棉花生长素应答因子ARF3功能的初步分析

摘要/Abstract

摘要： 生长素应答因子(auxin response factor，ARF)是转录因子的一个家族，在生长素信号传导途径中起着关键的作用。通过GenBank上登录的序列设计引物，利用RT-PCR技术扩增得到了陆地棉生长素应答因子ARF3的全长序列，并构建到植物表达载体pCAMBIA1301上，借助真空渗透转化技术，在农杆菌GV3101介导下转化模式植物拟南芥，经潮霉素筛选，得到了批量的转基因植株。通过PCR技术，检测到目的基因成功整合到拟南芥基因组中，RT-PCR检测到了目的基因在转基因植株中得到表达。

关键词: PGPR, PGPR, 水稻, 产量, 根系活力, 灌浆速率

Abstract: Auxin response factors (ARFs), a family of transcription factors, have been discovered recently. It plays an important roles in auxin signal transduction pathway. We have successfully got the whole sequence of cotton ARF3 through RT-PCR, and transferred it to the plant expression vector,pCAMBIA1301.By Vacuum infiltration technology ,the plant expression vector has been transmitted into Arabidoposis thaliana. We have got planty of transgentic plants. The PCR detection proves the sequnece has successfully recombined with the genome of Arabidoposis thaliana.We also prove its expression by the method of RT-PCR.

中图分类号:

张锋,崔百明. 棉花生长素应答因子ARF3功能的初步分析[J]. 中国农学通报, 2007, 23(7): 84-84.

Zhang feng, Cui Baiming. The primary Function Analysis of the Cotton Auxin Response Factor[J]. Chinese Agricultural Science Bulletin, 2007, 23(7): 84-84.

参考文献

[1] Friml J. Auxin transport-shaping the plant. Curr Opin Plant Biol, 2003,6(1):7-12.
[2] Benkova E, Michniewicz M, Sauer, et al. efflux-dependent auxin gradients as a common module for plant organ formation. Cell, 2003,115(5):591-602.
[3] Reinhardt D, Pesce ER, Stieger P, et al. Regulation of phyllotaxis by polar auxin transport. Nature, 2003,426(6964):255-260.
[4] Mattsson J, Ckurshumova W, Berleth T. Auxin signaling in Arabidopsis leaf vascular development. Plant Physiol, 2003,131:1327-1339.
[5] Blakeslee J J, Peer W A, Murphy A S. Auxin transport. Curr Opin Plant Biol, 2005,8(5):494-500.
[6] Dolan L, Linstead P, Kidner C et al. Cell fate in plants. Lessons from the Arabidopsis root. Symp Soc Exp Biol, 1998,51:11-7.
[7] Friml J, Palme K. Polar auxin transport--old questions and new concepts? Plant Mol Biol, 2002,49(3~4):273-84.
[8] 吴蓓,吴建勇,蔡刘体,等.生长素反应因子.植物生理学通讯,2005,(6):273-277.
[9] John M E. Genetic engineering strategies fox cotton fiber modification. In: Basra A S ed. Cotton fibers: developmental biology, quality improvement,and textile processing. New York: Food Products Press, 1999:271-292.
[10] Kim J, Harter K, Theologis A. Protein-protein interactions among the Aux/IAA proteins. Proc Natl Acad Sci USA 1997,94:11786-11791.
[11] Tatematsu, K., Kumagai, S., Muto, H et al. MASSUGU2 encodes Aux/IAA19, an auxin-regulated protein that functions together with the transcriptional activator NPH4/ARF7 to regulate differential growth responses of hypocotyl and formation of lateral roots in Arabidopsis thaliana. Plant Cell, 2004,16:379-393.
[12] Robert W, Seagull et al. Pre- and Post-Anthesis Application of Exogenous Hormones Alters Fiber Production in Gossypium hirsutum L. Cultivar Maxxa GTO. The Journal of Cotton Science, 2004,8:105-111.
[13] Suo J F, Liang X E, LI Pu, Zhang Y S et al. Identification of GhMYB109 encoding a R2R3 MYB transcription factor that expressed specially in fiber initials and elongating fibers of cotton(Gossypium hirsutum L). Biochim Biophys Acta, 2003,1630(1):25-34.

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