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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (9): 312-314.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

特种水生蔬菜刺苦草初代培养研究初探

陈 寒,徐文娟,李 勇,赵 迪   

  • 收稿日期:2008-04-18 修回日期:2008-05-20 出版日期:2008-09-05 发布日期:2008-09-05

Research on the Primary Culture of the Special Aquatic
Vegetable Vallisneria Spinulosa Yan

Chen Han, Xu Wenjuan, Li Yong, Zhao Di   

  • Received:2008-04-18 Revised:2008-05-20 Online:2008-09-05 Published:2008-09-05

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751

摘要： 选取优质的刺苦草的根状茎、芽、叶为外植体，以MS为基本培养基适时添加适量的生长素和细胞分裂素进行离体培养。结果表明：以芽为外植体，1%升汞消毒8min，以MS为培养基，添加0.2~2mg/LBA、0.2~2mg/LNAA、0.2~2mg/LIBA、蔗糖30g/L、琼脂10g/L，BA/NAA为3:1和1:1时和BA/IBA为1:1和1:10时，植株生长健壮，叶片颜色青翠，幼茎生长得又快又高。

关键词: 烟草, 烟草, 生防益菌, 促生

Abstract: The shoot tips of Vallisneria Spinulosa Yan were used as explants in the test of tissue culture and rapid propagation, the direct method of differentiation of young sprout by inducing embryoid was used with the basic MS culture medium with the different sorts and concentrations of phytohormone. The results showed that embryoid was induced and the young sprout formed from the plumules with the basic MS culture medium with 0.2-2mg/LBA, 0.2-2mg/LNAA, 0.2-2mg/LIBA, 30g/Lsugar, 10g/Lagar. When BA/NAA=3:1 and 1:1 or BA/IBA=1:1 and 1:10, the plants were getting the seeding that grew orderly and vigorously with fresh green leaves and higher leaf-like stems.

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