欢迎访问《中国农学通报》,

中国农学通报 ›› 2022, Vol. 38 ›› Issue (19): 30-36.doi: 10.11924/j.issn.1000-6850.casb2021-0781

所属专题: 生物技术 园艺 农业气象

• 林学·园艺·园林 • 上一篇    下一篇

褪黑素引发对干旱胁迫下黄瓜幼苗生理特性的影响

银珊珊1,2(), 周国彦1,2, 顾博文1,2, 武春成1,2, 闫立英1,2, 谢洋1,2()   

  1. 1河北科技师范学院园艺科学技术学院,河北秦皇岛 066004
    2河北省特色园艺种质挖掘与创新利用重点实验室,河北秦皇岛 066004
  • 收稿日期:2021-08-13 修回日期:2022-01-06 出版日期:2022-07-05 发布日期:2022-07-13
  • 通讯作者: 谢洋
  • 作者简介:银珊珊,女,1998年出生,山东德州人,硕士研究生,主要从事蔬菜栽培生理及分子生物学研究。通信地址:066004 河北省秦皇岛市河北大街西段360号,E-mail: yinshsh@163.com
  • 基金资助:
    河北省高等学校科学技术研究项目“设施高端黄瓜短季节生态型全营养栽培基质研发与应用”(ZD2020337);河北省重点研发计划项目(项目“利用菇渣开发新型蔬菜育苗基质及配套育苗技术研究与应用”)(19226922D);河北省农业农村厅项目“河北省现代农业产业技术体系蔬菜创新团队”(HBCT2018030209);河北省教育厅平台项目“河北省高校园艺作物育种应用技术研发中心”(YF201404)

Effects of Melatonin Priming on Physiological Characteristics of Cucumber Seedlings Under Drought Stress

YIN Shanshan1,2(), ZHOU Guoyan1,2, GU Bowen1,2, WU Chuncheng1,2, YAN Liying1,2, XIE Yang1,2()   

  1. 1College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066004
    2Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066004
  • Received:2021-08-13 Revised:2022-01-06 Online:2022-07-05 Published:2022-07-13
  • Contact: XIE Yang

摘要:

研究褪黑素(MT)种子引发对干旱胁迫下黄瓜幼苗生长的影响,以期为MT作为黄瓜种子引发剂或包衣剂物料筛选提供理论依据。以‘津研4号’为试验材料,用50、100、150 μmol/L MT对种子进行6、12、15 h引发处理,以未引发种子为对照,设置50%田间持水量的干旱胁迫,采用基质盆栽,探究MT引发对干旱胁迫下黄瓜幼苗生长的影响。结果表明,50~100 μmol/L的MT引发15 h时对黄瓜幼苗耐旱性增强有显著作用,其中50 μmol/L时提高幼苗的光合速率达85.5%,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性增幅分别为5.1%、42.4%、38.8%,根系活力提高154.0%,说明适宜浓度的MT引发可以通过增强幼苗的抗氧化酶活性及根系活力来提高黄瓜幼苗的耐旱性。

关键词: 黄瓜, 种子引发, 褪黑素, 干旱胁迫, 抗氧化酶活性

Abstract:

The purpose is to study the effect of melatonin (MT) seed priming on cucumber seedling growth under drought stress, so as to provide a theoretical basis for selecting MT as cucumber seed priming agent or coating agent. The seeds were treated with 50, 100, 150 μmol/L melatonin (MT) priming for 6, 12 and 15 h, and the unprimed seeds were used as a control, the drought stress was set at 50% of field moisture capacity, and the substrate pot cultivation was used. The results showed that 50-100 μmol/L of MT priming for 15 h triggered a significant increase in drought tolerance of cucumber seedlings. Compared with the control, 50 μmol/L MT priming increased the photosynthetic rate of the seedlings by 85.5%, and SOD, POD and CAT activities by 5.1%, 42.4% and 38.8%, respectively, and the root vigor was improved by 154.0%, indicating that suitable concentration of MT priming could improve the drought tolerance of cucumber seedlings by enhancing the antioxidant enzyme activities and root vigor of the seedlings.

Key words: cucumber, seed priming, melatonin, drought stress, autioxidant enzyme activity

中图分类号: