大豆脂氧酶分析鉴定技术的研究进展及在育种中的应用

摘要/Abstract

摘要： 大豆脂肪氧化酶分析鉴定技术是将大豆种子中分离与纯化的脂肪氧化同工酶，利用遗传分析、免疫、色谱分析、显色等方法进行定性或定量的分析，检测品种的脂氧酶缺失类型。随着无腥味大豆育种研究的深入，现已育成品种的商品化生产，食品加工业的蓬勃发展，对大豆脂肪氧化酶鉴定技术要求不断提高和改进。笔者综述了大豆脂肪氧化同工酶的生物特性、遗传学特点及其各种分析鉴定技术的优缺点、灵敏度和适应范围，并且认为，在比较分析的基础上，以选择有益材料培育新种质、新品种的辅助育种和食品加工的鉴定为目的，使用最经济有效的检测手段是快速准确筛选缺失品种的合理策略。进一步讨论了大豆脂肪氧化酶分析鉴定技术运用于辅助育种所存在的问题和应用前景

Abstract: Analysis and detection methods for Soybean Lipoxygenase is to test Lipoxygenase-free types of Soybean, which is isolated and extracted in Soybean seed, through Genetical analysis, Immunoassay, Chromatography, colorimetric assays and so on. As following Soybean breeding researched deeply, and breed of absent flavor of tofu and soymilk selled widely, and soybean production industry advanced prosperously, it is about to improve identification test in soybean lipoxygenase increasingly. In this paper, biologic and genetic characteristics about soybean lipoxygenase, the advantages and disadvangtages, sensitivity and applicability of identification test are summarized. The logical strategy, based on the current development status, would be the best economical and accurate method employed in considering large-scale detection in order to breeding. the problem and application perspective of Analysis and detection methods for Soybean Lipoxygenase are also discussed in breeding selection early and seed identification in industry.

中图分类号:

S565.1

刘渊,张孟臣,张彩英,马峙英. 大豆脂氧酶分析鉴定技术的研究进展及在育种中的应用[J]. 中国农学通报, 2007, 23(7): 101-101.

Liu Yuan, Zhang Mengchen, Zhang Caiying, Ma Zhiying. Progress of Analysis and Detection Methods for Soybean Lipoxygenase and Application in Genetics Breeding[J]. Chinese Agricultural Science Bulletin, 2007, 23(7): 101-101.

