关键词: 葡萄, 葡萄, 稻草, 玉米秸秆, 养分, 微生物

Abstract:

Abstract：According to the published gene sequence of Transmissible gastroenteritis Virus of pigs(TGEV) and Porcine epidemic diarrhea Virus(PEDV) and the detecting methods of RT-PCR reported,matrix gene of TGEV and nucleoprotein(NP) gene of PEDV were considered as the target gene sequences of multiple RT-PCR.A set of universe primers were designed for detecting TGEV and PEDV,respectively in the conseved S gene of TGEV and S gene of PEDV.The products of RT-PCR is 426 bp and 584 bp respectively.The optimal condition of multiple RT-PCR for detection of TGEV and PEDV by RT-PCR was determined through orthogonal assay.The specificity of the multipie primers were examined by RT-PCR using template extracted from other avian virus.The sensitivity of RT-PCR were also determined by a seral dilution of RNA from TGEV and PEDV.This study have paved the way for testing TGEV and PEDV,defining pathogenic potential,using vaccines against TGEV and PEDV and so on. Key words：Transmissible gastroenteritis Virus；Porcine epidemic diarrhea Virus；RT-PCR