RNA干扰及其植物抗病毒应用

摘要/Abstract

摘要： 【研究目的】RNA干扰(RNA interference，RNAi)是分子生物学研究领域的一项新兴技术，在动植物的遗传改良、功能基因组、基因治疗等研究中广泛应用；【方法】它是指一些小的双链RNA分子介导的、序列特异的转录后基因沉默现象；【结果】本文就RNA干扰的作用机制、RNA干扰与植物病毒间的互作以及RNA干扰表达载体构建的一些研究进展进行了综述；【结论】随着RNAi研究的进一步深入，RNAi必将成为遗传育种工作者培育抗病毒植物的有力手段，促进植物抗病育种的发展。

关键词: 小麦, 小麦, 籽粒硒含量, 富硒技术

Abstract: 【Objective】RNA interference is new technique in molecular biological research. It had been widely used in the research of animal and plant for genetic improvement, functional genome and gene therapy;【Method】RNAi is a post-transcriptional gene-silencing phenomenon induced by double-stranded RNA.【Results】In this paper, the mechanism of RNA interference, the interaction of RNA interference and plant virus and the construction of RNAi expression vector were reviewed;【Conclusion】With further study on it, RNAi will become a powerful skill in plant anti-virus breeding.

中图分类号:

Q752

张恭,刘立峰,马峙英. RNA干扰及其植物抗病毒应用[J]. 中国农学通报, 2007, 23(1): 42-042.

Zhang Gong, Liu Lifeng, Ma Zhiying. RNA Interference and its Application in Plant anti-virus[J]. Chinese Agricultural Science Bulletin, 2007, 23(1): 42-042.

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