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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (10): 59-62.

所属专题： 小麦

• 生物技术科学 • 上一篇    下一篇

SSR标记分析小麦品种Flinor和铭贤169的遗传差异

左丽玲,冯晶,蔺瑞明,章振羽,曹远银,徐世昌   

  • 收稿日期:2008-12-09 修回日期:2009-01-10 出版日期:2009-05-20 发布日期:2009-05-20

The genetic difference of Flinor and Mingxian169 analysed by microsatell ite marker

  • Received:2008-12-09 Revised:2009-01-10 Online:2009-05-20 Published:2009-05-20

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摘要： 本研究采用微卫星（SSR）分子技术，对小麦品种Flinor与铭贤169染色体组进行SSR引物多态性频率分析。结果表明:在1243对SSR引物中共筛选到多态性引物383对，多态性频率为31.10℅；127对条带差异不清晰；无遗传多态性共有733对，占总筛选引物数的59.35℅。其中 BARC，GWM，CFA的多态性较高，多态性频率分别为34.98℅，34.72℅，36.49℅；CWEM多态性较差，多态性频率仅为8.89℅。多态性引物在不同染色体组间分布频率不同，A为41%,B为35%,D染色体组上多态性较少为24%。

关键词: 蟠桃, 蟠桃, 1-MCP, 营养成分, 生理效应, 酶活性

Abstract: Simple sequence repeat (SSR) molecular marker was used to analyze the frequency of polymorphism of wheat varieties – Flinor and Mingxian169. The result was followed. Of 1243 SSR markers used to investigate DNA polymorphism of the two cultivars, 383 markers could express the polymorphic and cover 31.10%, 127 markers had no clear band, and 733 markers had no polymorphic and covered 59.35%. The polymorphism of BARC, WMS and CFA was higher with 34.98%, 34.72 and 36.49, respectively, while the polymorphism of CWEM was lower with 8.89%. The distribution frequency of polymorphic markers was different among the chromosome in the A, B and D genome, the highest in A genome (41%), lower in B genome (35%) and the lowest in D genome (24%).

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