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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (7): 88-88.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

莴苣花叶病毒北京分离物基因组3′末端序列分析

尚巧霞,,向海英,韩成贵,李大伟,于嘉林   

  • 出版日期:2007-07-05 发布日期:2007-07-05

Sequence Analysis of the 3' Terminal Region of the Genome of Lettuce mosaic virus Beijing Isolate

Shang Qiaoxia,, Xiang Haiying, Han Chenggui, Li Dawei, Yu Jialin   

  • Online:2007-07-05 Published:2007-07-05

摘要： 笔者通过RT-PCR方法对LMV北京分离物(LMV-BJ)基因组3′端1620nt的核苷酸片段进行了克隆和序列分析（GenBank登录号为EF423619）。所获片段含有NIb 基因3′端的574nt，编码NIb C-端190个氨基酸；完整的CP基因，全长为834nt，编码一个由277个氨基酸组成的分子量约为30kDa的结构蛋白；3′非编码区含有209nt。通过序列分析软件将LMV-BJ与已经报道的法国分离物O(X97704)、法国分离物E(X97705），美国分离物(X65652)，巴西分离物(AJ278854)和余杭分离物(AJ306288)基因组3′末端和CP基因的核苷酸序列与氨基酸序列分别进行了比较

关键词: 设施农业, 设施农业, 日光温室, 有机质

Abstract: The 3′ terminal genomic 1620 nucleotides of the Lettuce mosaic virus Beijing isolate (LMV-BJ) from China was determined (the GenBank accession number is EF423619). The sequence started within a single open reading frame which was expected to encode a part of the C-terminus of the NIb protein of 190 amino acids by 574 nucleotides, complete capsid protein (CP) of 277 amino acids encoded by 834 nucleotides and followed by a 3′ untranslated region (UTR) of 209 nucleotides. The nucleotide and amino acid sequence identity of 3′ terminal regions and cp gene among LMV-BJ and other five previously reported isolates from France O(X97704), France E(X97705), USA(X65652), Brazil(AJ278854), Yuhang(AJ306288)were compared.

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