植物抗病毒基因工程研究进展

摘要/Abstract

摘要： 简要讨论了近年来植物抗病毒基因工程的方法策略，主要包括：植物自身的抗病毒基因策略、来源于病毒的抗性基因策略,干扰素等抗性策略；并分析了其存在问题和发展趋势。

关键词: 辣椒疫病, 辣椒疫病, 水杨酸, 诱导抗性, 木质素, 多酚氧化酶, 过氧化物酶, 苯丙氨酸解氨酶

Abstract: The paper briefly discussed the strategies in genetic engineering for plant resistance to viral diseases in recent years. These strategies include host-gene-mediated resistance strategies and pathogen-derived resistance strategies, which include viral coat protein gene, movement protein gene, replicase gene, satellite RNA, antisense RNA, and defective interfering RNA. In addition, antibody gene, ribozyme and in terferon also have the ability to confer resistance against plant viruses. Meanwhile, resistance mechanisms and the perspective of application of each strategy were briefly discussed.

吴祥华,胡宗利,陈国平. 植物抗病毒基因工程研究进展[J]. 中国农学通报, 2007, 23(1): 46-046.

Wu Xianghua, Hu Zongli, Chen Guoping. Research Advances in Genetic Engineering of Resistance to Plant Virus[J]. Chinese Agricultural Science Bulletin, 2007, 23(1): 46-046.

参考文献

[1] Hamilton, R I. Defense triggered by previous invaders: Viruses. In: Horsefall J G, cowlingE B.Plant Disease: An Advanced Treatise 5.New York: Academic Press,1980:279-303.
[2] Baker B, Zambryski P, Staskawicz B, et al. Singaling in plant Microbe Interactions. Science,1997,276:726-733.
[3] Dangl J L.Plant pathogens and integrated defence responses to infection. .Nature,2001,411:826-833.
[4] 姜国勇,杨仁崔.番茄Tm-22基因在烟草中的表达及其对番茄花叶病毒(ToMV)的特异抗性[J].病毒学报,2003,19(4):365-370.
[5] Flor H H. Current status of the gene-for-gene concept.Ann Rev Phytopathol, 1971,9:275-296.
[6] Frank C.Lanfermeijer. The products of the broken Tm-2 and the durable Tm-22 resistance genes from tomato differ in four amino acids.Journal of Experimental Botany,2005,(9):1-9.
[7] Powell Abel P, Nelson R S, De B, et al. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene [J].Science,1986,232:738-743.
[8] 张海燕,陈正华.获得病毒抗性的植物基因工程策略及其抗病机理研究进展[J].武汉植物学研究,1997,17(增刊):15-24.
[9] Wisniewski L A, Powell P A. Local and systemic spread of tobacco mosaic virus in transgenic tobacco plant. The Plant Cell,1990,(2):559-567.
[10] Golemboski D B, Lomonossoff G P, Zaitlin M, et al. Plants transformed with tobacco mosaic virus nonstructural gene sequence are resistant to the virus.Proc Natl Acad Sci USA,1990,87:6311-6315.
[11] 陈谷,等.植物抗病毒基因工程的研究进展.生物技术通报,1999,(6):17-22.
[12] Lomonossoff. Pathogen derived resistance to plant viruses. Ann Rev Phytopathol, 1995,33:323-343.
[13] Suzuki M, Masuta C, Takanami Y, et al. Resistance against cucumber mosaic virus in plants expressing the viral replicon.FEBS Lett,1993,379:26-30.
[14] Cooper B, Lapldot M, Heick J A, et al. A defective movement protein of TMV in transgenic plants confers resistance to multiple viruses whereas the functional analog increases susceptibility.Virology,1995,206:307-313.
[15] Ziegler-Graff V, Guilford P J, Baulcombe D C. Tobacco rattle virus RNA-129K gene product potentates viral movement and also affects symptom induction in tobacco.Virology,1991,182:145-155.
[16] Baulcombe D C, Saunders G R, Revan M W, et al. Expression of biologically active viral satellite RNA from the nuclear genome of transformed plants.Nature,1986,321:446.
[17] 王苏燕,等.转基因油菜中核酶介导的对花椰菜花叶病毒的高度抗病性.中国科学,1997,27:426-431.