关键词: 多模型, 多模型, 农产品, 供求信息, 预测

Abstract:

Based on the conserved regions of known α-gliadin genes, gene-specific primers for α-gliadin were designed to amplify the full gene coding regions from the DNA of wheat cultivar‘Chengdianmai 1’, which was developed by wheat-Secale wide cross and chromosome manipulation. The PCR products were cloned and sequenced. Total 6 sequences were obtained with length of 978 to 1310 bp, in which CD-1, CD-9, CD-11, CD-12, CD-17 and CD-12 were 1207 bp, 978 bp, 1310 bp, 1258 bp, 1210 bp, 1218 bp, respectively. Three of them were full ORF sequences (CD-1, CD-12 and CD-17), containing the coding regions of 284~301 amino acids, and the other three were pseudo genes. Multi-alignment analysis indicated that three full ORF sequences had higher similarity with other α-gliadin genes, and the differences mainly existed in the two polyglutamine regions and the repetitive domains. CD-12 is lack of the Glia-α20 epitope, whereas CD-1 and CD-17 only contained the Glia-α9 and Glia-α20 epitopes. For the two polyglutamine regions, length of sequences was strongly different and some glutamines were replaced by other residues. The coposition and number of the cystenin residue of the sequences indicated that the CD-1 and CD-12 exhibited unique characters comparing with the other reported α-gliadin genes. The phylogenetic tree indicated that all the full α-gliadin gene ORF sequences from‘Chengdianmai’1 could not be clustered into the group of α-gliadin genes from the A, B and D genomes of wheat (Triticum aestivum ), but presented in a new group, indicating the great gliadin genes divergence during the breeding program of chromosome engineering. The role of these genes in wheat quality and breeding needed to be further studied.