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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (15): 0-1.

所属专题： 生物技术

• 生物技术科学 •    下一篇

拟南芥基因组 NBS-LRR类基因家族的生物信息学分析

王岩   

  • 收稿日期:2009-03-20 修回日期:2009-04-18 出版日期:2009-08-05 发布日期:2009-08-05

Bioinformatic Analysis of the NBS-LRR Gene Family in Arabidopsis thaliana

wang yan   

  • Received:2009-03-20 Revised:2009-04-18 Online:2009-08-05 Published:2009-08-05

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摘要： 【研究目的】本研究对拟南芥（Arabidopsis thaliana）中含有核苷酸结合位点（Nucleotide binding site，NBS）和富含亮氨酸的重复序列（Leucine-rich repeat，LRR）类基因进行了分析。【方法】利用TAIR、Pfam网站,数据库和Chromosome Map Tool、MEGA 3.0、ClustalX软件，分析确定NBS-LRR基因及其类型、染色体物理分布、系统发育学关系。【结果】在拟南芥全基因组中共有204 个NBS-LRR 类基因，大多位于1号和5号染色体上，其中71.6%的NBS-LRR 类基因分布在基因簇内。对NBS-LRR 类基因家族进行了氨基酸多序列比对和系统进化树分析，NBS-LRR 类基因家族可分为四个亚家族。另外，NBS-LRR基因在进化过程中存在较多的复制现象。

关键词: 烤烟, 烤烟, 基因型, 生物量, 主侧根发育, 根系活力, 叶绿素

Abstract: Abstract: 【OBJECTIVE】A full set of disease resistance candidate genes encoding NBS-LRR in a complete genome of Arabidopsis thaliana were analyzed. 【METHOD】 Structural diversities, physical positions, and phylogenetic relationship were studied by TAIR and Pfam web, database, Chromosome Map Tool, MEGA 3.0 and ClustalX software. 【RESULTS】 204 genes containing NBS domain were found in genome and most of those were located in 1 and 5 chromosome. The NBS-LRR family genes on different chromosomes exist in the form of monogenic gene or gene clusters, and 71.6% of the NBS-LRR genes were located in gene clusters. That showed there are much copy phenomenons in the evolution of disease resistance genes of Arabidopsis thaliana.