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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (19): 423-425.

所属专题： 生物技术

• 水产 渔业 • 上一篇    下一篇

Cu2+离子对水丝蚓的急性毒性及超氧化物歧化酶活性的影响

柳郁滨 范学铭 王哲娟   

  • 收稿日期:2010-04-21 修回日期:2010-06-05 出版日期:2010-10-05 发布日期:2010-10-05

Effect of Cu2+ on Limnodilus claparedianu Acute Toxicity and Superoxide Dismutase Activity

  • Received:2010-04-21 Revised:2010-06-05 Online:2010-10-05 Published:2010-10-05

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摘要：

采用急性毒性方法，测定了不同浓度的Cu2+离子溶液处理后的水丝蚓48h半致死浓度(LC50) 为8.640ul/L；用测定酶活性方法检测了中毒后的水丝蚓的超氧化物歧化酶(SOD)活性的变化：与对照组比较，Cu2+离子对动物SOD活性影响显著(p＜0.05).低浓度药物0.500ul/L～5.340 ul/L处理动物，SOD活性呈上升趋势；当浓度增加至11.740ul/L时，SOD活性开始表现为不同程度的降低. 说明Cu2+离子对水丝蚓造成胁迫，直至动物个体死亡，间接或直接地影响水生生态系统及以水丝蚓为食物的下一级消费者。

关键词: 农药残留, 农药残留, 现状, 对策

Abstract:

The author of this article adopted an acute toxicity method to mensurate the semi-lethal concentration (LC50) of Limnodilus claparedianus that were disposed in Cu2+ with different concentrations. The variation of L. claparedianus SOD activity toxic was tested by means of enzyme activity in this paper. The results showed that the semi-lethal concentration (LC50) of 48 hours was 8.640 ul/L. Cu2+ had a significant effect on L. hoffmeisteri SOD activity (P＜0.05). The SOD activity in the animal specimen increased when they were disposed in low concentration Cu2+ (0.500ul/L～5.340 ul/L). But SOD activity in the animal specimen decreased at different levels when concentrations of Cu2+ increased to 11.740ul/L, a conclusion was achieved that Cu2+ could cause coercion to L. claparedianus and even cause death of animal individuals, and they could also lead to direct and indirect influence on aquatic ecosystems and the subspecies which were fed by L. claparedianus.