柑橘CsaccA基因功能鉴定

doi:10.11924/j.issn.1000-6850.casb2021-0994

中国农学通报 ›› 2022, Vol. 38 ›› Issue (28): 28-35.doi: 10.11924/j.issn.1000-6850.casb2021-0994

所属专题：生物技术；园艺

柑橘CsaccA基因功能鉴定

王超¹^,²(), 杨长耀¹^,³, 李菲菲⁴, 马小川¹^,³, 陈岳文¹^,³, 肖志伟⁵, 尹韬¹^,³, 叶丽¹^,³, 李云松¹^,³, 卢晓鹏¹^,³()

¹湖南农业大学园艺学院,长沙 410128
²中国农业大学园艺学院,北京 100193
³国家柑橘改良中心长沙分中心,长沙 410128
⁴湖南省园艺研究所,长沙 410128
⁵湘西州柑桔科学研究所,湖南吉首 416000

收稿日期:2021-10-20 修回日期:2021-12-17 出版日期:2022-10-05 发布日期:2022-09-28
通讯作者: 卢晓鹏
作者简介:王超,男,1999年出生,山西长治人,硕士在读,研究方向为果树品质调控和逆境生理。通信地址：410128 湖南省长沙市芙蓉区湖南农业大学园艺学院,E-mail： 3564037343@qq.com。
基金资助:
湖南省大学生创新创业训练计划项目“探究CsCAC3基因对柑橘果实品质的影响”(S201910537004);国家自然科学基金“CsbHLH113转录因子调控柑橘果实柠檬酸代谢机制解析”(31872044);现代农业产业技术体系建设专项(CARS-26)

Functional Identification of Citrus CsaccA Gene

WANG Chao¹^,²(), YANG Changyao¹^,³, LI Feifei⁴, MA Xiaochuan¹^,³, CHEN Yuewen¹^,³, XIAO Zhiwei⁵, YIN Tao¹^,³, YE Li¹^,³, LI Yunsong¹^,³, LU Xiaopeng¹^,³()

¹College of Horticulture, Hunan Agricultural University, Changsha 410128
²College of Horticulture, China Agricultural University, Beijing 100193
³National Center for Citrus Improvement (Changsha), Changsha 410128
⁴Hunan Institute of Horticulture, Changsha 410128
⁵Xiangxi Autonomous Prefecture Citrus Science Institute, Jishou, Hunan 416000

Received:2021-10-20 Revised:2021-12-17 Online:2022-10-05 Published:2022-09-28
Contact: LU Xiaopeng

摘要/Abstract

摘要：

为改良柑橘果实品质,以一个推测与柑橘果实品质相关的CsaccA基因为研究对象进行功能鉴定。通过该基因过表达转化Micro-Tom番茄,利用PCR和GUS染色鉴定,植株表型观察和生理生化指标测定等方法系统研究CsaccA基因功能。CsaccA基因显著促进植株的生物量,转基因植株根茎叶生物量均显著高于对照,植株生长速度显著快于对照。转基因植株果实提前转色,果实成熟期明显提前。转基因果实品质分析显示,番茄果实主要的糖组分葡萄糖和果糖含量显著增高,主要的酸组分柠檬酸含量显著降低。CsaccA基因有提升果实糖酸比的功能,可为果实品质改良提供参考。

关键词: 柑橘, 乙酰辅酶A羧化酶, CsaccA基因, 番茄, 转基因, 糖, 酸

Abstract:

This study aims to improve the quality of citrus fruits. Function of a CsaccA gene, which is suspected to be related to citrus fruit quality, was systematically investigated through overexpression in Micro-Tom tomato, identification by PCR and GUS staining, plant phenotype observation and physiological and biochemical determinations. The results showed that the CsaccA gene significantly promoted plant biomass, and the biomass of the root, stem and leaf of transgenic plants were all significantly higher than that of control. The plant growth rate of transgenic plants was significantly faster than that of control as well. The transgenic fruit colored and ripened earlier than that of control. Quality analysis showed that the main sugar components of tomato fruit, glucose and fructose, had a significant increase in content. However, the main acid component citrate had a decrease in content significantly. In summary, transgenic studies have verified that the CsaccA gene functions in increasing fruit sugar-to-acid ratio, which can lay a foundation for fruit quality improvement in the future.

Key words: citrus, acetyl-CoA carboxylase, CsaccA gene, tomato, transgene, sugar, acid

中图分类号:

S666.01

王超, 杨长耀, 李菲菲, 马小川, 陈岳文, 肖志伟, 尹韬, 叶丽, 李云松, 卢晓鹏. 柑橘CsaccA基因功能鉴定[J]. 中国农学通报, 2022, 38(28): 28-35.

WANG Chao, YANG Changyao, LI Feifei, MA Xiaochuan, CHEN Yuewen, XIAO Zhiwei, YIN Tao, YE Li, LI Yunsong, LU Xiaopeng. Functional Identification of Citrus CsaccA Gene[J]. Chinese Agricultural Science Bulletin, 2022, 38(28): 28-35.

图/表 5

图1. 转基因植株阳性鉴定

材料	根		茎		叶
材料	鲜重	干重	鲜重	干重	鲜重	干重
野生型	0.65±0.32	0.11±0.02	3.60±0.75	0.68±0.24	7.37±1.80	0.73±0.14
转基因	6.87±2.05^*	0.44±0.27^*	17.28±4.03^*	4.05±1.13^*	24.01±8.55^*	2.65±1.13^*

表1. 转基因与野生番茄不同组织干重和鲜重 g

图2. 野生型与转基因植株比较

图3. 转基因和野生型番茄发育过程

图4. 转基因与野生番茄果实糖酸含量分析

参考文献 25

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柑橘CsaccA基因功能鉴定

Functional Identification of Citrus CsaccA Gene

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