羊草乙醛脱氢酶（ALDH）基因片段的克隆及表达分析

摘要/Abstract

摘要： [研究目的]克隆羊草的乙醛脱氢酶ALDH基因片段，研究该基因在不同条件下的表达情况。[方法]采用RT-PCR技术克隆羊草的ALDH基因片段，并对其核苷酸和氨基酸序列进行分析，采用Real Time RT-PCR 方法研究该基因的表达。[结果]获得了羊草的ALDH基因片段，长度为675 bp，编码225aa。核苷酸序列比较表明，与水稻ALDH1a序列(AB037421)同源性为86%，与玉米RF2C（AF348413）同源性为85%。BLASTp分析，该序列与水稻、玉米、拟南芥的乙醛脱氢酶一致性分别高达87%、86%、60%，含有醛脱氢酶基因家族的保守结构域。Real Time RT-PCR数据表明，在诱导条件下该基因的表达量呈先升高后降低的趋势。总体上来说，该基因对盐的响应要高于干旱和冷冻。[结论]本研究成功获得了羊草ALDH基因片段，并研究了该基因的表达情况，为进一步克隆羊草ALDH全长基因奠定了基础。

关键词: 神舟四号, 神舟四号, 玉米自交系, 空间条件, 农艺性状, 变异

Abstract: 【Objective】Aldehyde Dehydrogenase (ALDH) gene segment from Leymus Chinensis was cloned and the expression of it was researched under the different situations.【Method】ALDH gene segment was cloned by RT-PCR. The nucleotide and amino acid sequence of ALDH were analyzed. The expression of ALDH was researched by Real Time RT-PCR.【Result】ALDH gene segment was obtained which was 675bp and coding 225aa. The compare of nucleotide sequences indicated that it has 86% identity with rice ALDH1a (AB037421), and 85% identity with maize RF2C（AF348413）. By BLASTp Analysis, it shares 87%, 86% and 60% amino acid sequence identity with rice, maize and Arabidopsis thaliana ALDH, respectively, which containing conservation region of aldehyde dehydrogenase families. The data of Real Time RT-PCR indicated that the expression of ALDH was increased first, and then decreased under the inducing situations. As a whole, the responses of ALDH to salt and drought were much higher than to cold.【Conclusion】In this research, ALDH gene segment was obtained, and we researched the expression of ALDH. It established basis on cloning the span of ALDH in Leymus Chinensis.

中图分类号:

Q786

李新玲,,杨传平,徐香玲. 羊草乙醛脱氢酶（ALDH）基因片段的克隆及表达分析[J]. 中国农学通报, 2007, 23(6): 115-115.

Li Xinling,, Yang Chuanping, Xu Xiangling. Molecular Cloning and Expression Analysis of Aldehyde Dehydrogenase (ALDH) Gene Segment in Leymus Chinensis[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 115-115.

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