根内根孢囊霉对正茬和迎茬大豆生物量的影响

doi:10.11924/j.issn.1000-6850.casb2023-0868

中国农学通报 ›› 2024, Vol. 40 ›› Issue (20): 37-45.doi: 10.11924/j.issn.1000-6850.casb2023-0868

根内根孢囊霉对正茬和迎茬大豆生物量的影响

张敏(), 接伟光(), 谭义雯, 阚连宝, 孟剑侠, 孙珊珊, 张勇, 石贺, 张思怡

黑龙江大学，生命科学学院，农业微生物技术教育部工程研究中心，黑龙江省寒区植物基因与生物发酵重点实验室，黑龙江省普通高校微生物重点实验室，哈尔滨 150080

收稿日期:2023-12-22 修回日期:2024-04-15 出版日期:2024-07-11 发布日期:2024-07-11
通讯作者:
接伟光，男，1981年出生，黑龙江哈尔滨人，副教授，博士，主要从事微生物资源挖掘与利用。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学生命科学学院，Tel：0451-86653623，E-mail：jieweiguang2007@126.com。
作者简介:
张敏，女，1999年出生，黑龙江鸡西人，硕士研究生，研究方向：微生物资源挖掘与利用。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学生命科学学院，Tel：0451-86653623，E-mail：1730210122@qq.com。
基金资助:
黑龙江省自然科学基金联合引导项目“根内根孢囊霉与大豆及其根际促生菌的互作机制研究”(LH2023C087); 黑龙江省生态环境保护科研项目“大豆根际促生菌的筛选及其降解土壤农药残留研究”(HST2022TR001); 黑龙江省省属高等学校基本科研业务费科研项目“丛枝菌根真菌与大豆根际促生微生物的互作机制”(2022-KYYWF-1119); 哈尔滨市科技计划自筹经费项目“AM真菌与大豆根际促生菌对大豆抗病性基因表达的影响”(ZC2022ZJ002008)

Effects of Rhizophagus intraradices on Biomass of Rotational Cropping and Alternate Cropping Soybean

ZHANG Min(), JIE Weiguang(), TAN Yiwen, KAN Lianbao, MENG Jianxia, SUN Shanshan, ZHANG Yong, SHI He, ZHANG Siyi

Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education / Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region / Key Laboratory of Microbiology, College of Heilongjiang Province /School of Life Sciences, Heilongjiang University, Harbin 150080

Received:2023-12-22 Revised:2024-04-15 Published:2024-07-11 Online:2024-07-11

摘要/Abstract

摘要：

本研究旨在探究根内根孢囊霉(Rhizophagus intraradices)在缓解大豆迎茬障碍和提高大豆生物量方面的效应。以‘黑农48’品种的大豆为对象，通过湿筛倾析-蔗糖离心法、碱解离-酸性品红染色法以及直接测量法，分别对AM真菌孢子密度、侵染率及大豆生物量进行了测定。结果显示，正茬大豆田的根际土壤中孢子密度是迎茬的2.2倍。在自然土壤条件下，接种R. intraradices的正茬与迎茬土壤中的大豆植株，其根系AM真菌侵染率和生物量均显著高于未接种处理组，其中根长最为显著，分别提高了31.60%和33.57%。在迎茬土壤中接种R. intraradices菌剂的大豆生物量与正茬土壤中未接种处理组的接近。在相同接菌处理条件下，灭菌土壤中的大豆植株根系AM真菌侵染率和生物量均低于自然土壤。本研究这些发现为缓解大豆迎茬障碍和提升大豆产量提供了重要的理论依据。

关键词: 大豆, 正茬与迎茬, 迎茬障碍, 根内根孢囊霉, 侵染率, 生物量, AM真菌孢子密度

Abstract:

The purpose of this study was to investigate the role of Rhizophagus intraradices in alleviating alternate cropping resistance and increasing soybean biomass. The soybean variety ‘Heinong 48’ was used as experimental material. The spore density of arbuscular mycorrhizal (AM) fungi, the colonization rate of AM fungi, and the soybean biomass were determined using wet sieve decantation-sucrose centrifugation, alkali separation-acid fuchsin method and direct measurement, respectively. The results showed that the AM fungi spore density in the rhizosphere soil of rotational cropping soybean field was 2.2 times higher than that of alternate cropping soybean field. Under natural soil conditions, the AM fungal colonization rate of soybean roots and soybean biomass significantly increased after inoculation with R. intraradices in the rotational cropping and alternate cropping soil compared to the untreated group. Additionally, the root length was the most significantly increased by 31.60% and 33.57%, respectively. The soybean biomass inoculated with R. intraradices in the rotational cropping soil was similar to that of the untreated group in the alternate cropping soil. Under the same inoculation conditions, the AM fungal colonization rate of soybean roots and the soybean biomass in the sterilized soil were lower than those in the natural soil. This study laid a theoretical foundation for alleviating the obstacle of soybean alternate cropping resistance and improving soybean yield.

