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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (6): 105-110.doi: 10.11924/j.issn.1000-6850.casb2022-0170

所属专题： 生物技术； 植物保护

• 植物保护·农药 • 上一篇    下一篇

解淀粉芽孢杆菌菌株P-r21抑菌广谱性及对番茄灰霉病的防治

李永丽1(), 源春彦2, 周洲1(), 王春生1, 雷振山1   

  1. 1 信阳农林学院农学院，河南信阳 464000
    2 息县农业科学研究所，河南信阳 464399
  • 收稿日期:2022-03-30 修回日期:2022-06-15 出版日期:2023-02-25 发布日期:2023-02-22
  • 通讯作者: 周洲，男，1978年出生，河南信阳人，教授，博士，研究方向：生物防治。通信地址：464000 河南信阳羊山新区北环路1号信阳农林学院，Tel：13849943528，E-mail：zhouzhouhaust@163.com。
  • 作者简介:

    李永丽，女，1978年出生，河南周口人，高级实验师，博士，研究方向：病害生物防治。通信地址：464000 河南信阳羊山新区北环路1号信阳农林学院，Tel：15896527376，E-mail：。

  • 基金资助:
    信阳市科技攻关项目“作物促生防病微生物菌肥的研制与应用”; 信阳农林学院高水平科研孵化器建设基金项目“链霉菌A-m1杀虫活性的物质基础解析”(FCL202109); 信阳农林学院作物绿色防控与品质调控科技创新团队建设项目(XNKJTD-007)

Bacillus amyloliquefaciens P-r21: Broad-spectrum Antibacterial Effect and Control of Tomato Gray Mold

LI Yongli1(), YUAN Chunyan2, ZHOU Zhou1(), WANG Chunsheng1, LEI Zhenshan1   

  1. 1 College of Agriculture, Xinyang Agriculture and Forestry University, Xinyang, Henan 464000
    2 Agricultural Science Research Institute, Xixian, Xinyang, Henan 464399
  • Received:2022-03-30 Revised:2022-06-15 Online:2023-02-25 Published:2023-02-22

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摘要：

为明确内生解淀粉芽孢杆菌(Bacillus amyloliquefaciens)菌株P-r21的抑菌广谱性及对番茄灰霉病的防治效果，本研究优化了菌株P-r21发酵条件，利用平板对峙法测定了该菌株对6种病原菌的抑菌活性及对番茄灰霉病菌(Botrytis cinerea)孢子萌发的抑制作用，并通过离体接种试验研究了对番茄灰霉病的防效。结果表明：菌株P-r21最佳发酵条件为在PYJ中培养初始pH 6.0，培养时间为12 h。菌株P-r21对供试的6种植物病原菌(Botrytis cinerea、Fusarium proliferatum、Rhizoctonia cerealis、Gaeumannomyces graminis、Fusarium graminearum、Colletotrichum karstii)的生长均表现出抑制作用；对C. karstii和B. cinerea抑制作用最强，抑菌率达到88.0%和84.5%。发酵滤液对B. cinerea孢子萌发抑制率达80.6%，对叶片和果实灰霉病的防效达到100%和80.0%；发酵液10倍稀释后防效也可达到67.1%和60.7%。本研究明确了菌株P-r21具有抑菌广谱性，并可用于番茄灰霉病的防治，为该菌株在植物病害生物防治方面的应用奠定了基础。

关键词: 解淀粉芽孢杆菌, 灰葡萄孢, 生物防治, 发酵条件优化, 抑菌广谱作用

Abstract:

This experiment aims at clarifying the broad-spectrum antibacterial effect of endophytic Bacillus amyloliquefaciens strain P-r21, and determining its control effect on tomato gray mold. In this study, the fermentation conditions of strain P-r21 were optimized. The antibacterial activity of strain P-r21 against six pathogens and its inhibition on spore germination of Botrytis cinerea were determined by plate confrontation method, and its control effect on tomato gray mold was studied by in vitro inoculation test. The results showed that: the optimum fermentation media was PYJ, and the optimum condition was initial pH 6.0 and fermentation time of 12 h. Strain P-r21 exhibited antagonistic ability to six plant pathogens (Botrytis cinerea, Fusarium proliferatum, Rhizoctonia cerealis, Gaeumannomyces graminis, Fusarium graminearum, and Colletotrichum karstii). The inhibition effects of the strain on the growth of C. karstii and B. cinerea were the strongest, and the inhibition rates were 88.0% and 84.5%, respectively. The inhibition rate of B. cinerea spore germination treated with fermentation filtrate was 80.6%. The control effects on tomato gray mold disease of leaves and fruits treated with fermentation filtrate were 100% and 80.0%, respectively. The control effect of fermentation filtrate diluted 10 times could reach 67.1% and 60.7%, respectively. This study clarifies that strain P-r21 has broad-spectrum antibacterial effect and can be used for the control of tomato gray mold. The study lays a foundation for the application of this strain in the biological control of plant diseases.

Key words: Bacillus amyloliquefaciens, Botrytis cinerea, biological control, optimization of fermentation conditions, broad-spectrum antibacterial effect