转录组测序技术在甘薯研究中的应用

doi:10.11924/j.issn.1000-6850.casb2023-0375

中国农学通报 ›› 2024, Vol. 40 ›› Issue (30): 94-102.doi: 10.11924/j.issn.1000-6850.casb2023-0375

• 资源·环境·生态·土壤 • 上一篇下一篇

转录组测序技术在甘薯研究中的应用

郎涛¹(), 杨飞洋¹^,², 蒲志刚¹, 张聪¹, 李明¹, 余马², 屈会娟¹, 伍竞宇¹, 张璐¹, 刁杜¹, 冯俊彦¹()

¹ 四川省农业科学院生物技术核技术研究所，成都 610066
² 西南科技大学生命科学与工程学院，四川绵阳 621010

收稿日期:2023-05-10 修回日期:2024-03-30 出版日期:2024-10-23 发布日期:2024-10-23
通讯作者:
冯俊彦，男，1985年出生，甘肃天水人，副研究员，博士，主要从事农作物生物技术育种研究。通信地址：610066 四川省成都市锦江区狮子山路106号四川省农业科学院生物技术核技术研究所，Tel：028-84543128，E-mail：275735548@qq.com。
作者简介:
郎涛，男，1987年出生，河南济源人，助理研究员，博士，主要从事生物信息学和分子细胞遗传学研究。通信地址：610066 四川省成都市锦江区狮子山路106号四川省农业科学院生物技术核技术研究所，Tel：028-84543128，E-mail：langtao123xxx@126.com。
基金资助:
四川省财政自主创新专项“甘薯高产遗传位点鉴定及高产优异种质挖掘”(2022YXLW003); 四川省农业科学院现代农业学科建设推进工程“菜用甘薯新材料创制及工厂化生产技术研发”(2021XKJS061); 四川省农业科学院战略科学家工作室项目(2023ZLKXJGZS001); 四川省科技支撑计划“四川省主要粮油作物分子育种平台”(2021YFY0027)

Application of Transcriptome Sequencing Technology in Sweetpotato (Ipomoea batatas L.) Research

LANG Tao¹(), YANG Feiyang¹^,², PU Zhigang¹, ZHANG Cong¹, LI Ming¹, YU Ma², QU Huijuan¹, WU Jingyu¹, ZHANG Lu¹, DIAO Du¹, FENG Junyan¹()

¹ Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066
² School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010

Received:2023-05-10 Revised:2024-03-30 Published:2024-10-23 Online:2024-10-23

摘要/Abstract

摘要：

甘薯是世界上重要的粮食、饲料、工业原料作物。随着高通量测序技术的快速发展，转录组测序技术已广泛应用于甘薯研究之中。为了今后利用转录组学技术更好地开展甘薯功能基因挖掘、重要性状调控机制解析等研究，本综述分析了各国研究机构共享的甘薯转录组数据，并总结了近年来转录组测序技术在甘薯逆境胁迫响应、块根发育和淀粉合成等方面的应用及取得的成果。认为目前甘薯转录组研究在试验设计、数据挖掘以及研究结果实用性方面仍存在不足。提出在未来研究中，可将转录组学技术与其他组学技术及遗传学分析方法相结合，以推动甘薯研究的不断深入。

关键词: 甘薯, 转录组, 生长发育, 逆境响应, 品质形成, 高通量测序, 块根发育, 淀粉合成

Abstract:

Sweetpotato is an important food, fodder and industrial raw material crop in the world. With the rapid development of high-throughput sequencing technologies, transcriptome sequencing technology has been widely used in sweetpotato studies. To better carry out research on functional gene mining and important trait regulation mechanisms analyzing in sweetpotato using transcriptomics techniques in the future, this study analyzed the profile of sweetpotato transcriptome data shared by research institutions in various countries, and summarized the applications and achievements of transcriptome sequencing technology in researches on sweetpotato over recent years, from the aspects of response to adversity stress, tuberous root development and starch synthesis. It was pointed out that there were still deficiencies in experimental design, data mining, and practicality of research results in current sweetpotato transcriptome studies. It was proposed that in further studies, transcriptomics technologies should be integrated with other omics technologies and genetic analysis methods to promote the continuous deepening of sweetpotato research.