Key words: soybean, rotational cropping and alternate cropping, alternate cropping resistance, rhizophagus intraradices, colonization rate, biomass, arbuscular mycorrhizal fungal spore density

张敏, 接伟光, 谭义雯, 阚连宝, 孟剑侠, 孙珊珊, 张勇, 石贺, 张思怡. 根内根孢囊霉对正茬和迎茬大豆生物量的影响[J]. 中国农学通报, 2024, 40(20): 37-45.

ZHANG Min, JIE Weiguang, TAN Yiwen, KAN Lianbao, MENG Jianxia, SUN Shanshan, ZHANG Yong, SHI He, ZHANG Siyi. Effects of Rhizophagus intraradices on Biomass of Rotational Cropping and Alternate Cropping Soybean[J]. Chinese Agricultural Science Bulletin, 2024, 40(20): 37-45.

图/表 10

参考文献 34

[1]	林凤岩, 黄永娜, 褚洪俊, 等. 我国大豆蛋白加工产业现状及发展趋势[J]. 中国油脂, 2023, 48(11):33-37,56.
[2]	李嘉伦, 李洋洋, 姜凯岩. 氨基寡糖素用于大豆田杀菌剂、叶面肥减量[J]. 现代化农业, 2023(11):5-8.
[3]	何微, 聂迎利, 王晓梅, 等. 全球大豆种子市场现状及中国企业发展启示[J]. 农业展望, 2023, 19(9):40-45.
[4]	COLEMAN K, WHITMORE A P, HASSALL K L, et al. The potential for soybean to diversify the production of plant-based protein in the UK[J]. Science of the total environment, 2021, 767(3):144903.
[5]	JIE W G, LIN J X, GUO N, et al. Community composition of rhizosphere fungi as affected by Funneliformis mosseae in soybean continuous cropping soil during seedling period[J]. Chilean joural of agricultural research, 2019, 79(3):356-365.
[6]	ZHANG W W, FENG Z Z, WANG X K, et al. Quantification of ozone exposure and stomatal uptake-yield response relationships for soybean in Northeast China[J]. Science of the total environment, 2017,599-600:710-720.
[7]	王亚楠, 吕杰. 东北地区玉米和大豆生产成本效率的区域差异及影响因素[J]. 农业经济, 2020, 395(3):12-14.
[8]	WANG Y, XU X, LIU T, et al. Analysis of bacterial and fungal communities in continuous-cropping ramie (Boehmeria nivea L. Gaud) fields in different areas in China[J]. Scientific reports, 2020, 10(1):3564-3569.
[9]	JIE W G, TAN Y W, YANG D Y, et al. Effects of Rhizophagus intraradices and Acinetobacter calcoaceticus on soybean growth and carbendazim residue[J]. Sustainability, 2023, 15(13):10322.
[10]	KUMAWAT K C, RAZDAN N, SAHARAN K. Rhizospheric microbiome: Bio-based emerging strategies for sustainable agriculture development and future perspectives[J]. Microbiological research, 2022, 254:126901.
[11]	MUNIR N, HANIF M, ABIDEEN Z, et al. Mechanisms and strategies of plant microbiome interactions to mitigate abiotic stresses[J]. Agronomy, 2022, 12:2069.
[12]	GAI J P, FAN J Q, ZHANG S B, et al. Direct effects of soil cadmium on the growth and activity of arbuscular mycorrhizal fungi[J]. Rhizosphere, 2018, 7:43-48.
[13]	ZHAO Y, CARTABIA A, LALAYMIA I, et al. Arbuscular mycorrhizal fungi and production of secondary metabolites in medicinal plants[J]. Mycorrhiza, 2022, 32(3-4):221-256. doi: 10.1007/s00572-022-01079-0 pmid: 35556179
[14]	ULLAH U Z. REHMAN A N, ZAHOOR A, et al. Dose optimization of silicon for boosting arbuscular mycorrhizal fungi colonization and cadmium stress mitigation in maize (Zea mays L.)[J]. Environmental science and pollution research international, 2023, 30(25):67071-67086.
[15]	ZENG H L, ZHONG W, TAN F X, et al. The influence of Bt maize cultivation on communities of arbuscular mycorrhizal fungi revealed by MiSeq sequencing[J]. Frontiers in microbiology, 2019, 9:3275.
