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中国农学通报 ›› 2010, Vol. 26 ›› Issue (8): 84-88.

所属专题: 生物技术 园艺

• 生物技术科学 • 上一篇    下一篇

番茄八氢番茄红素脱饱和酶基因(Pds)启动子的克隆

万群   

  • 收稿日期:2009-11-09 修回日期:2009-12-13 出版日期:2010-04-20 发布日期:2010-04-20

Cloning of Phytoene desaturase(Pds) promoter

Wan Qun   

  • Received:2009-11-09 Revised:2009-12-13 Online:2010-04-20 Published:2010-04-20

摘要:

根据GenBank中番茄八氢番茄红素脱饱和酶基因(Phytoene desaturase.Pds)的启动子序列(U46919),设计特异引物,通过巢式PCR从番茄基因组中分离了一段长1790bp的Pds启动子序列。将该片段插入到克隆载体pMD18-T中,转化大肠杆菌后,获得的阳性克隆经测序,结果表明所获得的序列和GenBank(U46919)中登录的序列完全一致

关键词: 白灵菇, 白灵菇, 生物量, 营养物质, 培养条件, 优化

Abstract:

the DNA fragment of tomato phytoene desaturase gene promoter (Pds) was isolated from the tomato genome DNA through the nested PCR. Sequence analysis showed that the sequence of the promoter is the same with the sequence published on the GenBank(U46919).