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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (3): 202-205.

• 植物保护 农药 • 上一篇    下一篇

合欢叶乙醇提取物对植物病原真菌的抑菌活性测定

金玉兰,曲田丽,肖琳   

  • 收稿日期:2008-11-14 修回日期:2008-12-02 出版日期:2009-02-05 发布日期:2009-02-05

Antifungal Activity of Ethanol Extract from Albizia julibrissin

  • Received:2008-11-14 Revised:2008-12-02 Online:2009-02-05 Published:2009-02-05

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摘要： 摘要: 本文通过测定合欢叶的乙醇提取物以及各萃取部分对植物病原真菌的抑菌活性，寻找合欢叶中的农用活性物质，为其综合开发利用提供科学依据。采用菌丝生长速率法测定了合欢叶（Albizia julibrissin）乙醇提取物对8种植物病原菌的抑制作用。结果表明，在供试浓度为5.0 mg•mL-1时，合欢叶乙醇提取物对苹果腐烂病菌（Valsa mali）、番茄灰霉病菌（Botrytis cinerea）、立枯丝核病菌（Rhizoctonia solani）和辣椒炭疽菌(Colletotrichum capsici)的抑菌活性较高，抑菌率分别为84.62、68.42、55.48和48.81%；EC50分别为0.516、1.110、6.630和9.770 mg•mL-1。合欢叶乙醇提取物的不同极性溶剂萃取物中正丁醇层与水层的抑菌活性较高。正丁醇层对苹果腐烂病菌的抑菌作用最好，EC50达0.012 mg•mL-1。活性物质主要集中于正丁醇萃取部分，是进一步研究的重点。

关键词: 棉花, 棉花, 抗旱品种, 节水技术, 关中东部

Abstract: Abstract: The inhibiting effects of ethanolic extract and fractions were determined from Albizia julibrissin against the plant pathogens. The aim was to develop and utilize comprehensively agricultural substances of A. julibrissin leaves . Using growth rate methods, antifungal activity of A. julibrissin leaves was researched in this experiment. The results showed that the ethanol extract from leaves of A. julibrissin was effective against Valsa mali，Botrytis cinerea，Rhizoctonia solani and Colletotrichum capsici. The inhibition rates were 84.62，68.42，55.4 and 48.81% at 5.00 mg•mL-1, respectively. The antifungal activities of n-hexane, ethyl acetate, n-butanol partition and water from ethanol extracts showed that the most activity components were distributed in n-butanol extracts. The EC50 of n-butanol partition were 0.012 mg•mL-1 against V. mali. The n-butanol was the most active partition and could be acted as the emphases for further study.