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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (24): 50-55.

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

稻瘟病菌Subtilases家族生物信息学分析及其中一蛋白的分泌性检验

魏艺聪1,王宗华2,3,鲁国东2   

  • 收稿日期:2009-11-04 修回日期:2009-09-24 出版日期:2009-12-20 发布日期:2009-12-20

Bioinformatics Analysis of Sublitases in M. grisea and Secretion detection of MGG_07965.6

  • Received:2009-11-04 Revised:2009-09-24 Online:2009-12-20 Published:2009-12-20

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摘要：

根据已有的研究结果推测稻瘟病菌Subtilases家族基因可能在其致病过程中具有重要的功能，本研究以进化理论为基础，结合一些生物信息学的软件对稻瘟病菌基因组中Subtilases家族基因进行了比较基因组、进化分析及功能域分析，分析了Subtilases家族对稻瘟病菌致病性的重要性，并推测家族中不同基因的结构、功能及参与的生理过程可能的异同之处，以基因MGG_07965.6为例，分析其可能具有的生物学功能，并通过实验的方法证实了其基因表达产物确是分泌蛋白。

关键词: 红厚壳, 红厚壳, 生长量, 气象条件, 指标

Abstract:

Previous researches have suggested that the subtilases gene of Magnaporthe grisea may play important roles in pathogenesis, In this paper, bioinformatic tools and genome database were used to investigate the function of sublitases in Magnaporthe grisea. By oncogenomic and phylogenetic analyses of sublitases, we hypothesized that some of the sublitases may control M. grisea pathogenecity . The genes function and physiological roles were also speculated according to the genes structure. The MGG_07965.6 gene was as an example for further study. The protein product of MGG_07965.6 gene was confirmed to secrete outside of the fungal cells.The functional genomic analysis methods were tested in this study for M. grisea based on the evolutionary theory and bioinformatics softwares.