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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (24): 382-385.

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

除虫菊内生拟盘多毛孢Y1菌株发酵产物抑菌活性初步研究

易晓华   

  • 收稿日期:2009-09-10 修回日期:2009-09-30 出版日期:2009-12-20 发布日期:2009-12-20
  • 基金资助:

    国家“十五”科技攻关重大专项资助项目“无公害农药关键技术与产品开发”

The Preliminary Study on Antifungal Activity of Fermentation Products of Endophytic Fungi Y1(Pestalotiopsis sp.) from Pyrethrum cinerariifolium

  • Received:2009-09-10 Revised:2009-09-30 Online:2009-12-20 Published:2009-12-20

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摘要：

摘　要:在对从除虫菊中分离到的内生真菌进行抑菌活性系统筛选的基础上,对其中活性较高的Y1 （Pestalotiopsis sp.）菌株进行了抑菌活性测定。其发酵液10倍稀释液和菌丝丙酮提取物对玉米大斑病菌 Exserohilum turcicum等 6种供试植物病原真菌菌丝生长的抑制率分别在85. 2%～95.1%之间和40%～80%之间,其 5倍稀释液对番茄灰霉病菌 Botrytis cinerea和苹果炭疽病菌 Glomerella cingulata孢子萌发的抑制率均大于 82%。活体生测结果表明,该发酵液对番茄灰霉病，辣椒疫霉病和黄瓜霜霉病的保护效果大于 55%,治疗效果不明显。

关键词: 叶面营养, 叶面营养, 磷酸二氢钾, 锌, 锰, 冬小麦

Abstract:

Abstract: Based on the antifungal activity screening of endophytic fungi obtained from Pyrethrum cinerariifolium, the strain of Y1 with higher activity its active substances were separated. The bioassay results showed that the inhibition rates of 10 time diluted fermentation liquid of Y1 and the endophytic fungi mycelium extract by ethyl acetate against 6 pathogenic fungi including Exserohilum turcicum et al. were ranged from 85. 2% to 95.1% and from 40% to 80%, and that of the 5 time diluted fermentation liquid of Y1 against Botrytis cirerea and Glomerella cingulata were more than 82%. The antifungal activity of fermentation product of Y1 were analyzed by in vivo assay, the protective effect against B. cinerea ，Phytophthora capsici and Lycopersicon esculentum were more than 55%, the therapeutic effectwere not prom inent.