Key words: sweetpotato, transcriptome, growth and development, stress resistance, quality formation, high-throughput sequencing, tuberous root development, starch synthesis

郎涛, 杨飞洋, 蒲志刚, 张聪, 李明, 余马, 屈会娟, 伍竞宇, 张璐, 刁杜, 冯俊彦. 转录组测序技术在甘薯研究中的应用[J]. 中国农学通报, 2024, 40(30): 94-102.

LANG Tao, YANG Feiyang, PU Zhigang, ZHANG Cong, LI Ming, YU Ma, QU Huijuan, WU Jingyu, ZHANG Lu, DIAO Du, FENG Junyan. Application of Transcriptome Sequencing Technology in Sweetpotato (Ipomoea batatas L.) Research[J]. Chinese Agricultural Science Bulletin, 2024, 40(30): 94-102.

图/表 2

国家	品种
巴巴多斯	CBS-32
巴布亚新几内亚	PI 564154 (KEANJA)
菲律宾	PI 585108 (TAINUNG 68)
乌干达	Tanzania
新喀里多尼亚	PI 318848 (Kamula Belep)
以色列	Georgia Jet
印度	PI 585061 (KALMEGH S-30)
汤加	PI 573318 (AMELIKA)、PI 585054 (HALASIKA)
秘鲁	PI 531122 (JEWEL)、PI 599387 (Jonathan)、PI 612701 (MAN LOBOR)
韩国	Juhwangmi、PI 585067 (OKINAWA 100)、Sinhwangmi、Yulmi
日本	Annouimo、Beniharuka、Kokei 14、Kyusyu166、Tamayutaka、PI 344123 (NANYO)、PI 595868 (BENIHAYATO)
美国	Beauregard、Hatteras、Nugget、PI 208886 (527)、PI 566617 (CAROMEX)、PI 566638 (JEWEL)、PI 592993 (PERLA)、PI 634379 (HAYMAN)、SC1149-19
中国	15-94、川薯218、广薯87、京薯6号、金山57、KT1、栗子香、漯徐薯8号、高自1号、台农57、新种花、徐薯18、郑薯20、ND98、HSCL8、日节成、苏薯8号、济薯26、农林54、徐薯15、徐薯22、徐薯32、福薯7-6、EC16、广薯146、宁紫1号、徐紫菜8、济薯25、济薯29、泰中6号、徐紫薯3号、浙薯81、湘薯99、浙紫薯1号、川山紫、济紫薯2号、济紫薯3号、济紫薯4号

表1. NCBI-SRA中转录组测序的甘薯品种统计

图1. 国内外科研机构在NCBI-SRA数据库中上传的甘薯转录组数据统计 Z01：中国科学院上海生物化学与细胞生物学研究所；Z02：江苏师范大学；Z03：山东省农业科学院；Z04：河南省农业科学院；Z05：四川大学；Z06：广西大学；Z07：西南科技大学；Z08：福建农林大学；Z09：湖南理工学院；Z10：南昌大学；Z11：中国科学院大学；Z12：中国农业大学；Z13：南方科技大学；Z14：广东省农业科学院作物研究所；Z15：徐州市农业科学院；Z16：海南大学；Z17：湖北省农业科学院；Z18：山西农业大学；Z19：台湾大学；M01：康奈尔大学；M02：密歇根州立大学；M03：密歇根大学；M04：西弗吉尼亚州立大学；M05：阿肯色大学松崖分校；M06：东卡罗莱纳大学；M07：塔斯基吉大学；M08：印第安纳大学；H01：韩国生物科学与生物技术研究所；H02：庆尚大学；H03：首尔大学；H04：首尔市立大学；R01：KARC/NARO；R02：冈山大学；R03：近畿大学；Y01：ARO-VOLCANI；X01：CNB-CSIC；A01：昆士兰科技大学；F01：洛林大学

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转录组测序技术在甘薯研究中的应用

Application of Transcriptome Sequencing Technology in Sweetpotato (Ipomoea batatas L.) Research

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