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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (19): 31-33.

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

广东湛江杂草稻qSH1基因片段序列分析

王黎明 陈勇 王林   

  • 收稿日期:2010-04-19 修回日期:2010-05-26 出版日期:2010-10-05 发布日期:2010-10-05

Sequence Analysis of qSH1 Gene Fragments of Weedy Rice from Zhanjiang of Guangdong Province

  • Received:2010-04-19 Revised:2010-05-26 Online:2010-10-05 Published:2010-10-05

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摘要：

摘要：为了探讨杂草稻与栽培稻在qSH1基因片段是否存在差异。利用已经报道的扩增水稻qSH1基因的引物，对来源于广东湛江雷州地区的6份落粒性非常强的杂草稻进行PCR检测，通过测序得到约460bp的核苷酸碱基序列，序列分析结果表明：该核苷酸碱基序列大致位于qSH1基因下游100kb处，与NCBI网站公布的核苷酸序列为同源序列，同源性达100 %。这6份杂草稻与水稻之间共同存在一个碱基位点差异，经证实正是前人报道的SNP位点。另外6份杂草稻所获片段序列不完全一致,存在一个位点差异，但不能确定是否为一个作用位点。

关键词: 土壤, 土壤, 肥力, 变化, 分析

Abstract:

Abstract: For the purpose to explore whether differences exist in qSH1 gene between weedy rice and Oryza sativa. Using the primers that had been used to amplify the qSH1 gene of Oryza sativa, PCR was done with 6 samples of weedy rices origin from Zhanjiang of Guangdong province, the qSH1 gene fragments were sequenced, the result of sequences alignment showed that the nucleotide sequence locates at region approximately 100kb downstream of qSH1, and was homologous with what has collected on the NCBI website, the homology value was up to 100%. There was a nucleotide site difference between the 6 weedy rices and Oryza sativa, which was proved to be the SNP site that reported before. At the same time, not all the nucleotide sequences that derived from the 6 weedy rices are the same, there is a different nucleotide site, but can’t determine whether it is an action site.