参考文献

[1] 王金龙,李星华,陈宛妹.大豆脂肪氧化酶与籽粒营养品质关系的研究.中国粮油学报,2000,15(3):36-38.
[2] Andre E, Hou K.W..Sur la presence dcune oxydase des lipids ou lipoxydase dans la grain de so ja, glycine so ja life. Compte Rendu Acad Sci(paris), 1932,194:645-647.
[3] Sessa D J, Rackis J J. Lipid-derived flavors of legume protein products.J. Am Oil Chem.Soci, 1977,54:468-473.
[4] Moreira M A, Tavares S R, Ramos V, et al. Hexanal production and TBA number are reduced in soybeanseeds lacking lipoxygenase isoenzymes Z and 3. Agri Food chem, 1993,41(1):103-106.
[5] 杨淑媛,.新编大豆食品.北京:中国商业出版社,1989:45-60.
[6] Kitamura K, Davies C S, Kaizuma N, et al. Genetic analysis of a null-allele for lipoxygenase-3 in soybean seeds. Crop Science, 1983,58:583-586.
[7] Kitamura K, Kumagai T, Kikuchi A. Inheritance of lipoxygenase-2 and genetic relationships among genes for lipoxygenase-1,-2 and -3 isoenzymes in soybean seeds J Breed, 1985,35:413-420.
[8] Pfeiffer T W , Hildebrand D F ,Orf J H. Inheritance of a lipoxygenase-1 allozyme in soybean. Crop Sci, 1993,33:691-693
[9] Davies C S , Nielsen N C. Genetic analysis of a null-allele for lipoxygense-2 in soybean. Crop Sci, 1986,26:460-463.
[10] 崔章林,盖钧镒.世界大豆研究进展与动向.大豆科学,1995,14(2):167-173.
[11] Kitamura K. Breeding trials for improving the food-processing quality of soybeans. Trends in Food Sci and Techno, 1993,4:64-67.
[12] 丁安林,张艳,常汝镇,等.大豆脂肪氧化酶研究进展.大豆科学,1995,14(1):67-73.
[13] Theorell H, Holman R T, Akeson A. Crystalline lipoxidase. Acta Chem Scand, 1947,1:571.
[14] Christoper J P, Pistorius E K, Axelrod B. Isolation of an isozyme of soybean lipoxygenase. Biochem Biophy Acta, 1970,198:12.
[15] Axelrod B, Cheesborough T M, Laasko S. Lipoxygenase from soybeans. Meth Enzymology, 1981,71:441-451.
[16] 石胜尧,张延坤,郭大发,等.大豆脂肪氧化酶活性的测定.营养学报,1996,18(3):354-357.
[17] 傅翠真,徐文英.β胡萝卜素漂白实验测定大豆脂肪氧化酶活性的研究.中国粮油学报,1996,11(2):43-45.
[18] Kitamura.K.. Biochemical characterization of lipoxygenase lacking mutants, L-1-less, L-2-less and L-3-less soybeans. Agric Biol Chem, 1984,48(9):2339-2346.
[19] Seizo Yabuuchi, Richard M Lister, Bernard Axelrod, et al. Enzyme-Linked Immunosorbent Assay for the Determination of Lipoxygenase Isoenzymes in Soybean. Crop Science, 1982,22:333-337.
[20] Kato T, Ohta H, Tanaka K, et al. Appreciation of new lipoxygenases in soybean cotyledons after germ ination and evidence for expression of a major new lipoxygenase gene. Plant physiol, 1992,98:324-330.
[21] James M Narvel,Walter R Fehr, Linda C Weldon. Analysis of Soybean seed lipoxygenases. Crop Science Society of America, 2000,40:838-840.
[22] Kanofsky JR, Axelrod B.Singlet oxygen production by soybean lipoxygenase isozymes. J Biol Chem, 1986,261:1099-1104.
[23] 张利华,张志良,沈曾佑.不同物质对大豆幼苗化学发光的影响.华东师范大学学报(自然科学版),1997,4:80-86.
[24] Yasuo Kondo, Teruo Miyazawa, Junya Mizutani. Detection and time-course analysis of phospholipid hydroperoxide in soybean seedlings after treatment with fungal elicitor, by chemiluminescence-HPLC assay. Biochim Biophys Acta, 1992,1127:227-232.
[25] 苏震,徐文英,张仲纶,等.大豆种子脂肪氧化酶同工酶缺失体的超弱发光研究.大豆科学,1997,16(3):245-251.
[26] 徐文英,傅翠真,苏震.大豆脂肪氧化酶的研究动态.植物生理学通讯,1996,32(4):308-313.
[27] Shibata D, Steczko J, Dixon J E, et al. Primary structure of soybean lipoxygenase-1.Journal of Biology and Chemistry, 1987,262(21):10080-10085.
[28] Shibata D, Steczko J, Dixon J E, et al. Primary structure of soybean lipoxygenase-2.Journal of Biology and Chemistry, 1988,263:6816-6821.
[29] Richard L Y , Miriam F, Cecilia L. Isolation and characterization of a soybean lipoxygenase-3 gene. Molecular General Genetics, 1988,211:215-222.
[30] Wang C, Croft K P C, J?rlfors U, et al.Subcellular localization studies indicate that lipoxygenases 1 to 6 are not involved in lipid mobilization during soybean germination.Plant Physiology, 1999,120:227-235.
[31] Benjamin F Matthews, Thomas E, Devine, Jane M, et al. Incorporation of Sequenced cDNA and Genomic Markers into the Soybean Genetic Map. Crop Science, 2001,41:516-521.
[32] 孙君明,伍树明,陶文静,等.大豆脂肪氧化酶-1缺失基因（lx1）的RAPD标记.中国农业科学,2004,37(2):170-174.
[33] 段红梅,王文秀,常汝镇,等.大豆SSR标记辅助遗传背景选择的效果分析.植物遗传资源学报,2003,4(1):36-42.
[34] 邱丽娟,常汝镇,许占友,等.利用分子标记评价大豆种质的研究进展.大豆科学,1999,18(4):347-350.
[35] Hildebrand D F, Hymowitz T. Inheritance of lipoxygenase-1 activity in soybean seeds. Crop Science, 1982,22:851-853.
[36] Nielsen N C, Davies C S. Genetic Analysis of a Null-Allele for Lipoxygenase-2 in Soybean. Crop Science, 1986,26:460-462.
[37] 崔艳华,邱丽娟,常汝镇,等.黄淮夏大豆遗传多样性分析.中国农业科学,2004,37(1):15-22.
[38] 傅翠真.大豆脂肪氧化酶缺失体检测方法研究.大豆科学,2004,23(2):111-113.
[39] 张瑛,张磊,吴敬德,等.植物脂肪氧化酶同功酶快速检测技术在无豆腥味大豆育种上的应用研究.大豆科学,2003,22(1):50-53.
[40] 麻浩,官春云.大豆种子脂肪氧化酶缺失基因控制豆腥味效果的研究.中国农业科学,2001,34(4):367-372.