口蹄疫146S对乳鼠心肌膜微血管内皮细胞分泌IL-6的影响

中国农学通报 ›› 2008, Vol. 24 ›› Issue (4): 19-22.

所属专题：生物技术；畜牧兽医

口蹄疫146S对乳鼠心肌膜微血管内皮细胞分泌IL-6的影响

田丽芳,张涛,张爽,陈武,索占伟,胡格,段惠琴,穆祥

收稿日期:2008-02-26 修回日期:2008-03-04 出版日期:2008-04-05 发布日期:2008-04-05

Effects of FMDV 146S on IL-6 Release of Suckling Rat Myocardial Microvascular Endothelial Cells in Vitro

Tian Lifang, Zhang Tao, Zhang Shuang , Chen Wu, Suo Zhanwei, Hu Ge, Duan Huiqin, Mu Xiang

Received:2008-02-26 Revised:2008-03-04 Online:2008-04-05 Published:2008-04-05

摘要/Abstract

摘要： 研究口蹄疫146S病毒粒子抗原（FMDV 146S）对乳鼠心肌膜微血管内皮细胞（RMMVECs）分泌IL-6的影响，为解决现有口蹄疫疫苗的不足提供数据。体外培养乳鼠心肌膜微血管内皮细胞，用ELISA方法检测FMDV 146S处理后细胞上清液中IL-6浓度。乳鼠心肌膜微血管内皮细胞（RMMVECs）在正常状态下低水平分泌IL-6；FMDV 146S刺激后IL-6的分泌量显著增加，且呈剂量和时间依赖性，在刺激24 h后分泌量达到峰值。MVECs在FMDV 146S诱导的免疫与炎症反应中起着重要作用。

关键词: 奶牛, 奶牛, 温氏附红细胞体, 抗原, SDS-PAGE, 免疫印迹

Abstract: To study the effects foot and mouth diseases virus 146S particle antigen (FMDV 146S) on IL-6 release of sucking rat myocardial microvascular endothelial cells (RMMVECs) in vitro, and to provide data for solving the problem of side effects in using foot-and-mouth-disease vaccines. The cultured RMMVECs in vitro were identified as endothelial cells by morphology and by positive immunocytochemistry for factor Ⅷ-related antigen, a marker for endothelial cells. Using enzyme linked immunosorbent assay (ELISA) to detect the level of IL-6 produced by RMMVECs stimulated with FMDV 146S in cell supernatant liquid. On normal condition, RMMVECs can secrete IL-6 at low level. The level of IL-6 produced by RMMVECs stimulated with FMDV146S in cell supernatant liquid was increased dramatically in dose and time dependents, and got the maximum at 24 h. MVECs are very significant in immune and inflammatory reaction induced by FMDV 146S.

田丽芳,张涛,张爽,陈武,索占伟,胡格,段惠琴,穆祥. 口蹄疫146S对乳鼠心肌膜微血管内皮细胞分泌IL-6的影响[J]. 中国农学通报, 2008, 24(4): 19-22.

Tian Lifang, Zhang Tao, Zhang Shuang , Chen Wu, Suo Zhanwei, Hu Ge, Duan Huiqin, Mu Xiang. Effects of FMDV 146S on IL-6 Release of Suckling Rat Myocardial Microvascular Endothelial Cells in Vitro[J]. Chinese Agricultural Science Bulletin, 2008, 24(4): 19-22.

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口蹄疫146S对乳鼠心肌膜微血管内皮细胞分泌IL-6的影响

Effects of FMDV 146S on IL-6 Release of Suckling Rat Myocardial Microvascular Endothelial Cells in Vitro

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