[16]	MARRO N, COFRE N, GRILLI G, et al. Soybean yield, protein content and oil quality in response to interaction of arbuscular mycorrhizal fungi and native microbial populations from mono- and rotation-cropped soils[J]. Applied soil ecology, 2020, 152:103575.
[17]	ZHANG F G, LIU M H, LI Y, et al. Effects of arbuscular mycorrhizal fungi, biochar and cadmium on the yield and element uptake of Medicago sativa[J]. Science of the total environment, 2019, 655:1150-1158.
[18]	MA X, LUO W, LI J, et al. Arbuscular mycorrhizal fungi increase both concentrations and bioavilability of Zn in wheat (Triticum aestivum L.) grain on Zn-spiked soils[J]. Applied soil ecology, 2018, 135:91-97.
[19]	吴会会, 吴强盛. 盐胁迫下丛枝菌根真菌对枳实生苗生长及生理代谢的影响[J]. 中国南方果树, 2023, 52(6):8-13.
[20]	NOROOZI N, MOHAMMADI G, GHOBADI M. Some physio-biochemical traits of sunflower as affected by arbuscular mycorrhizal fungi inoculation under different irrigation treatments[J]. Italian journal of agronomy, 2023, 18: 2033.
[21]	NAHID M, HADI K, SAEED A. Alleviation of Rhizoctonia root rot damage in common bean by some arbuscular mycorrhizal fungi[J]. Journal of applied research in plant protection, 2023, 12(1):13-24.
[22]	接伟光, 林厚泽, 杨冬莹, 等. 根内根孢囊霉对大豆成熟期生物量、根腐病及根际土壤百菌清残留的影响[J]. 江苏农业科学, 2023, 51(14):153-158.
[23]	宋福强, 程蛟, 常伟, 等. 田间施加AM菌剂对大豆生长效应的影响[J]. 中国农学通报, 2013, 29(6):69-74.
[24]	郭美玲, 郭泰, 王志新, 等. 大豆重迎茬问题及与玉米轮作大豆季整地技术综述[J]. 农业科技通讯, 2023(3):165-168.
[25]	接伟光, 杨冬莹, 姚延轩, 等. 正茬与迎茬对大豆根际土壤微生物群落组成的影响[J]. 大豆科学, 2022, 41(2):179-188.
[26]	李森, 姚钦, 刘俊杰, 等. 大豆重迎茬研究进展[J]. 大豆科学, 2020, 39(2):317-324.
[27]	李伟群. 不同年限连作大豆根际微生物群落及生物量的动态变化研究[D]. 北京: 中国农业科学院, 2011.
[28]	YANG Z, DONG H, ZHANG S, et al. Isolation and identification of mycorrhizal helper bacteria of Vaccinium uliginosum and their interaction with mycorrhizal fungi[J]. Front microbiol, 2023, 14:1-13.
[29]	SANGWAN S, PRASANNA R. Mycorrhizae helper bacteria: Unlocking their potential as bioenhancers of plant-arbuscular mycorrhizal fungal associations[J]. Microbial ecology, 2022, 84(1):1-10.
[30]	宋瑞清, 邓勋, 宋小双. 菌根辅助细菌与外生菌根互作机制研究进展[J]. 吉林农业大学学报, 2016, 36(4):379-384.
[31]	BAI L, CUI J Q, JIE W G, et al. Analysis of the community compositions of rhizosphere fungi in soybeans continuous cropping fields[J]. Microbiological research, 2015, 180:49-56. doi: 10.1016/j.micres.2015.07.007 pmid: 26505311
[32]	LI Y, SHI C, WEI D, et al. Soybean continuous cropping affects yield by changing soil chemical properties and microbial community richness[J]. Microbiol, 2022, 13:1083736.
[33]	SPAGNILETTI F N, LEIVA M, CHIOCCHIO V, et al. Phosphorus fertilization reduces the severity of charcoal rot (Macrophomina phaseolina) and the arbuscular mycorrhizal protection in soybean[J]. Journal of plant nutrition and soil science, 2018, 181:855-860.
[34]	NACOON S, JOGLOY S, RIDDECH N, et al. Combination of arbuscular mycorrhizal fungi and phosphate solubilizing bacteria on growth and production of Helianthus tuberosus under field condition[J]. Scientific reports, 2021, 11(1):6501. doi: 10.1038/s41598-021-86042-3 pmid: 33753844

