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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (2): 234-237.

• 植物保护 农药 • 上一篇    下一篇

应用60Coγ射线诱变选育武夷菌素高产菌的研究

程艳1,2,张克诚2,赵明富1,石义萍2,孙蕾2   

  • 收稿日期:2009-08-31 修回日期:2009-10-13 出版日期:2010-01-20 发布日期:2010-01-20
  • 基金资助:

    公益性行业（农业）科研专项资金项目“生态康复型农田绿色控害技术研究”;国家科技支撑计划课题“农林重大生物灾害防控技术研究”

Research On High-Yield Strain of Wuyiencin By 60Co γ Ray Mutagenesis

  • Received:2009-08-31 Revised:2009-10-13 Online:2010-01-20 Published:2010-01-20

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摘要：

摘要：为进一步提高农用抗生素武夷菌素产生菌药效，降低生产成本，本实验以现发酵生产上使用的武夷菌素产生菌L7为出发菌株，采用三种不同剂量（500Gy,1000Gy,1500Gy）的60Coγ射线对L7菌进行诱变处理。结果表明，辐射剂量为1000戈瑞时L7菌株的孢子死亡率为98.7%，正变率为18.9%，是武夷菌素产生菌诱变选育的最佳剂量。从3582株突变株中经琼脂块初筛选育出78株相对高产菌株进行复筛，通过对发酵滤液效价的测定，有20株效价超过5000μg/ml，从中选出5株效价最高的菌株（C-28，C-42，C-64，C-75，C-76）进行遗传稳定性测定。五次传代后，结果显示，菌株C-75遗传稳定性较好，能够稳定保持高效价不变（达到5528μg/ml），较出发菌株L7（效价4112μg/ml）提高了34.4%。其余四株均有不同程度的菌株退化现象，最终选定C-75菌株为本次60Coγ射线诱变选育的武夷菌素高产菌株。

关键词: 棉花, 棉花, 叶龄, 相对株高, 模拟模型, 诊断指标, 预测方法

Abstract:

Abstract: Three types 60Co γ Rays are applied on the spores based on the experiments on present high-yield L7 strains to lower the cost and make the Wuyiencin more effective. In the event that 1000 grays are adopted, 98.7% of the spores die and 18.9% mutate. It shows 1000 grays of Wuyiencin are the most appropriate for testing. 78 strains out of 3,582 are selected by screening of agar blocks, and then fermented and screened once again, among which 20 strains are tested above 5000μg/ml in tilter, in which 5 strains ( i.e. C-28，C-42，C-64，C-75，C-76) are tested as to genetic stability.As a result, C-75 strain is the most stable genetically with 5528μg/ml of titer, 34.4% higher than L7 strains (4112μg/ml of liter) and therefore is determined as high-yield producing strain of Wuyiencin by 60Co γ ray mutagenesis. By contrast, the other four strains degenerate to different degrees.