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中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 155-160.

所属专题: 玉米

• 农学 农业基础科学 • 上一篇    下一篇

固定根区水分胁迫下氮形态对玉米幼苗水分利用的调节与作用机制

王海红 束良佐 周秀杰 祝鹏飞 刘方东   

  • 收稿日期:2009-06-19 修回日期:2009-07-07 出版日期:2009-09-20 发布日期:2009-09-20
  • 基金资助:

    局部根区水分胁迫下氮形态与供应部位对植物干旱胁迫反应的调节

Regulation Mechanisms of Nitrogen Form on Water Utilization of Maize Seedlings under Fixed Partial Root-zone Water Stress

  • Received:2009-06-19 Revised:2009-07-07 Online:2009-09-20 Published:2009-09-20

摘要:

摘 要:为研究固定根区水分胁迫下氮形态对玉米幼苗水分利用的调节与作用机制,本文采用分根装置,向一侧加入聚乙二醇(PEG6000)模拟水分胁迫,分两次收获植株测定木质部水分运输速率、生理水分利用效率以及不同部位脱落酸含量等指标,并在第7天测定叶片及根系解剖结构。氮设三种形态(NH4+-N;NO3--N;50% NO3--N+50% NH4+-N),两侧根室均匀供应。结果发现固定根区水分胁迫下,随着胁迫时间延长,混合氮处理能相对维持植株较高的水分运输速率;叶肉和叶片厚度大,导管数目较多,直径较大,有利于促进水分吸收与运输,适应水分胁迫。而NH4+-N对木质部汁液pH及ABA的调节作用较强,各部位ABA浓度高于其他两个氮形态;在胁迫后期NH4+-N供应的植株木质部汁液pH值增加,从而更有利于调节蒸腾作用,提高水分利用效率。

关键词: 烤烟, 烤烟, 自动控温控湿烤房, 烘烤工艺

Abstract:

Abstract: In order to investigate the regulation mechanisms of nitrogen form on water utilization of maize seedlings under fixed partial root-zone water stress conditions, a split-root system composed of two root compartments was used. Polyethylene glycol (PEG 6000) was added to one root compartment simulating fixed partial root-zone water stress. Physiological indices, such as xylem water transportation rate, physiological water use efficiency and abscisic acid concentration in different parts of plant were examined at different time after water stress treatments. Nitrogen was set as three forms (50%NO3--N+50%NH4+-N;NO3--N;NH4+-N), and added to two root compartments homogenously. It showed that, under fixed partial root-zone water stress conditions, plant supplied with mixed nitrogen could maintain relative higher water transportation rate as time of water stress prolonged comparing with NH4+-N and NO3--N supplement. Thickness of mesophyll and leaf, vessel number and diameter in plant supplied with mixed nitrogen was also high. These traits could adapt plants to water stress by improving water absorption and translocation. However, ammonium nutrition had a greater effect on the increment in xylem sap pH and ABA concentration in different parts of plant compared to other nitrogen sources. Xylem sap pH in plant supplied with ammonium increased evidently at late stage of the water stress treatment, which could enhance the regulation of plant transpiration. Ammonium nutrition improved water use efficiency of maize seedlings under fixed partial root-zone water stress.