土壤类型	AM真菌孢子密度/（个/g）
正茬(0y)	5.3±0.6
迎茬(1y)	2.4±0.4

土壤类型	AM真菌孢子密度/（个/g）
正茬(0y)	5.3±0.6
迎茬(1y)	2.4±0.4

处理方式	30 d	60 d	90 d	120 d
0yInN	12.05±0.98^b	38.21±1.26^a	59.48±1.58^a	90.25±2.13^a
0yNonN	5.13±0.63^c	11.09±1.02^c	20.98±1.19^c	28.73±1.56^c
0yCkN	5.20±0.75^c	10.85±0.96^c	22.06±1.33^c	26.02±1.37^c
0yInM	10.97±1.02^b	30.45±1.41^b	51.32±1.52^b	85.41±1.98^b
1yInN	14.26±1.21^a	42.19±1.52^a	64.72±1.84^a	93.53±2.35^a
1yNonN	6.92±0.56^c	13.06±0.51^c	24.47±1.19^c	32.85±1.52^c
1yCkN	6.84±0.81^c	12.91±0.87^c	26.39±1.45^c	30.34±1.47^c
1yInM	12.26±1.12^b	34.52±1.46^b	55.33±1.58^b	89.76±1.75^a

处理方式	30 d	60 d	90 d	120 d
0yInN	12.05±0.98^b	38.21±1.26^a	59.48±1.58^a	90.25±2.13^a
0yNonN	5.13±0.63^c	11.09±1.02^c	20.98±1.19^c	28.73±1.56^c
0yCkN	5.20±0.75^c	10.85±0.96^c	22.06±1.33^c	26.02±1.37^c
0yInM	10.97±1.02^b	30.45±1.41^b	51.32±1.52^b	85.41±1.98^b
1yInN	14.26±1.21^a	42.19±1.52^a	64.72±1.84^a	93.53±2.35^a
1yNonN	6.92±0.56^c	13.06±0.51^c	24.47±1.19^c	32.85±1.52^c
1yCkN	6.84±0.81^c	12.91±0.87^c	26.39±1.45^c	30.34±1.47^c
1yInM	12.26±1.12^b	34.52±1.46^b	55.33±1.58^b	89.76±1.75^a

处理方式	30 d	60 d	90 d	120 d
0yInN	7.33±0.85^a	58.13±0.95^a	87.69±1.16^a	101.12±1.23^a
0yNonN	6.23±0.93^b	52.47±1.02^c	72.57±1.09^c	87.23±1.14^c
0yCkN	6.42±0.58^b	51.56±0.78^c	74.82±0.97^c	85.82±1.03^c
0yInM	7.28±0.91^a	55.58±0.93^b	80.79±1.23^b	95.35±1.17^b
0yNonM	6.12±0.68^bc	50.42±1.02^cd	69.35±1.08^cd	80.86±1.22^d
0yCkM	6.04±0.72^bc	49.01±0.63^cd	71.92±1.64^cd	81.19±1.31^d
1yInN	6.55±0.76^b	53.29±0.75^b	76.33±0.87^c	89.27±1.09^c
1yNonN	5.85±0.68^c	48.37±0.73^d	62.25±1.12^d	71.42±1.28^de
1yCkN	5.68±0.52^c	47.63±0.65^d	61.71±1.26^d	70.33±1.41^de
1yInM	6.37±0.60^b	51.27±0.58^c	70.39±1.47^c	82.86±1.39^cd
1yNonM	5.51±0.77^c	45.33±0.49^e	58.72±1.05^e	65.39±1.51^e
1yCkM	5.31±0.69^c	44.82±0.52^e	56.99±1.03^e	64.28±1.37^e

根内根孢囊霉对正茬和迎茬大豆生物量的影响

Effects of Rhizophagus intraradices on Biomass of Rotational Cropping and Alternate Cropping Soybean

