关键词: 园艺植物, 园艺植物, 原生质体技术, 原生质体融合, 育种

Abstract: Abstract：Distant hybridization based on the use of wheat (Triticum aestivum L.) and maize (Zea mays L.) is a important way of obtaining the haploid wheat. It is the double haploid plants are received and a high efficiency way to heredity population of wheat. which is used to construct the resistant midge DH population of wheat, in the greenhouse in the experiment of 5 groups of Resistance, susceptible wheat midge hybrid in order to induce the formation of the wheat haploid. Turns out: different types of genes have much influence on inducing the formation of wheat haploid; the suitable time to the pollinated wheat is 6-36 hours; the suitable amount of 2.4-D drug is 10-30mg/L; The appropriate time for stripping embryo culture is 14-16 days. The stem has the good inducement effect under the isolated culture condition after the pollination. The appropriate temperature is 20-250C. Methods and conditions for inducing the wheat haploid plants in a greenhouse are given in this study.