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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (9): 395-399.

• 植物保护 农药 • 上一篇    下一篇

福建省致病疫霉菌SRAP遗传多样性分析

兰成忠 李本金 陈庆河 翁启勇   

  • 收稿日期:2010-09-09 修回日期:2010-10-13 出版日期:2011-04-25 发布日期:2011-04-25
  • 基金资助:

    国家自然科学基金“致病疫霉菌的群体遗传结构及其变异机制”;马铃薯晚疫病菌无毒基因的变异及分子进化机制;马铃薯晚疫病菌无毒基因的变异及分子进化机制;重要作物疫病菌毒性组成结构及其抗药性监测研究

Analysis on Genetic Diversity of Phytophthora infestans Based on SRAP in Fujian Province

  • Received:2010-09-09 Revised:2010-10-13 Online:2011-04-25 Published:2011-04-25

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摘要：

为了解福建省致病疫霉菌的群体遗传结构，为该病原菌的遗传进化提供理论依据，笔者应用SRAP分子标记技术对福建省致病疫霉菌的群体遗传多样性，及不同地区菌株间的关系进行比较分析。利用10个菌株从110对引物组合中筛选出多态性引物10对，对分离自福建省10个不同市（县）的62个致病疫霉菌菌株DNA进行PCR扩增，共产生92条谱带，其中多态性标记90条，多态检测率为97.8%。利用NTSYpc Version2.1软件对供试菌株间的遗传距离进行聚类分析并构建系统树状图。以遗传距离0.57为阈值，可将供试62个菌株划分为4个遗传聚类组，SRAP分组与菌株的地理来源、寄主均无明显相关性。聚类分析结果表明，福建省不同地区的致病疫霉菌整体亲缘关系相近，但各菌株间存在遗传差异。

关键词: 广玉兰, 广玉兰, 植物病原真菌, 抑菌活性, 苹果腐烂病菌, 白菜黑斑病菌

Abstract:

In order to learn the genetic structure of Phytophthora infestans in Fujian province and provide a theoretical basis for genetic evolution of this pathogen. Sequence related amplified polymorphism (SRAP) analysis was used to detect the genetic variation in populations of Phytophthora infestans in Fujian province. Among 110 combinations of primer pairs screened, 10 primer pairs that amplified consistent polymorphic bands with DNA of 10 isolates were generated. They were further applied to amplify the genomic DNA of 62 isolates collected from different areas and hosts in Fujian province. In total, 92 SRAP markers were obtained. Among the bands, 90 were polymorphic, which accounted for 97.8%. Based on the genetic distance, a dendrogram was constructed with the software NTSYSpc Version2.1. At genetic distance of 0.57, 62 isolates were divided into 4 SRAP groups. There was no correlation between SRAP group and the geographic origin or their hosts. The analysis showed that some strains from one area closely related to that from another area. Nonetheless, rich genetic variation existed among the tested strains in Fujian province.