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处理方式	30 d	60 d	90 d	120 d
0yInN	2.68±0.32^a	13.84±0.75^a	27.40±1.02^a	30.61±1.27^a
0yNonN	2.29±0.27^b	12.49±0.83^b	22.68±0.87^c	26.43±1.08^c
0yCkN	2.35±0.21^b	12.28±0.70^b	23.19±0.52^c	26.01±1.13^c
0yInM	2.60±0.33^a	13.20±0.61^a	25.25±1.03^b	28.5±1.31^b
0yNonM	2.24±0.30^b	12.03±0.53^b	21.67±0.85^c	24.62±1.04^d
0yCkM	2.21±0.19^b	11.86±0.72^c	22.23±0.79^c	24.58±1.35^d
1yInN	2.39±0.26^b	12.69±0.51^b	23.85±0.68^c	27.06±1.24^c
1yNonN	2.14±0.22^c	11.52±0.62^c	19.45±1.03^d	21.52±1.09^e
1yCkN	2.08±0.31^c	11.33±0.59^c	19.28±0.86^d	21.21±1.21^e
1yInM	2.30±0.18^b	12.02±0.42^b	21.98±0.78^c	25.11±1.30^c
1yNonM	2.02±0.33^d	10.79±0.66^d	18.25±0.94^e	19.82±1.17^f
1yCkM	1.95±0.27^d	10.67±0.46^d	17.97±0.82^e	19.49±1.02^f

处理方式	30 d	60 d	90 d	120 d
0yInN	0.85±0.08^a	6.91±0.68^a	10.55±0.72^a	14.87±0.89^a
0yNonN	0.64±0.05^b	6.25±0.72^b	8.73±0.68^b	12.83±0.92^b
0yCkN	0.69±0.10^b	6.17±0.56^b	9.00±0.70^b	12.62±0.75^b
0yInM	0.80±0.03^a	6.61±0.77^ab	9.72±0.69^ab	14.02±1.03^a
0yNonM	0.58±0.07^bc	6.01±0.71^b	8.36±0.81^b	11.89±0.95^c
0yCkM	0.62±0.04^bc	5.86±0.68^b	8.61±0.76^b	11.93±0.88^c
1yInN	0.73±0.08^b	6.34±0.56^b	9.19±0.62^b	13.12±1.06^b
1yNonN	0.52±0.06^c	5.76±0.69^c	7.49±0.59^c	10.50±0.97^d
1yCkN	0.54±0.09^c	5.67±0.52^c	7.42±0.53^c	10.34±0.83^d
1yInM	0.63±0.09^b	6.10±0.59^b	8.47±0.63^b	12.18±0.78^bc
1yNonM	0.49±0.07^c	5.38±0.63^d	7.06±0.62^d	9.61±0.91^e
1yCkM	0.50±0.03^c	5.33±0.79^d	6.85±0.71^d	9.45±0.80^e

处理方式	30 d	60 d	90 d	120 d
0yInN	0.32±0.04^a	2.55±0.29^a	3.49±0.46^a	4.23±0.71^a
0yNonN	0.24±0.05^b	2.31±0.30^c	2.89±0.38^b	3.65±0.58^b
0yCkN	0.26±0.03^b	2.28±0.23^c	2.98±0.50^b	3.59±0.65^b
0yInM	0.30±0.05^a	2.44±0.19^b	3.21±0.41^ab	3.99±0.62^ab
0yNonM	0.22±0.07^c	2.22±0.25^d	2.76±0.38^b	3.38±0.72^c
0yCkM	0.23±0.04^c	2.17±0.31^d	2.85±0.29^b	3.39±0.50^c
1yInN	0.27±0.03^b	2.34±0.34^c	3.04±0.49^b	3.73±0.47^b
1yNonN	0.19±0.04^d	2.13±0.19^d	2.48±0.36^c	2.99±0.61^d
1yCkN	0.20±0.06^d	2.10±0.27^d	2.45±0.29^c	2.94±0.59^d
1yInM	0.23±0.05^c	2.26±0.30^c	2.80±0.42^b	3.47±0.43^bc
1yNonM	0.18±0.04^d	1.99±0.18^e	2.33±0.36^d	2.73±0.63^e
1yCkM	0.18±0.03^d	1.97±0.15^e	2.26±0.40^d	2.69±0.47^e

