa86	深绿皮	var.chinensis
15	Qa96	绿皮	var.chinensis
16	Qa 420	灰绿皮	var.chinensis
17	Qa384	墨绿皮	var.chinensis
18	Qa392	黄绿皮	var.chinensis
19	Qa 332	灰黄绿皮	var.chinensis
20	Qa12	白皮	var.chinensis