处理方式	30 d	60 d	90 d	120 d
0yInN	31.32±0.68^a	54.32±1.06^a	70.22±2.06^a	86.22±1.85^a
0yNonN	30.87±0.52^b	49.51±1.34^c	61.32±2.29^c	74.17±1.25^c
0yCkN	30.23±0.31^b	50.82±1.28^c	62.95±1.76^c	72.46±1.83^c
0yInM	30.07±0.66^b	52.84±1.52^b	67.53±1.38^b	83.35±2.05^b
0yNonM	29.75±0.41^c	47.37±1.03^d	58.93±1.42^d	71.68±1.37^cd
0yCkM	29.43±0.73^c	48.93±1.96^d	59.37±2.15^d	70.37±1.92^cd
1yInN	30.46±0.84^b	50.13±1.67^c	63.56±2.37^bc	76.29±1.47^c
1yNonN	29.84±0.78^c	45.32±2.03^e	56.29±1.86^e	69.17±2.03^d
1yCkN	29.64±0.63^c	44.82±1.28^e	54.95±1.73^e	68.94±1.81^d
1yInM	29.81±0.47^c	47.26±1.37^d	61.31±1.80^c	74.86±1.28^c
1yNonM	29.23±0.31^c	43.64±1.29^f	53.17±1.56^f	67.62±2.24^e
1yCkM	29.38±0.42^c	42.96±1.15^f	52.88±1.67^f	66.91±1.35^e

处理方式	30 d	60 d	90 d	120 d
0yInN	0.37±0.09^a	0.54±0.11^a	0.79±0.13^a	1.04±0.07^a
0yNonN	0.34±0.08^c	0.48±0.08^c	0.70±0.17^c	0.89±0.08^c
0yCkN	0.33±0.06^c	0.47±0.07^c	0.69±0.16^c	0.86±0.07^c
0yInM	0.35±0.11^b	0.51±0.10^b	0.73±0.11^b	0.99±0.06^b
0yNonM	0.32±0.05^cd	0.46±0.09^cd	0.67±0.12^cd	0.84±0.05^cd
0yCkM	0.32±0.08^cd	0.45±0.05^cd	0.68±0.10^cd	0.83±0.07^d
1yInN	0.34±0.10^c	0.50±0.11^b	0.71±0.09^c	0.93±0.06^c
1yNonN	0.31±0.05^d	0.43±0.07^d	0.64±0.07^d	0.81±0.04^d
1yCkN	0.30±0.09^d	0.44±0.06^d	0.63±0.05^d	0.82±0.06^d
1yInM	0.32±0.08^cd	0.47±0.12^c	0.68±0.04^cd	0.90±0.07^c
1yNonM	0.29±0.03^d	0.42±0.08^d	0.62±0.03^d	0.79±0.06^d
1yCkM	0.29±0.04^d	0.41±0.03^d	0.61±0.06^d	0.80±0.05^d

	30 d	60 d	90 d	120 d
0yInN	12.06±0.20a	17.32±0.19a	20.16±0.32a	24.03±0.25a
0yNonN	10.23±0.19c	14.35±0.16c	16.51±0.29c	19.78±0.31c
0yCkN	10.35±0.15c	13.78±0.21c	15.97±0.15c	18.26±0.19c
0yInM	11.39±0.18b	15.71±0.08b	18.86±0.18b	22.15±0.27b
0yNonM	10.09±0.12c	13.02±0.11d	14.57±0.23d	16.89±0.15d
0yCkM	10.33±0.08c	13.29±0.21d	14.09±0.27d	17.37±0.22d
1yInN	11.12±0.21b	15.01±0.10bc	17.15±0.18bc	21.09±0.21bc
1yNonN	9.87±0.13d	12.86±0.09de	14.31±0.34d	16.75±0.17d
1yCkN	9.69±0.17d	12.42±0.13de	14.22±0.17d	15.79±0.13d
1yInM	10.73±0.14bc	14.88±0.25c	16.42±0.25c	19.72±0.33c
1yNonM	9.29±0.06e	12.17±0.18e	13.28±0.14e	14.81±0.24e
1yCkM	8.95±0.09e	11.03±0.17e	12.97±0.26e	13.62±0.19e

处理方式	百粒重/g
0yInN	26.87±1.12^a
0yNonN	24.32±1.07^c
0yCkN	24.16±1.20^c
0yInM	25.93±1.28^b
0yNonM	23.71±0.96^cd
0yCkM	23.94±1.18^cd
1yInN	22.34±1.19^d
1yNonN	19.23±0.85^e
1yCkN	18.83±0.77^e
1yInM	21.68±1.01^d
1yNonM	17.73±1.12^f
1yCkM	17.98±0.63^f

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